Serum exosomal microRNA transcriptome profiling in subacute spinal cord injured rats

Ding, Songtao; Chen, Yuqing; Chen, Jing; Wang, Sainan; Duan, Fei‐Xiang; Shi, Yujiao; Hu, Jianguo; Lü, He‐Zuo

doi:10.1016/j.ygeno.2019.12.003

Cited by 17 publications

(12 citation statements)

References 136 publications

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“…Predictive of AIS conversion in patients Dalkilic et al, 2018 [ 10 •], Kwon et al, 2017 [ 67 ], Yang et al, 2018 [ 69 ], de Mello Rieder et al, 2019 [ 76 ] MCP-1 Inflammation Elevated early after SCI. Results about prognostic utility are inconsistent Dalkilic et al, 2018 [ 10 •], Kwon et al, 2017 [ 67 ], Casha et al, 2018 [ 72 ], Heller et al, 2017 [ 77 ] Albumin Hepatic synthesis Hypoalbuminemia after SCI was associated with poor long-term neurological outcome Tong et al, 2018 [ 78 ], Vo et al, 2020 [ 79 ] microRNAs Various Severity-dependent distinct changes in the expression profile after SCI with up- and downregulation of certain microRNAs Sun et al, 2018 [ 80 ], Ding et al, 2020 [ 81 ], Park et al, 2019 [ 82 ], Li et al, 2019 [ 83 ], Tigchelaar et al, 2019 [ 84 ] Overview of serum/CSF biomarkers with their pathophysiological origin and a short summary of their diagnostic/prognostic utility after SCI …”

Section: Markers In Cerebrospinal Fluid and Blood Serummentioning

confidence: 99%

“…Following SCI, some of these microRNAs are upregulated leading to a decrease in expression of their target genes while others are downregulated. This in turn leads to a change in the microRNA expression profile and thereby to a different measurable spectrum of microRNAs in blood and CSF [ 80 , 81 ]. This expression profile varies over time after SCI providing the possibility of characterizing each post-injury stage [ 82 , 83 ].…”

Section: Micrornasmentioning

confidence: 99%

See 1 more Smart Citation

Improving Diagnostic Workup Following Traumatic Spinal Cord Injury: Advances in Biomarkers

Schading

Emmenegger

Freund

2021

Curr Neurol Neurosci Rep

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Purpose of Review Traumatic spinal cord injury (SCI) is a life-changing event with drastic implications for patients due to sensorimotor impairment and autonomous dysfunction. Current clinical evaluations focus on the assessment of injury level and severity using standardized neurological examinations. However, they fail to predict individual trajectories of recovery, which highlights the need for the development of advanced diagnostics. This narrative review identifies recent advances in the search of clinically relevant biomarkers in the field of SCI. Recent Findings Advanced neuroimaging and molecular biomarkers sensitive to the disease processes initiated by the SCI have been identified. These biomarkers range from advanced neuroimaging techniques, neurophysiological readouts, and molecular biomarkers identifying the concentrations of several proteins in blood and CSF samples. Some of these biomarkers improve current prediction models based on clinical readouts. Validation with larger patient cohorts is warranted. Summary Several biomarkers have been identified—ranging from imaging to molecular markers—that could serve as advanced diagnostic and hence supplement current clinical assessments.

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Section: Markers In Cerebrospinal Fluid and Blood Serummentioning

confidence: 99%

Section: Micrornasmentioning

confidence: 99%

Improving Diagnostic Workup Following Traumatic Spinal Cord Injury: Advances in Biomarkers

Schading

Emmenegger

Freund

2021

Curr Neurol Neurosci Rep

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“…Lu et al observed that the serum miR-10b-3p aberrant expression in ESCC is correlated with lymph node metastasis and poor prognosis. Mechanistically, this study demonstrated that miR-10b-3p increased FOXO3 expression via targeting the 3'-untranslated region [13]. Those studies demonstrated that miRNAs could be used as markers for ESCC lymph node metastasis and prognosis.…”

Section: Introductionmentioning

confidence: 55%

Serum exosomal miR-4787-3p as potential lymph node metastasis and prognostic marker in esophageal squamous cell carcinoma.

Liu

Lin

Wang³

et al. 2021

Preprint

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Background: To explore the miR-4787-3p expression levels in the serum exosome and tissue and its role in lymph node metastasis and prognosis in ESCC. Methods: The miRNA array was conducted to detect the ESCC serum exosomal miRNAs expression. A receiver operating characteristic (ROC) curve was constructed to determine the predictive ESCC with lymph node metastasis efficacy of serum exosomal miR-4784-3p. The Cox regression analysis was preformed to explore prognostic factors for ESCC. Transwell assay and CCK-8 assays were utilized to evaluate cell migration, invasion, and proliferation, respectively. Results: High serum exosomal miR-4787-3p expression was demonstrated in lymph node metastasis group (P =0.011). The serum exosomal miR-4787-3p expression was significantly associated with histologic grade (P = 0.010), and TNM stage (P = 0.033). However, there was no significant relationship between tissue miR-4787-3p expression and clinical characteristics (P >0.05). ROC analyses revealed that the AUCs of serum exosomal miR-4787-3p for lymph node metastasis prediction was 0.787. The Cox regression analysis found that high expression serum exosomal miR-4787-3p were correlated with poor prognoses (for OS, HR=2.68, 95% CI: 1.02~7.04; for DFS, HR = 2.65, 95% CI: 1.05~6.68). Nevertheless, no association between tissue miR-4787-3p expression and ESCC prognosis. In addition, upregulated expression of miR-4787-3p could promote migration and invasion in vitro. Conclusions: Serum exosomal miR-4787-3p can be promising biomarkers for ESCC metastasis and prognosis

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“…Especially 16 miRNAs, such as miR-485, miR-30b, and miR-26b were consistent with reported miRNAs in the circulation, which means these molecules are related to the pathological processes. 136 In a SCI rat model, intravenous injection of MSC-EVs (instantly after attack and 24h later) showed an anti-inflammatory effect, yet the possible mechanism is interaction of EVs with activated primary microglia, thus inhibiting proinflammatory cytokine (IL-1β, IL-6) expression. 137 Research has shown that phosphatase and tensin homolog (PTEN) can negatively regulate neuronal regeneration, which can be suppressed by retinoic acid receptor β.…”

Section: Spinal Cord Injury (Sci)mentioning

confidence: 99%

Recent Advances on Extracellular Vesicles in Central Nervous System Diseases

Jin¹,

Gu²,

Li³

et al. 2021

CIA

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Extracellular vesicles (EVs) are particles released by multiple cells, encapsulated by lipid bilayers and containing a variety of biological materials, including proteins, nucleic acids, lipids and metabolites. With the advancement of separation and characterization methods, EV subtypes and their complex and diverse functions have been recognized. In the central nervous system (CNS), EVs are involved in various physiological and pathological processes, such as regulation of neuronal firing, synaptic plasticity, formation and maintenance of myelin sheath, propagation of neuroinflammation, neuroprotection, and spread and removal of toxic protein aggregates. Activity-dependent alteration of constituents enables EVs to reflect the change of cell and tissue states, and the wide distribution of EVs in biological fluids endows them with potential as diagnostic and prognostic biomarkers for CNS diseases, including neurodegenerative disease, cerebrovascular disease, traumatic brain disease, and brain tumor. Favorable biocompatibility, ability of crossing the blood-brain barrier and protecting contents from degradation, give promising therapeutic effects of EVs, either collected from mesenchymal stem cells culture conditioned media, or designed as drug delivery vehicles loaded with specific agents. In this review, we summarized EVs' basic biological properties, and mainly focused on their applications in CNS diseases.

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Serum exosomal microRNA transcriptome profiling in subacute spinal cord injured rats

Cited by 17 publications

References 136 publications

Improving Diagnostic Workup Following Traumatic Spinal Cord Injury: Advances in Biomarkers

Improving Diagnostic Workup Following Traumatic Spinal Cord Injury: Advances in Biomarkers

Serum exosomal miR-4787-3p as potential lymph node metastasis and prognostic marker in esophageal squamous cell carcinoma.

Recent Advances on Extracellular Vesicles in Central Nervous System Diseases

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