Serum-free cell culture

Bjare, Ulf

doi:10.1016/0163-7258(92)90056-6

Cited by 72 publications

(50 citation statements)

References 93 publications

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“…Insulin, the first of the components of the ITS supplement, has been known to be essential in cell culture from 1924 and is now the most commonly used hormone in culture media (Gey and Thalhimer, 1924). Transferrin is also an essential protein in culture medium where the main action is to transfer iron into the cells (Bjare, 1992). Selenium is an essential trace element and acts in particular in selenoproteins which protect cells against oxidative stress (Helmy et al, 2000).…”

Section: Basal Mediummentioning

confidence: 99%

“…The pioneering work by Hayashi and Sato (1976) replacing serum by the addition of selected hormones, promoting growth and stimulating differentiation of specific cells, led to the development of a good chemically defined, serum-free media (see Box 1) (Barnes and Sato, 1980a,b;Bjare, 1992;Grillberger et al, 2009;Gstraunthaler, 2003;Taub, 1990). In the last 10 years, investigations into cell function have led to the identification of a growing number of components which have been useful in the development of modern serum-free cell culture media.…”

Section: Development Of a Serum-free Mediummentioning

confidence: 99%

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Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Valk

Brunner

Smet³

et al. 2010

Toxicology in Vitro

499

379

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Section: Basal Mediummentioning

confidence: 99%

Section: Development Of a Serum-free Mediummentioning

confidence: 99%

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Valk

Brunner

Smet³

et al. 2010

Toxicology in Vitro

499

379

View full text Add to dashboard Cite

“…From a cell biological perspective, the disadvantages of supplementation of culture media with FBS are manifold: (i) Serum is an ill-defined medium supplement, and thus an ambiguous factor in cell culture (Bjare, 1992;Gstraunthaler, 2003), (ii) serum batches display quantitative and qualitative variations in their composition and thus introduce a serious lot-to-lot variability (Price and Gregory, 1982), (iii) serum may contain different amounts of endotoxins, haemoglobin, and other adverse factors, and (iv) serum can be a potential source of microbial contaminants, such as fungi, bacteria, mycoplasma, viruses or prions (Dormont, 1999;Eliot, 1999;Wessman and Levings, 1999). thus, serum introduces several unknown variables into the tissue culture system (even et al, 2006;lindl und Gstraunthaler, 2008;Stoll and Spector, 1984).…”

Section: A) Cell Biological Perspectivementioning

confidence: 99%

“…Additional methods of choice for reducing the requirement of FBS include (i) the optimisation of existing culture media, (ii) the use of reduced serum media, (iii) the design of chemically defined, serum-free media Sato, 1980a, 1980b;Barnes et al, 1987;Bjare, 1992;Defrancesco, 1998;Froud, 1999;Gstraunthaler, 2003;Jayme et al, 1988;Taub, 1990;Zimmermann et al, 2000) or (iv) the establishment of databases for serum-free culture media applications (Falkner et al, 2006) in order to provide well focused information to ease the use of and the switch to serum-free cell and tissue culture, respectively.…”

Section: Structure Of the Databasementioning

confidence: 99%

Serum-free cell culture: the serum-free media interactive online database

Brunner

Frank²,

Appl³

et al. 2010

ALTEX

324

232

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Prefacethe supplementation of basal culture media with animal serum of different origins is essential for cell growth, metabolism, and to stimulate proliferation ("mitogenic effect"). the sera used most widely are bovine sera of adult or newborn animals, or of fetal origin. Fetal bovine serum (FBS) is a cocktail of most of the factors required for cell attachment, growth and proliferation and is thus used as an almost universal growth supplement effective for most types of human and animal (including insect) cells. However, the use of animal serum in cell culture also bears a number of disadvantages. these disadvantages can be seen either from -a theoretical, cell biological point of view, since serum in general is an ill-defined mixture of components in culture media, -from ethical perspectives in terms of animal protection arguments regarding the harvest and collection of FBS from bovine fetuses and -in terms of recent concerns about the global supply versus demand of FBS. As a consequence, a number of strategies were developed to reduce or replace the requirement for FBS in cell culture media. -Furthermore, recent advances in biomedicine, tissue engineering and (adult mesenchymal) stem cell-based therapy demand innovative serum-alternatives of human origin for autologous cell expansion and reimplantation. Also, for the biotechno-

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“…However, chemically-defined serum-free media have not yet been developed for every cell line or primary culture at this time (Bjare, 1992;Even et al, 2006;Gstraunthaler, 2003;Brunner et al, 2010). Additional complicating factors, such as the surface on which the cells are grown and cell preparation, might also have an effect on cell phenotypes and reproducibility.…”

Section: The Serum Marketmentioning

confidence: 99%