Serum-free medium for generation and propagation of functional human cytotoxic and helper T cell clones

Yssel, Hans; Vries, J E de; Koken, Marcel; Blitterswijk, Wim van; Spits, Hergen

doi:10.1016/0022-1759(84)90450-2

Cited by 356 publications

(229 citation statements)

References 9 publications

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“…All cell lines were stored frozen in liquid nitrogen. The T cells were thawed and expanded by stimulation with 5 mg/ml of PHA (Sigma Chemical Co, St. Louis, MO) in the presence of 25 U/ml rIL-2 (Cetus, Emeryville, CA), 10 6 /ml inactivated allogeneic PBMC and 10 5 /ml inactivated JY cells once per three weeks (Yssel et al 1984). Allogeneic PBMC and JY were inactivated by exposure to 50 mg/ml mitomycin c for 45 min, three washes to remove the mitomycin c, and exposure to 2000r g-irradiation.…”

Section: Methodsmentioning

confidence: 99%

Immunotherapy for Visceral Leishmaniasis: Ability of Factors Produced during Anti-leishmania Responses of Skin Test Positive Adults to Inhibit Peripheral Blood Mononuclear Cell Activities Associated with Visceral Leishmaniasis

Holaday

1999

Mem. Inst. Oswaldo Cruz

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Section: Methodsmentioning

confidence: 99%

Immunotherapy for Visceral Leishmaniasis: Ability of Factors Produced during Anti-leishmania Responses of Skin Test Positive Adults to Inhibit Peripheral Blood Mononuclear Cell Activities Associated with Visceral Leishmaniasis

Holaday

1999

Mem. Inst. Oswaldo Cruz

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“…CD3 ϩ CD16 Ϫ NKR ϩ T cells as well as CD3 ϩ CD16 Ϫ NKR Ϫ control T cells were single-cell sorted using a FACStar cell sorter and cyt-clone software and hardware (Becton Dickinson). Sorted T cells were established in culture and maintained as previously described (42). Briefly, T cell clones were cultured in IMDM (Life Technologies, Gaithersburg, MD) containing 200 U/ml rIL-2 (provided by C. Reynolds, National Cancer Institute/Biological Response Modifier Program, Frederick, MD) and 0.1 g/ml of PHA.…”

Section: T Cell Cloningmentioning

confidence: 99%

The Repertoire of Killer Cell Ig-Like Receptor and CD94:NKG2A Receptors in T Cells: Clones Sharing Identical αβ TCR Rearrangement Express Highly Diverse Killer Cell Ig-Like Receptor Patterns

Uhrberg

Valiante

Young

et al. 2001

The Journal of Immunology

118

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Killer cell Ig-like receptor (KIR) and CD94:NKG2A molecules were first defined as human NK cell receptors (NKR), but now are known to be expressed and to function on subpopulations of T cells. Here the repertoires of KIR and CD94:NKG2A expression by T cells from two donors were examined and compared with their previously defined NK cell repertoires. T cell clones generated from peripheral blood of both donors expressed multiple NKR in different combinations and used the range of receptors expressed by NK cells. In both donors αβ T cells less frequently expressed the inhibitory receptors CD94:NKG2A and KIR2DL1 than either γδ T cells or NK cells. In contrast to NK cells, not all NKR+ T cells expressed an inhibitory receptor for autologous HLA class I. This lack of specific inhibitory NKR was especially apparent on αβ T cells of one donor. Overall, αβ T cells exhibited a distinct pattern of NKR expression different from that of γδ T and NK cells, which expressed highly similar NKR repertoires. In one donor, analysis of TCR rearrangement revealed a dominant subset of NKR+ T cells sharing identical TCR α- and β-chains. Remarkably, among 55 T cell clones sharing the same TCRαβ rearrangement 18 different KIR phenotypes were seen, suggesting that KIR expression was initiated subsequently to TCR rearrangement.

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“…Briefly, 29 l of Yssel's medium (Ref. 32; purchased from Diaclone Research), supplemented with 1% human AB ϩ serum, containing different concentrations of human rSDF-1␤, were added to the lower wells of a 96-well ChemoTx-96 disposable chamber with a filter sample site of 3.2 mm diameter and 5-m pore size filters (Neuroprobe, Gaithersburg, MD). Forty thousand T cells, in a volume of 20 l, were transferred directly in triplicate onto the filter sample sites.…”

Section: Chemotaxismentioning

confidence: 99%

Cytokines and Cell Surface Molecules Independently Induce CXCR4 Expression on CD4+ CCR7+ Human Memory T Cells

Jourdan

Vendrell²,

Huguet³

et al. 2000

The Journal of Immunology

Self Cite

107

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In the present study, we show that IL-2, IL-4, IL-7, and IL-15 are able to induce functional CXCR4 surface expression on resting in vitro-generated CD4+ CXCR4− CCR7+ memory T cells. Cytokine-mediated induction of CXCR4 expression was associated with an increase in CXCR4 transcription, enhanced stromal-derived factor-1-induced T cell migration in vitro, and increased susceptibility of these cells to infection with X4 strains of HIV-1. CXCR4 expression could also be induced through an alternative pathway, following coculture of these cells with CD40-activated, autologous, CD34+ progenitor-derived dendritic cells. Although these dendritic cells express transcripts for IL-7 and IL-15, addition of neutralizing anti-IL-7R and IL-15 mAbs did not block induction of CXCR4 expression. Indeed, dendritic cell-mediated up-regulation of CXCR4 expression was found to depend on CD40/CD154 and CD134/CD134L interactions. Whereas activated autologous dendritic cells induced the expression of both CXCR4 and CD25 on a portion of CCR7+ memory T cells, concomitant CD3-mediated activation of these cells further enhanced CD25 expression, but, in contrast, prevented induction of CXCR4 expression. This observation suggests that triggering of the CD134 and CD154 molecules, in contrast to TCR/CD3 complex-mediated stimulation, results in simultaneous T cell activation and CXCR4 expression. Taken together, these results show that common γ-chain-interacting cytokines as well as signals mediated via noncognate interactions between activated dendritic cells and memory T cells are involved in the up-regulation of CXCR4 expression.

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Serum-free medium for generation and propagation of functional human cytotoxic and helper T cell clones

Cited by 356 publications

References 9 publications

Immunotherapy for Visceral Leishmaniasis: Ability of Factors Produced during Anti-leishmania Responses of Skin Test Positive Adults to Inhibit Peripheral Blood Mononuclear Cell Activities Associated with Visceral Leishmaniasis

Immunotherapy for Visceral Leishmaniasis: Ability of Factors Produced during Anti-leishmania Responses of Skin Test Positive Adults to Inhibit Peripheral Blood Mononuclear Cell Activities Associated with Visceral Leishmaniasis

The Repertoire of Killer Cell Ig-Like Receptor and CD94:NKG2A Receptors in T Cells: Clones Sharing Identical αβ TCR Rearrangement Express Highly Diverse Killer Cell Ig-Like Receptor Patterns

Cytokines and Cell Surface Molecules Independently Induce CXCR4 Expression on CD4+ CCR7+ Human Memory T Cells

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