Serum harvested from heifers one month post-zeranol implantation stimulates MCF-7 breast cancer cell growth

Abstract

Abstract. Breast cancer is a serious disease in the uS. numerous risk factors have been linked to this disease. the safety of using growth promoters, such as zeranol, remains under debate due to the lack of sufficient in vitro and in vivo evidence. using celltiter 96™ aqueous non-radioactive cell proliferation assay, real-time pcr and Western blot analysis, we evaluated the effects of sera harvested from experimental and control heifers before and after one month of zeranol implantation on the growth of human breast cancer cell line mcF-7 as well as the involved mechanisms. We found that sera harvested from the heifers following one month of zeranol implantation were more mitogenically potent in stimulating the proliferation of mcF-7 cells when compared to sera harvested from the same heifers before zeranol implantation and the control heifers. Further investigation found that dextran-coated charcoal suppressed the stimulating effect of the sera on mcF-7 cell growth. the mechanisms involved in the mcF-7 cell proliferation stimulated by zeranol-containing sera may include up-regulation of cyclin d1 and down-regulation of p53 and p21 expression at the mrna and protein levels in the cells. the results suggest that the consumption of beef products containing biologically active residues of zeranol or its metabolites is a risk linked to breast cancer development. Further investigation is required in order to clarify this critical issue.

State of the evidence 2017: an update on the connection between breast cancer and the environment

“…Follow-up work demonstrated that ZER is comparable to natural estrogen (estradiol) and the synthetic estrogen diethylstilbestrol (DES) in its ability to transform MCF-10A human breast epithelial cells to a pre-cancerous profile in vitro [ 639 ]. Preliminary data indicate that serum from ZER-treated beef cattle can stimulate the proliferation of normal breast epithelial cells and the transformation of breast tumor cells in vitro [ 640 , 641 ].…”

Algae-meditated route to cuprous oxide (Cu2O) nanoparticle: differential expression profile of MALAT1 and GAS5 LncRNAs and cytotoxic effect in human breast cancer

“…BC in women ranks among the most common malignant tumors; it is also second chief reason of mortality in industrialized countries (Arshi et al 2018). In 2008, the American Cancer Society estimated that in 2014, roughly 182,500 women would be diagnosed with Taherzadeh-Soureshjani and Chehelgerdi Cancer Nano (2020) 11:11 BC and 40,500 women would die because of BC (Ye et al 2010). In Iran, the average age of diagnosis of BC is roughly 15 years lower than the reported cases in western countries (Mousavi et al 2009).…”

Zeranol Down-Regulates p53 Expression in Primary Cultured Human Breast Cancer Epithelial Cells through Epigenetic Modification

“…Our laboratory’s recent experimental results showed that pre-adipocytes isolated from the cattle 60 days post Z-implantation (72 mg/pellets/animal) grow about 12-times faster than the pre-adipocytes isolated from control cattle [ 11 ]. Treatment with 0.2, 1 and 5% Z sera (ZS) harvested from the cattle 30 days post implantation in culture medium significantly stimulated MCF-10A and MCF-7 cell growth [ 12 , 13 ]. We also have evidence showing that leptin, which plays a role in breast cancer development in obesity, induces human breast cancer epithelial cell sensitivity to Z [ 14 ].…”