Serum HSP70

Abstract

Objectives Heat shock protein 70 (HSP70) is overexpressed in human pancreatic cancer cell lines. To determine if serum HSP70 levels are elevated in patients with pancreatic cancer and can function as a biomarker for early detection of pancreatic cancer. Methods Study subjects were divided into 3 groups: histologically proven pancreatic cancer (PC; n = 23), chronic pancreatitis (CP; n = 12), and matched normal control subjects (C; n = 10). Serum HSP70 levels were determined using a novel immunoelectrophoresis method developed and validated by the authors. Significance of difference between the groups was analyzed with analysis of variance (ANOVA). Receiver operating characteristic (ROC) curve analysis was performed to discriminate patients with pancreatic cancer from normal controls. Results The mean ± SE serum HSP70 levels in the PC, CP, and C groups were 1.68 ± 0.083 ng/mL, 0.40 ± 0.057 ng/mL, and 0.04 ng/mL, respectively. Serum HSP70 levels in the PC group were significantly higher compared with either the CP or C groups (P < 0.01). The sensitivity and specificity of elevated serum HSP70 in the PC group was 74% and 90%, respectively. Conclusions Serum HSP70 levels are significantly increased in patients with pancreatic cancer and may be useful as an additional biomarker for the detection of pancreatic cancer.

Extracellular HSP70 Activates ERK1/2, NF-kB and Pro-Inflammatory Gene Transcription Through Binding with RAGE in A549 Human Lung Cancer Cells

“…Its role in the extracellular environment has been shown to be of great relevance, since it has been used as one of the serum biomarkers described for some diseases, such as gastric cancer [8, 36] . Previous studies reported that eHSP70 can bind many receptors present in APCs and the differences in binding affinities between each cell type is probably due to differences in eHSP70 receptors expression [17, 37].…”

“…A wide variety of stressful and biological alterations, including environmental, pathological or physiological stimuli induce a marked increase in intracellular HSP synthesis [3-5]. Many studies show that HSP70 can also be found outside the cell, especially under specific stress conditions [6-8]. It was first believed that origin of extracellular HSP was due to cell necrosis [9], but other studies described the active secretion of HSP70 by alternatives pathways [10-13].…”

AuNPs modified, disposable, ITO based biosensor: Early diagnosis of heat shock protein 70

“…High expression of this chaperone molecule informs about increased tumor grade and poor prognosis as well (Calderwood et al, 2006;Ciocca and Calderwood, 2005;Murphy, 2013). For instance, HSP70 overexpression is a potential marker of early stage of hepatocellular and prostate cancer (Abe et al, 2004;Chuma et al, 2003), breast cancer (Lazaris et al, 1997), lymph node metastasis in colorectal carcinoma (Hwang et al, 2003), bladder urothelial carcinoma (Garg et al, 2010), pancreatic cancer (Dutta et al, 2012). Besides, levels of HSP70 in serum can be correlated to proliferation and tumor size in uterine cervical cancer (Ralhan and Kaur, 1995).…”

Evidence of Multimeric Forms of HSP70 with Phosphorylation on Serine and Tyrosine Residues - Implications for Roles of HSP70 in Detection of GI Cancers

“…In an effort to devise an early detection system, we recently reported evidence to show that plasma soluble heat-shock protein 70 (HSP70) is significantly elevated in pancreatic cancer (Dutta et al, 2012). In this study, we demonstrated a novel approach involving the immunocapture of plasma HSP70 and the subsequent demonstration by Western blot of the existence of several isoforms of phosphorylated HSP70 on serine and tyrosine residues.…”