Serum IL8 and mRNA level of CD11b in circulating neutrophils are increased in clinically amyopathic dermatomyositis with active interstitial lung disease

Abstract

The objective of this study is to assess serum IL8 and the potential activity of circulating neutrophils on relative messenger RNA (mRNA) levels and their relationship with disease activity in clinically amyopathic dermatomyositis (CADM) associated with interstitial lung disease (ILD). We studied 18 CADM patients and compared them with 18 classic dermatomyositis (DM) patients and 18 healthy control subjects. Serum IL8 level and mRNA expressions of neutrophils (chemokine (C-X-C motif) receptor 1 (CXCR1), cluster of differentiation molecule 11b (CD11b), cluster of differentiation 64 (CD64), myeloid cell leukemia 1 (MCL1), interleukin-18 (IL18)) were detected. The overproduction of serum IL8 level was most significant in the CADM group with active period. The mRNA expressions of CD11b, IL18, and MCL1 were greatly increased in the neutrophils in patients with CADM compared with DM or healthy controls. Up-expressions of CD11b, IL18, and MCL1 were detected in the neutrophils in CADM patients of active period compared with remission period. A positive correlation was found between CD11b mRNA level and high-resolution computed tomography (HRCT) score, in CADM associated with ILD. Serum IL8 level and mRNA levels of CD11b, MCL1, and IL18 in circulating neutrophils are related with the disease activity of CADM-ILD. The mRNA level of CD11b is positively correlated with HRCT score in CADM-ILD.

Characteristic Cytokine and Chemokine Profiles in Encephalitis of Infectious, Immune-Mediated, and Unknown Aetiology

“…In CSF there were many more differences in the concentrations of mediators between the aetiological groups. Specifically, higher concentrations of IL-8 were identified in both the infectious and unknown group as opposed to immune-mediated cases, which would be consistent with the key finding of the role of neutrophils in our study, given the important role of IL-8 in neutrophil activation [ 47 – 49 ]. In addition, greater concentrations of IL-1RA, IL-4, IL-10 and IL-1β were found in the immune-mediated group.…”

Biomarkers in Adult Dermatomyositis: Tools to Help the Diagnosis and Predict the Clinical Outcome

“…Concerning interstitial lung disease (ILD), Gono et al [48] and Yang et al [49] found that IL-18 was higher in DM-associated ILD (DM-ILD) compared to DM without ILD but no difference was found between RP-ILD compared to non-RP-ILD [23, 51]. Zou et al [52] confirmed these results in CADM neutrophils: they found an increase of IL-18 mRNA in CADM patients compared to classical DM (cDM) and HD.…”

“…CXCL8 was found elevated in DM and CADM associated with anti-MDA5 or anti-ARS Ab compared to HD in one study [62]. CXCL8 seems to be implicated in CADM and ILD since serum level was increased in CADM compared to that in classical DM with ILD and HD [52] and in RP-ILD but not in C-ILD compared to non-ILD [65] or in ILD compared to non-ILD in anti-MDA5 or anti-ARS-Ab-positive patients [62]; however, in this last study, in multiple linear regression analysis CXCL8 was not associated with ILD. A positive correlation was found between CXCL8 and MITAX [62], global VAS, pulmonary VAS, and serum ferritin level [65].…”

Therapeutic plasma exchange for clinically amyopathic dermatomyositis (CADM) associated with rapidly progressive interstitial pneumonia

“…In addition, we did not evaluate changes to other inflammatory cytokines, including serum interleukin (IL)-6, IL-8, IL-10, IL-18, interferon (IFN)-α, IFN-γ, macrophage colony-stimulating factor, CX3CL1, or messenger RNA levels of CD11b in circulating neutrophils, which have also been indicated to represent pathogenic factors for RPIP. [20][21][22][23][24][25] Activation of monocytes and macrophages through these factors has been suggested to underlie the pathophysiology of anti-MDA5 Ab-positive DM patients. 20,25 Further studies should be conducted to elucidate whether removal of these factors with TPE contributes to disease control.…”