Serum laminin and basic fibroblast growth factor concentrations in patients with complicatedPlasmodium falciparum malaria

Burgmann, Heinz; Looareesuwan, S; Viravan, C; Vanijanonta, S; Zedwitz-Liebenstein, Konstantin; Vorbach, H.; Graninger, Wolfgang

doi:10.1007/bf01541393

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…Third, serum in the DRG explant media may have an effect on neurite orientation along the fibers. Serum contains both proteins that potentiate neurite outgrowth, including IGF-I [19], laminin [45] and fibronectin [21], as well as non-adhesive proteins, such as serum albumin, that likely reduce neurite-to-fiber adhesion [46]. Therefore, serum could decrease neurite alignment by simultaneously potentiating rapid neurite growth on less adhesive nanofibers, encouraging neurites to jump from one nanofiber to another.…”

Section: Discussionmentioning

confidence: 99%

The design of electrospun PLLA nanofiber scaffolds compatible with serum-free growth of primary motor and sensory neurons

et al. 2008

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Aligned electrospun nanofibers direct neurite growth and may prove effective for repair throughout the nervous system. Applying nanofiber scaffolds to different nervous system regions will require prior in vitro testing of scaffold designs with specific neuronal and glial cell types. This would be best accomplished using primary neurons in serum-free media; however, such growth on nanofiber substrates has not yet been achieved. Here we report the development of poly(L-lactic acid) (PLLA) nanofiber substrates that support serum-free growth of primary motor and sensory neurons at low plating densities. In our study, we first compared materials used to anchor fibers to glass to keep cells submerged and maintain fiber alignment. We found that poly(lactic-co-glycolic acid) (PLGA) anchors fibers to glass and is less toxic to primary neurons than bandage and glue used in other studies. We then designed a substrate produced by electrospinning PLLA nanofibers directly on cover slips pre-coated with PLGA. This substrate retains fiber alignment even when the fiber bundle detaches from the cover slip and keeps cells in the same focal plane. To see if increasing wettability improves motor neuron survival, some fibers were plasma etched before cell plating. Survival on etched fibers was reduced at the lower plating density. Finally, the alignment of neurons grown on this substrate was equal to nanofiber alignment and surpassed the alignment of neurites from explants tested in a previous study. This substrate should facilitate investigating the behavior of many neuronal types on electrospun fibers in serum-free conditions.

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Section: Discussionmentioning

confidence: 99%

The design of electrospun PLLA nanofiber scaffolds compatible with serum-free growth of primary motor and sensory neurons

et al. 2008

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“…LAMB2 is an extracellular high-affinity receptor and is associated with GO term “substrate adhesion-dependent cell spreading” to which infected erythrocytes (IE) have been reported to bind, along with other adhesion molecules such as ICAM-1 and vascular cell adhesion molecule (VECAM). Burgmann et al ( 74 ) and Wenisch et al ( 75 ) found that laminin levels in serum were increased in severe malaria. Mahamar et al ( 76 ) later contradicted this, finding that the binding of infected erythrocytes to endothelial receptors, including laminin, was the same in severe and nonsevere malaria.…”

Section: Resultsmentioning

confidence: 99%

Dual RNA Sequencing Meta-analysis in Plasmodium Infection Identifies Host-Parasite Interactions

2021

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Dual RNA sequencing (RNA-Seq) is the simultaneous transcriptomic analysis of interacting symbionts, for example, in malaria. Potential cross-species interactions identified by correlated gene expression might highlight interlinked signaling, metabolic, or gene regulatory pathways in addition to physically interacting proteins. Often, malaria studies address one of the interacting organisms—host or parasite—rendering the other “contamination.” Here we perform a meta-analysis using such studies for cross-species expression analysis. We screened experiments for gene expression from host and Plasmodium. Out of 171 studies in Homo sapiens, Macaca mulatta, and Mus musculus, we identified 63 potential studies containing host and parasite data. While 16 studies (1,950 samples) explicitly performed dual RNA-Seq, 47 (1,398 samples) originally focused on one organism. We found 915 experimental replicates from 20 blood studies to be suitable for coexpression analysis and used orthologs for meta-analysis across different host-parasite systems. Centrality metrics from the derived gene expression networks correlated with gene essentiality in the parasites. We found indications of host immune response to elements of the Plasmodium protein degradation system, an antimalarial drug target. We identified well-studied immune responses in the host with our coexpression networks, as our approach recovers known broad processes interlinked between hosts and parasites in addition to individual host and parasite protein associations. The set of core interactions represents commonalities between human malaria and its model systems for prioritization in laboratory experiments. Our approach might also allow insights into the transferability of model systems for different pathways in malaria studies. IMPORTANCE Malaria still causes about 400,000 deaths a year and is one of the most studied infectious diseases. The disease is studied in mice and monkeys as lab models to derive potential therapeutic intervention in human malaria. Interactions between Plasmodium spp. and its hosts are either conserved across different host-parasite systems or idiosyncratic to those systems. Here we use correlation of gene expression from different RNA-Seq studies to infer common host-parasite interactions across human, mouse, and monkey studies. First, we find a set of very conserved interactors, worth further scrutiny in focused laboratory experiments. Second, this work might help assess to which extent experiments and knowledge on different pathways can be transferred from models to humans for potential therapy.

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“…The selection of candidate novel receptors for the study was based on the following observations: laminin plasma levels are increased during acute malaria infection, suggesting vascular injury that may expose extracellular matrix molecules (ECM) like laminin and possibly other ECM like cellular fibronectin that can then mediate IE adhesion 26 , 27 . Further, it has been reported that laboratory parasite line FCR3 binds cellular fibronectin 28 .…”

Section: Resultsmentioning

confidence: 99%

Host factors that modify Plasmodium falciparum adhesion to endothelial receptors

Mahamar¹,

Attaher²,

Swihart

et al. 2017

Sci Rep

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P. falciparum virulence is related to adhesion and sequestration of infected erythrocytes (IE) in deep vascular beds, but the endothelial receptors involved in severe malaria remain unclear. In the largest ever study of clinical isolates, we surveyed adhesion of freshly collected IE from children under 5 years of age in Mali to identify novel vascular receptors, and examined the effects of host age, hemoglobin type, blood group and severe malaria on levels of IE adhesion to a panel of endothelial receptors. Several novel molecules, including integrin α3β1, VE-cadherin, ICAM-2, junctional adhesion molecule-B (JAM-B), laminin, and cellular fibronectin, supported binding of IE from children. Severe malaria was not significantly associated with levels of IE adhesion to any of the 19 receptors. Hemoglobin AC, which reduces severe malaria risk, reduced IE binding to the receptors CD36 and integrin α5β1, while hemoglobin AS did not modify IE adhesion to any receptors. Blood groups A, AB and B significantly reduced IE binding to ICAM-1. Severe malaria risk varies with age, but age significantly impacted the level of IE binding to only a few receptors: IE binding to JAM-B decreased with age, while binding to CD36 and integrin α5β1 significantly increased with age.

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Serum laminin and basic fibroblast growth factor concentrations in patients with complicatedPlasmodium falciparum malaria

Cited by 5 publications

References 23 publications

The design of electrospun PLLA nanofiber scaffolds compatible with serum-free growth of primary motor and sensory neurons

The design of electrospun PLLA nanofiber scaffolds compatible with serum-free growth of primary motor and sensory neurons

Dual RNA Sequencing Meta-analysis in Plasmodium Infection Identifies Host-Parasite Interactions

Host factors that modify Plasmodium falciparum adhesion to endothelial receptors

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