Serum Level of Long Noncoding RNA B3GALT5-AS1 as a Diagnostic Biomarker of Colorectal Cancer

Ding, Ye; Wei, Feng; Ge, Jian-Kang; Dai, Lu; Liu, Tingting; Hua, Xinyi; Ju, Shaoqing; Yu, Juan

doi:10.2217/fon-2019-0820

Cited by 11 publications

(4 citation statements)

References 22 publications

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“…In addition, increasing serum B3GALT5-AS1 level was related to histological differentiation and TNM stage. Therefore, B3GALT5-AS1 might be a novel potential diagnostic biomarker for CRC (Ding et al, 2020). Our preliminary experiment confirmed that Linc01836 was significantly higher in the serum of CRC than in healthy folks.…”

Section: Discussionsupporting

confidence: 68%

Upregulated Linc01836 in Serum Promisingly Serving as a Diagnostic and Prognostic Biomarker for Colorectal Cancer

et al. 2022

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Objectives: Colorectal cancer (CRC) is a common carcinoma of the gastrointestinal tract with high incidence and mortality worldwide. Studies have shown that long noncoding RNAs (lncRNAs) play important roles in CRC. Our purpose is to investigate the potential of serum Linc01836 as a diagnostic and prognostic marker in CRC.Methods: We evaluated the expression of Linc01836 via quantitative real-time polymerase chain reaction (qRT-PCR). The serum CEA, CA19-9, Cyfra21-1, and CA72-4 concentrations were measured by Architect I4000 SR. Receiver operating characteristic (ROC) curves were plotted to estimate the diagnostic value in CRC. Relationship between serum Linc01836 expression and clinicopathological characteristics of CRC cases was analyzed via chi-square test. The underlying mechanism of Linc01836 on the development and prognosis in CRC was predicted by bioinformatic analysis.Results: The method of qRT-PCR for Linc01836 detection was confirmed with high precision and specificity. Serum Linc01836 expression in CRC patients was significantly higher than that in healthy donors (p < 0.0001) and benign patients (p < 0.0001), and declined after resection (p < 0.01). High expression of Linc01836 was associated with histological stage (p = 0.002) and lymph node metastasis (p = 0.006). In addition, serum Linc01836 could effectively differentiate CRC patients from the healthy folks, with favorable area under the curve (AUC) of 0.809 (95% CI: 0.757–0.861, p < 0.001). What is more, the combination of serum Linc01836, CEA, and Cyfra21-1 could improve diagnostic sensitivity (92.0%). Linc01836 was averagely located in the nucleus and cytoplasm, suggesting that it might participate in CRC progression and prognosis through the crosstalk among lncRNAs, miRNAs, and mRNAs.Conclusion: Linc01836 may serve as a valuable noninvasive biomarker for population screening, early detection, and clinical surveillance of CRC.

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Section: Discussionsupporting

confidence: 68%

Upregulated Linc01836 in Serum Promisingly Serving as a Diagnostic and Prognostic Biomarker for Colorectal Cancer

et al. 2022

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“… 233 Recently, B3GALT5‐AS1 has been reported to be related to TNM stage and histological differentiation. 234 There are also other lncRNA that regulated CRLM through ceRNA mode, for example MALAT1, SNHG14, SNHG7, H19, and LINC00485. Taken together, these lncRNA could be a therapeutic target and promising biomarkers for prognosis prediction of CRLM.…”

Section: Mechanisms/pathophysiologymentioning

confidence: 99%

Colorectal liver metastasis: molecular mechanism and interventional therapy

Zhou

Liu

Wang

et al. 2022

Sig Transduct Target Ther

142

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Colorectal cancer (CRC) is one of the most frequently occurring malignancy tumors with a high morbidity additionally, CRC patients may develop liver metastasis, which is the major cause of death. Despite significant advances in diagnostic and therapeutic techniques, the survival rate of colorectal liver metastasis (CRLM) patients remains very low. CRLM, as a complex cascade reaction process involving multiple factors and procedures, has complex and diverse molecular mechanisms. In this review, we summarize the mechanisms/pathophysiology, diagnosis, treatment of CRLM. We also focus on an overview of the recent advances in understanding the molecular basis of CRLM with a special emphasis on tumor microenvironment and promise of newer targeted therapies for CRLM, further improving the prognosis of CRLM patients.

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“…Multiple lncRNAs were abnormally expressed, thereby acting as vital modulators in the regulation of progression of various cancers including in CRC [ 6 , 7 ]. High-expressed lncRNA HOTAIR is responsible for the pathogenesis of aggressive colon cancer [ 8 ] and gastrointestinal stromal tumor [ 9 ]; NR2F2-AS1 with a high expression is predictive of a lower overall survival of patients with CRC [ 10 ]; serum B3GALT5-AS1 is low-expressed in CRC patients, which therefore could be regarded as a diagnostic biomarker for identifying CRC patients [ 11 ]. Previous research based on bioinformatics found 5 lncRNAs (AL117190.1, MEG3, COL4A2-AS1, LINC00184, and MIR22 HG) as vital prognostic factors for breast cancer patients [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA COL4A2-AS1 facilitates cell proliferation and glycolysis of colorectal cancer cells via miR-20b-5p/hypoxia inducible factor 1 alpha subunit axis

Wang

Jing

et al. 2021

Bioengineered

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Long non-coding RNAs (lncRNAs) have critical functions in tumorigenesis and progression of colorectal cancer (CRC). The role of lncRNA COL4A2-AS1 (COL4A2-AS1) lacks system investigation. The current study comprehensively analyzed the expression, biological functions, and mechanism of COL4A2-AS1 in CRC through performing real-time quantitative PCR (RT-qPCR), Western blot, cell transfection, cell colony assay, MTT assay, flow cytometry and dual-luciferase reporter system assays. A xenograft model of CRC was constructed to further verify the function of COL4A2-AS1 in CRC progression in vivo. The data revealed an upregulated expression of COL4A2-AS1 in CRC tissues and cell lines than paired adjacent tissues and normal cell line. Silencing COL4A2-AS1 inhibited proliferation, aerobic glycolysis, and promoted apoptosis of CRC cells in vivo and in vitro. However, overexpression of COL4A2-AS1 significantly promoted CRC cell proliferation and aerobic glycolysis. In CRC cells, miR-20b-5p was sponged by COL4A2-AS1 and hypoxia-inducible factor 1 alpha subunit (HIF1A). Restoration of HIF1A expression reversed the inhibitory effects of silencing COL4A2-AS1 on aerobic glycolysis and proliferation of CRC cells. The current findings showed that COL4A2-AS1 promoted the proliferation, and aerobic glycolysis of CRC cells potentially through modulating the miR-20b-5p/HIF1A axis.

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Serum Level of Long Noncoding RNA B3GALT5-AS1 as a Diagnostic Biomarker of Colorectal Cancer

Cited by 11 publications

References 22 publications

Upregulated Linc01836 in Serum Promisingly Serving as a Diagnostic and Prognostic Biomarker for Colorectal Cancer

Upregulated Linc01836 in Serum Promisingly Serving as a Diagnostic and Prognostic Biomarker for Colorectal Cancer

Colorectal liver metastasis: molecular mechanism and interventional therapy

Long non-coding RNA COL4A2-AS1 facilitates cell proliferation and glycolysis of colorectal cancer cells via miR-20b-5p/hypoxia inducible factor 1 alpha subunit axis

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