Serum lipid abnormalities are not associated with apoB 3' VNTR polymorphism in nephrotic children

Abstract

ABSTRACT. Apolipoprotein B (apoB) gene 3' variable number of tandem repeat (VNTR) is highly variable, and therefore can be an informative marker for associative analysis of lipid metabolism. This is the first report focusing on a possible association of apoB VNTR polymorphism with nephrotic hyperlipidemia. Genomic DNA was extracted from 500 children with primary nephrotic syndrome (PNS) and 500 healthy controls. The apoB genotype was determined by PCR analysis. Allele size distribution followed a unimodal curve, with the main peak at the hypervariable element 35 (HVE35); the most prevalent genotype was HVE35/35 in both control and PNS children. The genotype and allele distributions of apoB variants in PNS children were not significantly different from controls. There was significant variation in serum lipid profiles among different genotypes in control children. Individuals with the long (L) allele exhibited significantly higher total cholesterol, low-density lipoprotein cholesterol (LDL-C) and apoB levels than those with the medium (M) or short (S) allele; consequently, M/L carriers had significantly higher total cholesterol, LDL-C and apoB concentrations than did S/S, S/M, S/L, or M/M carriers. However, in PNS children, no significant differences in serum lipid levels were observed among individuals with different genotypes and alleles of apoB 3' VNTR. We conclude that hyperlipidemia in nephrotic children is not associated with apoB 3' VNTR polymorphism.

Gene polymorphism of 3'APO-VNTR in Egyptians with coronary artery disease

“…The difference might be referred to the population ethnicity or the disease. In China, Hu et al [25] reported that the genotype and allele distributions of ApoB variants in Chinese children with primary nephrotic syndrome were not significantly different from the control volunteers.…”

Relationship between the Apolipoprotein AI, B gene polymorphism and the risk of non-traumatic osteonecrosis

“…The apoB gene polymorphism can affect the plasma TC, LDL and ApoB levels [ 23 ]. Previous studies indicated that Xba I, EcoR I and 3′-VNTR in ApoB gene were associated with lipid metabolism and related diseases [ 24 , 25 ]. However, in the present study, we did not found association of these SNPs in ApoB gene and osteonecrosis.…”