Serum Neuroglobin as a Potential Prognostic Biomarker for Cognitive Impairment After Intracerebral Hemorrhage

Abstract

ObjectiveStroke is closely related to dementia, but there are few prospective studies on cognitive decline after stroke in patients with cerebral hemorrhage. Neuroglobin is an oxygen-binding protein mainly expressed in brain neurons. The aim of our current study was to determine whether neuroglobin could serve as a biomarker for cognitive prognosis in patients with intracerebral hemorrhage (ICH).MethodsThree hundred and sixteen patients with ICH were consecutively enrolled in a prospective study. Baseline data such as age and gender of ICH patients on admission were recorded. Serum neuroglobin concentrations were determined by enzyme-linked immunosorbent assay (ELISA). All ICH patients 3 months after onset were divided into post-stroke cognitive impairment group (PSCI) and non-PSCI group according to MoCA assessment results.ResultsThe PSCI and Non-PSCI groups had serum neuroglobin concentrations of (4.7 ± 0.9) and (7.5 ± 1.1) ng/ml, respectively, with a statistically significant difference between the two groups (p < 0.05). Age, gender, LDL, FBG, SBP, DBP, NHISS, and Hematoma volume were found to be adversely connected with MoCA (p < 0.05), while education, HDL, and serum neuroglobin were found to be positively correlated with MoCA (p < 0.05). After controlling for baseline data, regression analysis revealed that serum neuroglobin was remained an efficient biomarker for predicting cognitive performance in individuals with ICH (p < 0.05). The diagnostic accuracy of blood neuroglobin concentration for PSCI in ICH patients was 72.6%, the sensitivity was 67.4%, and the specificity was 75.5%, according to receiver operating characteristic (ROC) curve analysis.ConclusionsSerum neuroglobin may serve as a potential biomarker to predict cognitive decline after ICH.

Post-stroke cognitive impairment: exploring molecular mechanisms and omics biomarkers for early identification and intervention

“…The proteins ARTN, HGF ( Gallo et al, 2015 ), and VEGF ( Lee et al, 2010 ) are associated with angiogenesis and neuroprotection, and their early predictive role in PSCI patients was identified ( Prodjohardjono et al, 2020 ; Hazelwood et al, 2022 ). Both neuroglobin and hemoglobin, which are subtypes of pearl proteins ( Lopez et al, 2010 ; Baez et al, 2016 ), play an important role in oxidative stress and protect brain tissue from hypoxic and ischemic damage, so their reduced levels increase the risk of developing PSCI ( Gao et al, 2022 ; Li Z. et al, 2022 ).…”

“…It is worth noting that only a small number of studies have demonstrated the diagnostic performance of these markers and their causal relationship with PSCI, such as neuroglobin ( Gao et al, 2022 ), serum amyloid A ( Zhang Y. et al, 2021 ), L-carnitine ( Che et al, 2022 ) and TMAO ( Zhu et al, 2020 ). The rest of the biomarkers have only shown associations with PSCI at a statistical level, and these changes may be related to multiple pathways including inflammation, so subsequent studies need to be approached with caution.…”

Neuroglobin protects against cerebral ischemia/reperfusion injury in rats by suppressing mitochondrial dysfunction and endoplasmic reticulum stress‐mediated neuronal apoptosis through synaptotagmin‐1

“…36 Ngb exhibits a high affinity for oxygen, represents a potent free radical scavenger, and exerts protective effects on the brain after hypoxia and ischemia, with the characteristic of being predominantly expressed in brain neurons. 37,38 This paper elucidated that Ngb could bind to Syt1 protein and upregulate its expression to suppress mitochondrial dysfunction and ERS-mediated neuronal apoptosis, thereby protecting against cerebral I/R injury.…”

Kadar Neuroglobin dan Sitoglobin dalam Plasma, Cairan Serebro Spinalis, dan Jaringan Otak Pasien Strok Hemoragik

“…Penelitian lain oleh Gao Y, yang melakukan pengukuran Ngb pada serum pasien post stroke. Hasil penelitiannya menyimpulkan bahwa Ngb serum dapat berfungsi sebagai biomarker yang potensial untuk memprediksi penurunan kognitif setelah ICH (27). Penelitian ini juga dapat mendeteksi dan mengukur Ngb di CSS, studi lain yang juga telah mendeteksi dan mengukur protein Ngb di dalam CSS adalah penelitian Casado B dkk., namun bukan pada pasien strok hemoragik melainkan pada subyek yang mengalami nyeri (28).…”