Serum Osteopontin Concentrations in Relation to Coronary Artery Disease

Mohamadpour, Amir Hooshang; Abdolrahmani, Leila; Mirzaei, Hamed; Sahebkar, Amirhosein; Moohebati, Mohsen; Gholami, Maryam; Ghayour‐Mobarhan, Majid

doi:10.1016/j.arcmed.2015.02.005

Cited by 31 publications

(23 citation statements)

References 32 publications

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“…Thus, OPN has been suggested to be implicated in vascular injury responses by increasing extracellular matrix invasion, migration and proliferation of VSMCs [12–14]. Furthermore, OPN was reported to be strongly expressed in a synthetic VSMC phenotype [15], and suggested to be a key factor of the development of vascular remodeling diseases [16,17]. Although the vascular remodeling effects of OPN have aroused considerable research interest [18], little is known of its role in vascular wall remodeling.…”

Section: Introductionmentioning

confidence: 99%

α-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression

et al. 2017

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α-Iso-cubebene (ICB) is a dibenzocyclooctadiene lignin contained in Schisandra chinensis (SC), a well-known medicinal herb that ameliorates cardiovascular symptoms. Thus, we examined the effect of ICB on vascular smooth muscle cell (VSMC) proliferation, a key feature of diverse vascular diseases. When VSMCs primary cultured from rat thoracic aorta were stimulated with PDGF (1–10 ng/ml), cell proliferation and osteopontin (OPN) expression were concomitantly up-regulated, but these effects were attenuated when cells were treated with MPIIIB10, a neutralizing monoclonal antibody for OPN. In aortic tissues exposed to PDGF, sprouting VSMC numbers increased, which was attenuated in tissues from OPN-deficient mice. Furthermore, VSMC proliferation and OPN expression induced by PDGF were attenuated dose-dependently by ICB (10 or 30 μg/ml). Reporter assays conducted using OPN promoter-luciferase constructs showed that the promoter region 538–234 bp of the transcription start site was responsible for transcriptional activity enhancement by PDGF, which was significantly inhibited by ICB. Putative binding sites for AP-1 and C/EBPβ in the indicated promoter region were suggested by TF Search, and increased binding of AP-1 and C/EBPβ in PDGF-treated VSMCs was demonstrated using a ChIP assay. The increased bindings of AP-1 and C/EBPβ into OPN promoter were attenuated by ICB. Moreover, the PDGF-induced expression of OPN was markedly attenuated in VSMCs transfected with siRNA for AP-1 and C/EBPβ. These results indicate that ICB inhibit VSMC proliferation by inhibiting the AP-1 and C/EBPβ signaling pathways and thus downregulating OPN expression.

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Section: Introductionmentioning

confidence: 99%

α-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression

et al. 2017

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“…MiRNAs have central roles in many biological cellular and molecular pathways Mohamadpour et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA: Relevance to stroke diagnosis, prognosis, and therapy

Mirzaei

Momeni

Saadatpour

et al. 2017

Journal Cellular Physiology

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165

140

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Stroke is a life-threatening disease that accounts for a considerable burden of mortality in both developing and developed world. Identification of specific biomarkers for stroke and its outcomes can greatly contribute to improved care of patients. MicroRNAs (miRNAs) are known as novel biomarkers that could be used as diagnostic, prognostic, and therapeutic biomarkers. Various studies have shown that miRNAs have key roles in the pathogenesis of stroke, and its complications and outcomes. In addition, there is evidence showing that mesenchaymal stromal cell-derived exosomes containing miRNAs can be used for monitoring and treatment of various diseases such as stroke. Here, we summarized various aspects of miRNA applications in different stages of stroke.

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“…This is followed by lipid-laden macrophage (foam cell) accumulation in the subendothelial space of damaged endothelium within the vascular wall of medium-and/or small-sized arteries (10). OPN is found at the site of atherosclerotic lesions, especially in association with macrophages and foam cells, suggesting that OPN plays an important role in the development and progression of atherosclerosis, vascular remodeling, and restenosis (11). OPN levels are associated with atherosclerosis and coronary artery disease, and in these plaques, serve as a chemotactic agent for infiltrating macrophages (12).…”

Section: Introductionmentioning

confidence: 99%

Role of Osteopontin in Systemic Lupus Erythematosus

Maghraby¹,

Refaei²,

Hassan³

et al. 2018

IAM

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Background: Systemic lupus erythematosus (SLE) is a multisystemic disease, caused by a variety of factors, which lead to immunological abnormalities. Osteopontin (OPN) is a pleiotropic protein, important in bone remodeling and immune system signaling. It produced by various cells, including immune cells, and played a key role in regulating T-helper 1/T-helper 2 balances, stimulating B lymphocytes to produce antibodies, regulating macrophages, neutrophils and inducing dendritic cells. Over expression of OPN has been associated with the pathogenesis of immune-mediated diseases. Objectives: To investigate the role of osteopontin in SLE and to assess its relation to disease activity and subclinical atherosclerosis. Subjects and Methods: This cross sectional case control study was conducted on 30 SLE patients fulfilling SLICCA /ACR criteria for SLE classification and 30 subjects sex-and agematched apparently healthy as control group. All patients were assessed for SLE disease activity by the systemic lupus activity Index (SLEDAI). Serum OSP and Doppler ultrasound for carotid artery media thickness (CIMT) were measured in all patients and control. Results: Plasma OPN levels were significantly higher in SLE patients than in control group (P<0.001) and had significant positive correlation with disease activity (r 0,595, P<0.05) and CIMT (r = 0.400, P <0.001), but there was no significant correlation between CIMT and disease activity. Conclusion: serum level of OPN was higher in SLE patients and correlated with disease activity and CIMT, and can be used as a marker of subclinical atherosclerosis.

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Serum Osteopontin Concentrations in Relation to Coronary Artery Disease

Cited by 31 publications

References 32 publications

α-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression

α-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression

MicroRNA: Relevance to stroke diagnosis, prognosis, and therapy

Role of Osteopontin in Systemic Lupus Erythematosus

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