Serum Oxylipin Profiles Identify Potential Biomarkers in Patients with Acute Aortic Dissection

Abstract

Aortic dissection (AD) is a life-threatening cardiovascular disease with a dismal prognosis. Inflammation plays an important role in AD. Oxylipins are bioactive lipids involved in the modulation of inflammation and may be involved in the pathogenesis and progression of AD. This study aims to identify possible metabolites related to AD. A total of 10 type A Aortic dissection (TAAD) patients, 10 type B Aortic dissection (TBAD) patients and 10 healthy controls were included in this study. Over 100 oxylipin species were identified and quantified by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis. Our investigation demonstrated substantial alterations in 91 oxylipins between AD and healthy individuals. Patients with TAAD had 89 entries accessible compared to healthy controls. According to orthogonal partial least squares discriminant analysis (OPLS-DA), fitness (R2X = 0.362 and R2Y = 0.807, p = 0.03) and predictability (Q2 = 0.517, p = 0.005) are the validation parameters between the two groups. Using multivariate logistic regression, 13-HOTrE and 16(17)-EpDPE were the risk factors in the aortic patients group compared to healthy people (OR = 2.467, 95%CI:1.256–7.245, p = 0.035; OR = 0.015, 95%CI:0.0002–0.3240, p = 0.016, respectively). In KEGG enrichment of differential metabolites, the arachidonic acid metabolism pathway has the most metabolites involved. We established a diagnostic model in distinguishing between AD and healthy people. The AUC was 0.905. Oxylipins were significantly altered in AD patients, suggesting oxylipin profile is expected to exploit a novel, non-invasive, objective diagnosis for AD.

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

“…Since oxylipins modulate the inflammatory response, they are involved in the pathogenesis of various inflammatory diseases. In one of them, aortic dissection, serum metabolic profiling revealed 91 altered oxylipins [ 56 ]. Subsequent pathway analysis indicated that most metabolites were part of the arachidonic acid metabolism pathway.…”

“…Being an inflammation disease, acute aortic dissection is influenced by oxylipins, which modulate inflammation responses, as was noted earlier. According to recent studies, the levels of serum oxylipins in patients with acute aortic dissection were significantly altered [ 56 ].…”

“…The proposed method includes the profiling of 19 main metabolites, which were chosen in accordance with their biological relevance to HF. Modern studies with metabolic profiling of cardiovascular diseases utilize the following MS methods: LC-MS/MS [ 56 ], UPLC/Q-TOF [ 299 ], and GC-MS/MS [ 58 ].…”