Serum polysialylated neural cell adhesion molecule in childhood neuroblastoma

Glüer, S.; Schelp, C; Madry, Norbert; Schweinitz, Dietrich von; Eckhardt, Matthias; Gerardy‐Schahn, Rita

doi:10.1038/bjc.1998.450

Cited by 46 publications

(32 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, in colon carcinoma, pancreatic cancer, and astrocytoma, NCAM expression is markedly down-regulated, and the loss of NCAM correlates with poor prognosis (8 -11). In contrast, in neuroblastoma and certain neuroendocrine tumors, cancer progression correlates with increased NCAM expression and extensive polysialylation (12)(13)(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Loss of Neural Cell Adhesion Molecule Induces Tumor Metastasis by Up-regulating Lymphangiogenesis

et al. 2004

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Loss of Neural Cell Adhesion Molecule Induces Tumor Metastasis by Up-regulating Lymphangiogenesis

et al. 2004

View full text Add to dashboard Cite

show abstract

“…In neuroblastoma expression of polySia-NCAM was highest in patients with advanced stage of disease and correlated with other prognostic markers, such as MYCN amplification (15). Furthermore serum levels of polySia-NCAM were shown to correlate with tumour content and were found to decrease during successful therapy (16). The key enzymes in the biosynthesis of polySia are the two closely related polysialyltransferases ST8SiaII and ST8SiaIV, formerly named STx and PST, respectively (17).…”

Section: Introductionmentioning

confidence: 99%

Expression of the neural cell adhesion molecule and polysialic acid in human neuroblastoma cell lines

Valentiner

Mühlenhoff²,

Lehmann³

et al. 2011

Int J Oncol

View full text Add to dashboard Cite

Abstract. The neural cell adhesion molecule NCAM is a cell surface glycoprotein of the immunoglobulin superfamily and is widely expressed in tumours of neuroectodermal origin such as neuroblastoma. NCAM can be decorated by the carbohydrate polymer polysialic acid (polySia), which attenuates NCAM-mediated cell adhesion and increases cellular motility. The key enzymes in the biosynthesis of polySia are the two polysialyltransferases ST8SiaII and ST8SiaIV. In the present study, expression of NCAM, polySia-NCAM, ST8SiaII and ST8SiaIV was investigated in five human neuroblastoma cell lines before and after xenografting into SCID mice by immunohistochemistry, Western blot analysis and real-time PCR. Results were correlated with the metastatic potential. In vitro, three cell lines (LAN-1, LAN-5 and SH-SY5Y) were positive for polySia attached to the transmembrane isoforms NCAM-140 and NCAM-180, whereas Kelly and SK-N-SH cells were negative for NCAM and polySia. In the presence of NCAM, the level of polySia correlated with the amount of polysialyltransferase transcripts, which were highest in LAN-1, LAN-5 and SH-SY5Y cells. In the respective primary tumours grown in SCID mice, the expression patterns of NCAM, polySia and polysialyltransferases were similar to those observed in vitro. After subcutaneous engraftment, polySia-NCAM-positive neuroblastoma developed disseminated micrometastases, a metastatic pattern that was not observed for tumours derived from NCAM-negative cell lines. Together, this indicates that the presence of polySia reduces the adhesiveness of tumour cells and promotes dissemination.

show abstract

“…As an oncodevelopmental antigen, PSA is reexpressed during progression of several malignant human tumors, including small cell lung carcinoma, Wilms' tumor, neuroblastoma, and rhabdomyosarcoma (11,14,17). In these tumors, polysialylation of NCAM appears to increase the metastatic potential and has been correlated with tumor progression and a poor prognosis (7,8,14,16,17,48).…”

mentioning

confidence: 99%

Polysialic Acid Directs Tumor Cell Growth by Controlling Heterophilic Neural Cell Adhesion Molecule Interactions

Seidenfaden

Krauter

Schertzinger

et al. 2003

Molecular and Cellular Biology

176

134

View full text Add to dashboard Cite

Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), promotes neural plasticity and tumor malignancy, but its mode of action is controversial. Here we establish that PSA controls tumor cell growth and differentiation by interfering with NCAM signaling at cell-cell contacts. Interactions between cells with different PSA and NCAM expression profiles were initiated by enzymatic removal of PSA and by ectopic expression of NCAM or PSA-NCAM. Removal of PSA from the cell surface led to reduced proliferation and activated extracellular signal-regulated kinase (ERK), inducing enhanced survival and neuronal differentiation of neuroblastoma cells. Blocking with an NCAM-specific peptide prevented these effects. Combinatorial transinteraction studies with cells and membranes with different PSA and NCAM phenotypes revealed that heterophilic NCAM binding mimics the cellular responses to PSA removal. In conclusion, our data demonstrate that PSA masks heterophilic NCAM signals, having a direct impact on tumor cell growth. This provides a mechanism for how PSA may promote the genesis and progression of highly aggressive PSA-NCAM-positive tumors

show abstract

Serum polysialylated neural cell adhesion molecule in childhood neuroblastoma

Cited by 46 publications

References 15 publications

Loss of Neural Cell Adhesion Molecule Induces Tumor Metastasis by Up-regulating Lymphangiogenesis

Loss of Neural Cell Adhesion Molecule Induces Tumor Metastasis by Up-regulating Lymphangiogenesis

Expression of the neural cell adhesion molecule and polysialic acid in human neuroblastoma cell lines

Polysialic Acid Directs Tumor Cell Growth by Controlling Heterophilic Neural Cell Adhesion Molecule Interactions

Contact Info

Product

Resources

About