Serum Prolidase Activity and Oxidative Stress in Diabetic Nephropathy and End Stage Renal Disease: A Correlative Study with Glucose and Creatinine

Abstract

Association of oxidative stress and serum prolidase activity (SPA) has been reported in many chronic diseases. The study was aimed at evaluating the correlation of glucose and creatinine to SPA and oxidative stress in patients with diabetic nephropathy (DN) and end stage renal disease (ESRD) concerned with T2DM. 50 healthy volunteers, 50 patients with T2DM, 86 patients with DN, and 43 patients with ESRD were considered as control-1, control-2, case-1, and case-2, respectively. Blood glucose, creatinine, SPA, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured by colorimetric tests. SPA, TOS, and OSI were significantly increased in case-1 and case-2 than control-1 and control-2, while TAS was significantly decreased (P < 0.001). Blood glucose was linearly correlated to SPA, TOS, TAS, and OSI in control-2, case-1 and case-2 (P < 0.001). Serum creatinine was linearly correlated with SPA, TOS, TAS and OSI in control-2 and case-1 (P < 0.001). In case-2, serum creatinine was significantly correlated with SPA only (P < 0.001). Thus, the study concluded that SPA and oxidative stress significantly correlated with blood glucose and creatinine. SPA, TOS, TAS, and OSI can be used as biomarkers for diagnosis of kidney damage.

Involvement of the NF-κB signaling pathway in the renoprotective effects of isorhamnetin in a type 2 diabetic rat model

“…In the present study, the DM group had higher levels of oxidative marker MDA and lower levels of antioxidative marker T-SOD. This was consistent with the studies that reported increased oxidative activity in diabetic subjects (34,35). There is increasing evidence from pre-clinical and clinical studies, which suggests that antioxidant agents have potential ameliorative effects on diabetic complications, including DN (17,36,37).…”

The relationships among the levels of oxidative and antioxidative parameters, FEV1 and prolidase activity in COPD

“…Verma et al 20 reported that prolidase activity, TOS, and OSI were significantly higher in diabetic nephropathy and end-stage renal disease group than in controls, whereas TAS were significantly lower. We also found in this study that prolidase activity and TOS levels were significantly lower and OSI levels were significantly higher in patients with COPD than in controls.…”

Oxidative Stress and Major Depression

“…The increased oxidative stress maintained by different antioxidant system (ascorbic acid, vitamin-E, SOD and so forth) and this antioxidants status altered in different uncontrolled disease condition [29][30][31][32]. Ascorbic acid, an antioxidant, has negative correlation with the major depression is a finding well-established [33].…”