Serum protein electrophoretic pattern in piglets during the early postnatal period

Tóthová, Csilla; Link, R.; Kyzeková, Petronela; Nagy, Oskar

doi:10.1038/s41598-021-96957-6

Cited by 9 publications

(8 citation statements)

References 55 publications

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“…In these animals, antibodies are secreted in the maternal milk and subsequently must be passively received by the fetus from colostrum [65]. The γ-globulin concentrations obtained in this study are comparable to those presented previously in neonatal ruminants [29,31], but comparative studies were not yet performed. The results suggest that electrophoresis may be an important laboratory diagnostic aid to monitor perinatal serum protein electrophoretic pattern and the distribution of serum proteins in newborns of farm animals.…”

Section: Discussionsupporting

confidence: 80%

“…The proportion of albumin was higher than 50% in all the evaluated ruminant species, but the study showed differences in its concentrations between calves, lambs, as well as goat kids. Although the measured values were comparable to those presented previously [29][30][31], comparison studies between different animal species were not yet performed. Variations in the amino acid composition of the albumin, structure and binding sites for ligands were observed among different animal species [32].…”

Section: Discussionsupporting

confidence: 63%

“…Furthermore, electrophoresis in newborn piglets showed a double peak in the albumin zone with two approximately similar lower peaks that were previously not presented in any other species. The second peak on the cathodal side of the albumin zone probably represents the so called postalbumin fraction and its shape and size diminishes with advancing age [31]. According to Lardinois and Page [37], postalbumin could be a fetal-specific protein that disappears with age.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Differences in the Serum Protein Electrophoretic Pattern in Precolostral Serum of Farm Animal Neonates

Tóthová,

Link,

Glembová

et al. 2023

Agriculture

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The objective of this study was to compare the electrophoretic pattern of serum proteins in newborn calves, lambs, goat kids and piglets in precolostral blood samples. Blood samples were collected within 30 min of birth, prior to ingestion of first colostrum, and the concentrations of total proteins and protein fractions were analyzed using electrophoresis on agarose gel. The size and shape of the protein fractions on the electrophoretograms differed among the investigated animal species. Significant differences were found in the total protein values and all the separated protein fractions, as well as albumin to globulin ratio (p < 0.01 and p < 0.001). The mean total protein concentration in piglets was lower compared to ruminants. Albumin concentrations were markedly higher, and the concentrations of α1-globulins were lower, in neonatal ruminants compared to piglets. The values of α2-globulins were higher in lambs and goat kids, and lower in calves and piglets. An opposite tendency was found in the values of β-globulins: a detectable amount of γ-globulins was recorded in all evaluated animal species. Presented results suggest marked species related differences in the shape and size of protein fraction among neonates of farm animal species, and the importance of the evaluation of electrophoretograms with regard to these findings.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Differences in the Serum Protein Electrophoretic Pattern in Precolostral Serum of Farm Animal Neonates

Tóthová,

Link,

Glembová

et al. 2023

Agriculture

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“…[ 33 ] The concentrations of serum proteins in newborn piglets are very low; therefore, adequate biosynthesis and transportation of proteins are essential for their survival of newborn piglets. [ 34 ] Bile secretion is a crucial biochemical process in newborn piglets, and bile acids (the main component of bile) play a central role in the emulsification of luminal fat and participate in the normal fetal development and growth of piglets. [ 35 ] In addition, the metabolites matching these pathways were mainly decreased in Duroc pigs compared to those in the TB and XB pigs.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Analysis Reveals the Potential Relationship Between Sow Colostrum and Neonatal Serum Metabolites in Different Pig Breeds

Cheng

Azad

Ding

et al. 2023

Molecular Nutrition Food Res

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ScopeColostrum composition is an important indicator of newborn piglet survival and growth. However, limited information is available on the association between colostrum metabolites in sows and serum metabolites in neonates. Therefore, the present study aims to determine the metabolites in the colostrum of sows, in the serum of their offspring piglets, and mother‐offspring metabolite correlations in different pig breeds.Methods and resultsColostrum and serum samples are collected from 30 sows and their piglets from three pig breeds (Taoyuan black, TB; Xiangcun black, XB; and Duroc) to analyze the targeted metabolomics. This study identifies 191 metabolites in the colostrum of sows, including fatty acids, amino acids, bile acids, carnitines, carbohydrates, and organic acids, and the concentrations of these metabolites are highest in the TB pigs. Metabolite profiles in sow colostrum and piglet serum differ among Duroc, TB, and XB pigs, and the matching metabolites are mainly enriched in the digestive system and transportation pathways. Furthermore, identification of the associations between metabolites in the colostrum of sows and their neonate sera suggests that metabolite compounds from colostrum are transported to suckling piglets.ConclusionThe present study findings deepen the understanding of the composition of sow colostrum metabolites and the transportation of metabolites from sow colostrum to piglets. The findings also provide insight regarding the development of dietary formulas that resemble the sow colostrum for newborn animals to maintain health and improve the early growth of offspring.

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“…The presence of viremia in the experimental group may be explained by lower levels of neutralizing antibodies against PCV2 due to the use of intradermal vaccination. The level of total protein in blood serum is determined by the combined concentration of its various fractions (Tóthová et al, 2021). Albumin is one of the most common plasma proteins, and its level is typically used as an indicator of liver or kidney function (Carvalho and Machado, 2018;Friedman and Fadem, 2010).…”

Section: Discussionmentioning

confidence: 99%

Biochemical serum characteristics in fattening pigs infected with porcine circovirus type 2

Holda,

Yefimov

2023

TAVM

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Circoviral infection is one of the most widespread disease among pig populations which cause significant economic losses. Plural complications, differences in biology of known strains, wide range of clinical and biochemical changes require the detail study of its pathogenesis. The aim of the study was to identify biochemical alterations in commercial pig herds caused by circoviral infection progress. The study was carried out with use spontaneously infected animals selected from the farming pig herd aged 17-21 weeks. Animals were previously vaccinated with various vaccines and protocols. Viremia was confirmed by RT-PCR method that serves as the main criterium to divide animals for control and experimental groups. The blood samples were collected to detect biochemical parameters with applying an automated biochemical analyzer, high-performance liquid chromatography and inductively coupled plasma emission spectrometry methods. The most higher circovirus concentration observed for 17 weeks of age with consequently reciprocal decrease at 18 weeks and an increase in PCV2 DNA concentration at 19-21 weeks, while it remained significantly consistent during 19-21 weeks. The progress in circoviral infection likely led to a notable increase in total protein content in blood serum at 17th (by 11.6%; P<0.05), 19th (by 12.7%; P<0.05), 20th (by 14.7%), and 21st (by 9.1%) weeks of age. These changes in animal blood from 17 to 20 weeks were attributed to globulins. The globulins content was observed in PCV2 infected pigs at 17, 18, 19, and 20 weeks exceeded control values by 34.4%, 17.7%, 18.4%, and 11.6%, respectively (P<0.05). At 21 weeks, pigs with PCV2 viremia showed a 20.9% higher (P<0.05) level of inorganic phosphorus and nearly twofold higher (P<0.05) alkaline phosphatase activity in blood serum. The selenium concentration in 17-week-old pigs in PCV2 infected group was higher by 17.8% (P<0.05) in respect with control. Contrary, decrease in vitamin E level by 23.2% (P<0.05) and 31.5% (P<0.05) was detected in pig groups for 19 and 21 weeks respectively. The primary biochemical changes in porcine circovirus infection include an increase in total protein content and globulin fractions that accompanied by a decrease in vitamin E level. These findings reflect the impact of PCV-2 infectious progress in swine homeostasis disturbance and can be applied to advance biochemical diagnostics and to construct new therapeutic and preventive measures.

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Serum protein electrophoretic pattern in piglets during the early postnatal period

Cited by 9 publications

References 55 publications

Evaluation of the Differences in the Serum Protein Electrophoretic Pattern in Precolostral Serum of Farm Animal Neonates

Evaluation of the Differences in the Serum Protein Electrophoretic Pattern in Precolostral Serum of Farm Animal Neonates

Metabolomics Analysis Reveals the Potential Relationship Between Sow Colostrum and Neonatal Serum Metabolites in Different Pig Breeds

Biochemical serum characteristics in fattening pigs infected with porcine circovirus type 2

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