Serum proteins and electrophoretic profile in horses undergoing crotalid          venom hyperimmunization

Ramos, Monique Gonçalves Dos Santos Novelino; Campos, Sabrina Destri Emmerick; Strauch, Marcelo A.; Ott, Leila Cardozo; Macieira, Daniel de Barros; Alencar, Nayro Xavier de; Lessa, Daniel Augusto Barroso

doi:10.1294/jes.33.31

Cited by 2 publications

(3 citation statements)

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“…The evaluated serum proteins electrophoretic profile of hyperimmunized horses in this study revealed a significant increase in the concentrations of α1, α2 and γ-globulins in the vaccinated groups. The increase in α1 and α2 globulins particularly in groups administered the combined toxoidtoxin vaccine could implicate as mentioned previously the development of systemic acute phase response to an inflammatory condition induced by vaccination, a finding which is consistent with previous studies carried out on immunized animals (Andersen, 2012;Arfuso et al, 2021 andRamos et al, 2022).…”

Section: Discussionsupporting

confidence: 90%

“…With respect to the serum biochemical studies, the findings of the present investigation showed that serum total protein levels were greater following vaccination compared to the horses in the control group which could be attributed to increased serum globulin levels. Increases in serum proteins have also been observed in horses used for production of polyvalent snake antivenom (Waghmare et al, 2014 andRamos et al, 2022).…”

Section: Discussionmentioning

confidence: 97%

“…In the current investigation the increase in globulin concentrations were greater when a combination of toxoid and toxin was injected compared to the group received only toxoid vaccine.Moreover, the electrophoretic fractions of serum globulin, such as α1 and α2 and gamma globulins, also increased along with the rising trend of serum globulinparticularly in the groups administered the combined toxoid-toxin vaccines (Arfuso et al, 2021 andRamos et al, 2022).…”

Section: Discussionmentioning

confidence: 98%

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Hematobiochemical and Immunological Profiles of Horses Used for Preparation of ‎Antitetanic hyperimmune Serum by Different Types of Adjuvants

Saleh¹,

Allam²,

Elfiky³

et al. 2023

Journal of Current Veterinary Research

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This study aimed to characterize the hematobiochemical and immunological profiles following immunization of equine with tetanus toxoid or/and toxin for obtaining antitetanic hyperimmune serum with special reference to the role of adjuvants in boosting immune response to immunization. Forty-Five apparently healthy horses were divided into 9 groups, one control group and other 8 groups were subjected to 8 vaccination protocols including tetanus toxoid or/ and toxin with or without adjuvants according to the experimental design of this study. Blood samples were collected from all horses and were assayed for the selected hematobiochemical and immunological parameters. Hematologically, there was a significant decreasesin the mean values of RBC, Hb, PCV, MCV and platelet in all vaccinated groups compared to control one. MCH and MCHC values showed a significant increases in immunized horses except in group administered tetanus toxoid combined with CFA whereas their values exhibited a significant decreases. The mean values of TLC, neutrophil, lymphocyte, and monocyte counts exhibited a significant increase in all vaccinated groups with or without addition of adjuvants. The results of serum biochemical parameters showed significant reduction in serum albumin levels and marked elevation in the concentrations of total protein, globulin, total bilirubin, direct bilirubin, indirect bilirubin as well as serum enzymatic activities of AST and LDH. Regarding the immunological responses, all vaccinated groups demonstrated a significant increase in the limits of flocculation, IgG and α1, α2 and γ-globulins compared to control particularly in groups injected with a combination tetanus toxoid and toxin. For most tested parameters the effects were more pronounced when CFA was added to the vaccination protocols. In conclusion, the presented results can consider the Freund adjuvants as apotent enhancers of immune response to tetanus vaccines for production of high titer hyperimmune serum with few side effects and thus high safety margin. However, the repeated use of horses for preparation of antitetanic hyperimmune serum should be Clinical Pathology

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

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