Serum proteome changes and accelerated reduction of fat mass after laparoscopic gastric plication in morbidly obese patients

Savedoroudi, Parisa; Bennike, Tue Bjerg; Kastaniegaard, Kenneth; Talebpour, Mohammad; Ghassempour, Alireza; Stensballe, Allan

doi:10.1016/j.jprot.2019.05.001

Cited by 3 publications

(5 citation statements)

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“…The detailed characteristics of patients were mentioned in Savedoroudi et al. [1] . The six most abundant serum proteins (albumin, IgG, IgA, antitrypsin, transferrin and haptoglobin) were depleted in the serum samples using the Agilent Multiple Affinity Removal column (4.6 × 50 mm) according to the instructions recommended by the manufacturers (Agilent Technologies, CA, USA).…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The six most abundant serum proteins (albumin, IgG, IgA, antitrypsin, transferrin and haptoglobin) were depleted in the serum samples using the Agilent Multiple Affinity Removal column (4.6 × 50 mm) according to the instructions recommended by the manufacturers (Agilent Technologies, CA, USA). Then, the filter-aided sample preparation (FASP) protocol was utilized to prepare samples as described previously [1] , [3] . Written consent was obtained from all participants and the institutional review board and the ethic committee of Shahid Beheshti University approved the study protocol for Human rights (IR.SBU.ICBS.97/1019).…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…This report is associated with the research article aimed at investigating the effect of weight loss due to laparoscopic gastric plication (LGP) as a new bariatric surgical procedure on the human serum proteome [1] . A total of 288 proteins was identified using a shotgun label-free proteomics experiment, of which 224 proteins were quantifiable with at least two unique peptides in 70% of samples or more ( Supplementary Table 1 ).…”

Section: Datamentioning

confidence: 99%

“…Significantly regulated proteins between pre- and post-surgery samples were discussed in detail in Savedoroudi et al. [1] . Gene ontology enrichment analysis for biological process and molecular function of differentially regulated proteins at T1 and T2 are represented in Table 2 and Table 3 , respectively.…”

Section: Datamentioning

confidence: 99%

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Data from quantitative serum proteomic analysis after laparoscopic gastric plication

et al. 2019

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Bariatric surgery is an effective treatment for morbid obesity with a sustained weight loss and improvements in metabolic syndrome. We present a label free quantitative shotgun proteomics approach to analyze the serum proteome of obese people who underwent Laparoscopic Gastric Plication (LGP) as a new bariatric surgery. Pre-surgery serum samples of obese individuals were compared with the serum of the same subjects 1–2 months post-surgery (T1) and 4–5 months post-surgery (T2). The data provide a list of 224 quantifiable proteins with at least two unique peptides that were quantifiable in at least 70% of samples. Gene ontology biological processes and molecular functions of differentially regulated proteins between pre- and post-surgery samples were investigated using WebGestalt online tool. In addition, molecular networks of differentially abundant proteins were determined through Ingenuity Pathway Analysis (IPA) software. This report is related to the research article entitled “Serum proteome changes and accelerated reduction of fat mass after Laparoscopic Gastric Plication in morbidly obese patients” (Savedoroudi et al. [1]). Proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository through the identifier PXD010528.

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Datamentioning

confidence: 99%

Section: Datamentioning

confidence: 99%

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Data from quantitative serum proteomic analysis after laparoscopic gastric plication

et al. 2019

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“…Specifically, serum proteomic analysis serves as an efficient way to identify protein biomarkers to recognize, diagnose, monitor and treat various disorders, such as OB and MetS. 10 11 …”

Section: Introductionmentioning

confidence: 99%

Serum galectin-3BP as a novel marker of obesity and metabolic syndrome in Chinese adolescents

Zhen

Han

et al. 2021

BMJ Open Diab Res Care

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IntroductionChildhood obesity (OB) and metabolic syndrome (MetS) have become a worldwide health problem. Comparative proteomic approaches are widely used in human OB to analyze protein changes in blood plasma. The present study determined the galectin-3 binding protein (galectin-3BP) expression level in different weight categories and assessed the associations between galectin-3BP and OB and MetS.Research design and methodsThe current study included 932 Chinese adolescents 13–18 years of age. The biochemical and anthropometric variables of all the subjects were evaluated using standardized procedures. The differentially expressed proteins (DEPs) were investigated among 60 adolescents (20 normal weight, 20 overweight and 20 obese) using tandem mass tag (TMT) quantitative proteomics. The serum galectin-3BP level was measured using ELISA. The associations between galectin-3BP and OB and MetS were analyzed in 932 adolescents using multiple logistic regression analyses.ResultsA significant DEP, galectin-3BP, can effectively separate the obese from the normal weight group using TMT. Adolescents in tertile 3 of galectin-3BP, when compared with adolescents in the tertile 1, were positively associated with OB (OR=3.32, 95% CI 1.79 to 6.16) and MetS (OR=3.28, 95% CI 1.30 to 8.26). The receiver operating characteristic curve for galectin-3BP in subjects with MetS indicated that the area under the curve was 0.85 (95% CI 0.79 to 0.91).ConclusionsThis study confirmed an association between galectin-3BP and OB in Chinese adolescents, and galectin-3BP was also positively associated with MetS, and thus might be useful for identifying adolescents with MetS.

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Proteomic analysis of overweight/obesity and related abnormal glucose and lipid metabolism caused by phlegm-dampness retention

Sun

Cheng

et al. 2021

Journal of Traditional Chinese Medical Sciences

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Serum proteome changes and accelerated reduction of fat mass after laparoscopic gastric plication in morbidly obese patients

Cited by 3 publications

References 70 publications

Data from quantitative serum proteomic analysis after laparoscopic gastric plication

Data from quantitative serum proteomic analysis after laparoscopic gastric plication

Serum galectin-3BP as a novel marker of obesity and metabolic syndrome in Chinese adolescents

Proteomic analysis of overweight/obesity and related abnormal glucose and lipid metabolism caused by phlegm-dampness retention

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