Serum reference interval values of selected micronutrients, vitamins, and detectable interleukins among healthy adults in south-west Nigeria

Age‐ and sex‐specific reference intervals for superoxide dismutase enzyme and several minerals in a healthy adult cohort

“…In addition, previous cross‐sectional studies have demonstrated that the intake of copper and its serum level is positively associated with each other 50,52 ; therefore, the various dietary patterns can influence the reference value distributions of various geographic areas. Furthermore, the method of copper measurement was equal to our study in Nigeria 48 and Greece 49 studies, though the method of Germany 29 study was electrothermal atomic absorption spectrophotometry.…”

“… The assay methodology used for each study: a The Vitros 5,600 FS analyzer (Ortho Clinical Diagnostics), CLSI C28‐A3 guidelines 38 ; b Olympus AU 400 Chemistry auto analyzer (Olympus Optical, Japan), CLSI C28‐A2 guidelines 31 ; c CLSI C28‐A3 guidelines 4 ; d NCCLS guideline 30 ; e atomic absorption by Perkin‐Elmer instrument 41 ; f automated potentiometric analyzer (the NOVA 8 electrolyte analyzer [NOVA Biomedical, Mississauga, ON, Canada]), NCCLS document C28‐A 42 ; g flame atomic absorption spectrometry 28,48,49 ; h electrothermal atomic absorption spectrophotometry 29 ; i Gaussian curve 27 …”

“…Reference intervals for micro‐minerals of zinc and copper were evaluated. Previous RIs established for zinc are shown in Table 2 for men and women of Tehran (Iran), 28 Nigeria, 48 Greek, 49 and Germany 29 . Method of zinc measurement was flame atomic absorption spectrometry in studies from Tehran, 28 Nigeria, 48 and Greece, 49 though the method applied by the German group was electrothermal atomic absorption spectrophotometry 29 .…”

“…Previous RIs established for zinc are shown in Table 2 for men and women of Tehran (Iran), 28 Nigeria, 48 Greek, 49 and Germany 29 . Method of zinc measurement was flame atomic absorption spectrometry in studies from Tehran, 28 Nigeria, 48 and Greece, 49 though the method applied by the German group was electrothermal atomic absorption spectrophotometry 29 . Sex partitioning was reported as necessary in Greek adults and Iranians, in contrast to our results, while age partitioning was not reported by these studies.…”

“…To compare our results, the recommended RIs for copper are shown for populations from Nigeria, 48 Greece, 49 and Germany 29 in Table 2. Presented RIs in these studies were significantly wider than that established in the current study.…”