Serum α-SMA is a potential noninvasive biomarker of liver fibrosis

Cardoso-Lezama, Irina; Ramos-Tovar, Erika; Arellanes-Robledo, Jaime; Vargas-Pozada, Eduardo E.; Vásquez-Garzón, Verónica Rocío; Villa-Treviño, Saúl; Muriel, Pablo

doi:10.1080/15376516.2023.2244061

Cited by 7 publications

(1 citation statement)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 D), suggesting that m-NG2/ BM MSCs were superior to parental m- BM MSCs in repairing the damaged bile ducts. We used an antibody against muscle actin alpha (ɑ-SMA), a marker for activated hepatic stellate cells (HSCs) [ 36 ] to determine whether this repair could improve liver fibrotic load, and -detected a greater reduction in mice that treated with m- BM MSCs (Fig. 3 Eb) compared to DEN-treated mice (Fig.…”

Section: Resultsmentioning

confidence: 99%

New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis

Hu,

Lai,

Chen

et al. 2024

Stem Cell Res Ther

View full text Add to dashboard Cite

Background Bone marrow-derived mesenchymal stem cell (BMMSC)-based therapy has become a major focus for treating liver fibrosis/cirrhosis. However, although these cell therapies promote the treatment of this disease, the heterogeneity of BMMSCs, which causes insufficient efficacy during clinical trials, has not been addressed. In this study, we describe a novel Percoll–Plate–Wait procedure (PPWP) for the isolation of an active cell subset from BMMSC cultures that was characterized by the expression of neuroglial antigen 2 (NG2/BMMSCs). Methods By using the key method of PPWP and other classical biological techniques we compared NG2/BMMSCs with parental BMMSCs in biological and functional characteristics within a well-defined diethylnitrosamine (DEN)-induced liver fibrosis/cirrhosis injury male C57BL/6 mouse model also in a culture system. Of note, the pathological alterations in the model is quite similar to humans’. Results The NG2/BMMSCs revealed more advantages compared to parentalBMMSCs. They exhibited greater proliferation potential than parental BMMSCs, as indicated by Ki-67 immunofluorescence (IF) staining. Moreover, higher expression of SSEA-3 (a marker specific for embryonic stem cells) was detected in NG2/BMMSCs than in parental BMMSCs, which suggested that the “stemness” of NG2/BMMSCs was greater than that of parental BMMSCs. In vivo studies revealed that an injection of NG2/BMMSCs into mice with ongoing DEN-induced liver fibrotic/cirrhotic injury enhanced repair and functional recovery to a greater extent than in mice treated with parental BMMSCs. These effects were associated with the ability of NG2/BMMSCs to differentiate into bile duct cells (BDCs). In particular, we discovered for the first time that NG2/BMMSCs exhibit unique characteristics that differ from those of parental BMMSCs in terms of producing liver sinusoidal endothelial cells (LSECs) to reconstruct injured blood vessels and sinusoidal structures in the diseased livers, which are important for initiating hepatocyte regeneration. This unique potential may also suggest that NG2/BMMSCs could be an novel off-liver progenitor of LSECs. Ex vivo studies revealed that the NG2/BMMSCs exhibited a similar trend to that of their in vivo in terms of functional differentiation responding to the DEN-diseased injured liver cues. Additionally, the obvious core role of NG2/BMMSCs in supporting the functions of BMMSCs in bile duct repair and BDC-mediated hepatocyte regeneration might also be a novel finding. Conclusions Overall, the PPWP-isolated NG2/BMMSCs could be a novel effective cell subset with increased purity to serve as a new therapeutic tool for enhancing treatment efficacy of BMMSCs and special seed cell source (BDCs, LSECs) also for bioliver engineering.

show abstract

Section: Resultsmentioning

confidence: 99%

New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis

Hu,

Lai,

Chen

et al. 2024

Stem Cell Res Ther

View full text Add to dashboard Cite

show abstract

Nitric oxide donor S‐Nitroso‐N‐acetyl penicillamine for hepatic stellate cells to restore quiescence

Du,

He,

Jia

et al. 2024

Pediatric Discovery

View full text Add to dashboard Cite

Liver fibrosis is a hepatic scar repair response associated with a wide range of liver injuries, which is mediated by an imbalance between extracellular matrix (ECM) synthesis and degradation, leading to massive ECM deposition and disruption of normal liver architecture. Hepatic stellate cells (HSCs) are the main source of ECM during liver fibrosis and are the first identified cell subpopulation associated with liver fibrosis formation. Various current studies on the mechanism and treatment of liver fibrosis require resting‐state HSCs as study subjects. However, spontaneous activation of primary HSCs occurs after 2–3 days of culture after isolation, and it is common that HSCs cell lines gradually differentiate into fibroblasts with culture time. This study provides an induction medium for quiescent HSCs‐containing all‐trans retinoic acid, sodium oleate, and S‐nitroso‐N‐acetyl penicillamine (SNAP)‐and an induction method. The induction method not only maintains the HSCs cell line in a quiescent state but also restores the activated HSCs to a quiescent state. The method has a good induction effect, short induction time, and convenient operation, which is worth being popularized and used in a wide range of laboratories.

show abstract

SIRT1 activation ameliorates rhesus monkey liver fibrosis by inhibiting the TGF-β/smad signaling pathway

Xia,

Luo,

Gao

et al. 2024

Chemico-Biological Interactions

View full text Add to dashboard Cite

Serum α-SMA is a potential noninvasive biomarker of liver fibrosis

Cited by 7 publications

References 40 publications

New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis

New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis

Nitric oxide donor S‐Nitroso‐N‐acetyl penicillamine for hepatic stellate cells to restore quiescence

SIRT1 activation ameliorates rhesus monkey liver fibrosis by inhibiting the TGF-β/smad signaling pathway

Contact Info

Product

Resources

About