Sesamin ameliorates mucosal tissue injury of mesenteric ischemia and reperfusion in an experimental rat model

Abstract

Introduction: Mesenteric ischemia/reperfusion (I/R) injury is a serious clinical condition. There were a lot of experimental studies performed in the treatment of I/R injury. To our knowledge, this is the first experimental study with effects of sesamin on I/R injury model. We aimed to investigate the protective effect of sesamin on mesenteric I/R injury model. Material and methods: A total of 32 male Sprague-Dawley rats were divided into four groups. Control group: superior mesenteric artery (SMA) exposed without clamping. I/R group: SMA was clamped for 60 min and then reperfused for 2 h. Sesamin group (S): 30 mg/kg sesamin were given for 5 days, and SMA exposed without clamping. I/R + S group: 30 mg/kg sesamin were given for 5 days, SMA was clamped for 60 min, and then reperfused for 2 h. Plasma and tissue oxidant parameters were investigated as well as histopathological evaluation. Results: Plasma and tissue total antioxidant status (TAS) levels were significantly higher in I/R + S group compared to the rest (p < 0.005). The plasma TAS levels in I/R group was significantly low. The highest tissue TAS levels were detected in I/R + S group. The high levels of plasma and tissue TOS were found in I/R + S group. Plasma and tissue OSI levels were significantly higher in I/R group. Histopathologic evaluation showed that the mean level of intestinal tissue injury score in I/R group was 2.75 and 1.38 in I/R + S group. Conclusions: Sesamin helps to protect the intestinal tissue at the cellular level by reducing the oxidative stress and inflammation at both the plasma and tissue levels in the experimental I/R model.

Fetuin-A exerts a protective effect against experimentally induced intestinal ischemia/reperfusion by suppressing autophagic cell death

“…36 It was found that I/R injury was accompanied with excess oxygen-free radicals, which caused cell membrane lipid peroxidation, membrane damage, inhibition of mitochondrial activity, and augmented membrane permeability. 37 Furthermore, Chen et al 38 mentioned that the generation of an extreme amount of reactive oxygen species (ROS) in injured cells and tissues during I/R lesion triggered different signaling pathways, promoted inflammatory reactions, and damaged the function of the intestinal mucosal barrier. Our results regarding the state of oxidant/antioxidant imbalance in I/R control group were in line with the results of Mohamed and Almallah 39 and Tassopoulos et al 40 who demonstrated that the MDA level is considered a consistent indicator of an I/R incident, and it increases with the severity of the injury.…”

Health benefits of sesamin on cardiovascular disease and its associated risk factors

“…interferon γ (IFNγ)-inducible protein 10 (IP-10/CXCL10)) in human primary monocytes and THP-1 cells ( Hsieh et al, 2014 ). The anti-inflammatory effects of sesamin have also been observed in different in vivo models ( Cui et al, 2010 , Lin et al, 2014 , Ahmad et al, 2016 , Zhang et al, 2016 , Li et al, 2016 , Fan et al, 2017 , Rousta et al, 2018 , Zhao et al, 2019 , Bai et al, 2019 , Lin et al, 2019 , Sayhan et al, 2019 Ali et al, 2020 ). Consistently, sesamin has been shown to suppress a series of inflammatory markers associated with type-II diabetes and rheumatoid arthritis in humans ( Mohammad Shahi et al, 2017 , Helli et al, 2019 ).…”

Sesamin Protects against and Ameliorates Rat Intestinal Ischemia/Reperfusion Injury with Involvement of Activating Nrf2/HO‐1/NQO1 Signaling Pathway

“…* * P < 0:01 and * * * P < 0:001 vs. the sham group; # P < 0:05, ## P < 0:01, and ### P < 0:001 vs. the I/R and H/R group. 6 Oxidative Medicine and Cellular Longevity reversed by sesamin (Figure 4(e)), suggesting that sesaminreduced intestinal I/R injury was related to the inhibition of oxidative stress. Nrf2 activation is related to suppression of oxidative stress.…”

“…Sesamin (Figure 1), a natural lignin compound, is isolated from sesame seeds, and it possesses health beneficial effects, including anti-inflammatory, anticancer, antihypertensive, antithrombotic, antidiabetic, antiatherogenic, antiobesity, and lipolytic effects [4,5], and ameliorates intestinal, renal, cardiac, brain, and hepatic injury [6][7][8][9][10][11]. However, the key modulatory properties and characteristics of sesamin and related mechanisms in ameliorating intestinal I/R injury remain to be explored.…”