SETDB1 plays an essential role in maintenance of gonocyte survival in pigs

Liu, Tian Tian; Zhang, Pengfei; Li, Tian Jiao; Chen, Xiao Xu; Zhu, Zhen Shuo; Lyu, Ying Hua; Li, Xue Liang; Tian, Xiue; Zeng, Wen Xian

doi:10.1530/rep-17-0107

Cited by 7 publications

(2 citation statements)

References 43 publications

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“…SIRT1 may regulate granulosa cell apoptosis during follicular atresia in porcine ovaries [39] and can reduce ovarian cell viability in rats [40]. SETDB1 plays an essential role in the maintenance of gonocyte survival in pigs, and knockdown of SETDB1 can induce gonocyte apoptosis [41]. PSMD4 effectively inhibits sperm-oocyte binding [42].…”

Section: Discussionmentioning

confidence: 99%

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

Shi

Wang

Liu

et al. 2020

J Animal Sci Biotechnol

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Background: Runs of homozygosity (ROHs) are homozygous segments of the genome where the two haplotypes inherited from the parents are identical. The current availability of genotypes for a very large number of single nucleotide polymorphisms (SNPs) is leading to more accurate characterization of ROHs in the whole genome. Here, we investigated the occurrence and distribution of ROHs in 3,692 Large White pigs and compared estimates of inbreeding coefficients calculated based on ROHs (F ROH), homozygosity (F HOM), genomic relationship matrix (F GRM) and pedigree (F PED). Furthermore, we identified genomic regions with high ROH frequencies and annotated their candidate genes. Results: In total, 176,182 ROHs were identified from 3,569 animals, and all individuals displayed at least one ROH longer than 1 Mb. The ROHs identified were unevenly distributed on the autosomes. The highest and lowest coverages of Sus scrofa chromosomes (SSC) by ROH were on SSC14 and SSC13, respectively. The highest pairwise correlation among the different inbreeding coefficient estimates was 0.95 between F ROH_total and F HOM , while the lowest was − 0.083 between F GRM and F PED. The correlations between F PED and F ROH using four classes of ROH lengths ranged from 0.18 to 0.37 and increased with increasing ROH length, except for ROH > 10 Mb. Twelve ROH islands were located on four chromosomes (SSC1, 4, 6 and 14). These ROH islands harboured genes associated with reproduction, muscular development, fat deposition and adaptation, such as SIRT1, MYPN, SETDB1 and PSMD4. Conclusion: F ROH can be used to accurately assess individual inbreeding levels compared to other inbreeding coefficient estimators. In the absence of pedigree records, F ROH can provide an alternative to inbreeding estimates. Our findings can be used not only to effectively increase the response to selection by appropriately managing the rate of inbreeding and minimizing the negative effects of inbreeding depression but also to help detect genomic regions with an effect on traits under selection.

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Section: Discussionmentioning

confidence: 99%

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

Shi

Wang

Liu

et al. 2020

J Animal Sci Biotechnol

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“…To investigate the function of SETDB1 in porcine SSCs, we knocked down SETDB1 in porcine SSCs by siRNAs [31,37]. The immortalized porcine SSC line was established by stably expressing the simian virus 40 (SV40) large T antigen (Figure 2A) [31].…”

Section: Knockdown (Kd) Of Setdb1 Via Sirnas In Porcine Sscsmentioning

confidence: 99%

SETDB1 Regulates Porcine Spermatogonial Adhesion and Proliferation through Modulating MMP3/10 Transcription

Liu

Guo

et al. 2022

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The transition from gonocytes into spermatogonia takes place during the homing process. A subpopulation of undifferentiated spermatogonia in niche then shifts to spermatogonial stem cells (SSCs), accompanied by the self-renewal ability to maintain life-long fertility in males. Enormous changes in cell morphology, gene expression, and epigenetic features have been reported during spermatogenesis. However, little is known about the difference of these features in SSCs during aging. Here, we examined the dynamics of SET domain bifurcated 1 (SETDB1) expression in porcine testes. SETDB1 was expressed in postnatal undifferentiated spermatogonia, while gradually disappeared after being packed within the basal compartment of seminiferous tubules. In addition, the cell-adhesion ability, proliferative activity, and trimethylation of the histone H3 lysine 9 (H3K9me3) level were significantly altered in SETDB1-deficient porcine SSCs. Moreover, the matrix metalloproteinases 3/10 (MMP3/10) was upregulated at both mRNA and protein levels. These results illustrate the significance of SETDB1 in modulating early male germ cell development.

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Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway

Liu

Chen

et al. 2017

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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SETDB1 plays an essential role in maintenance of gonocyte survival in pigs

Cited by 7 publications

References 43 publications

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

SETDB1 Regulates Porcine Spermatogonial Adhesion and Proliferation through Modulating MMP3/10 Transcription

Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway

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