Setting a new standard: Updating the vaginal distention translational model for stress urinary incontinence

Boncher, Nicholas; Vricella, Gino J.; Kavran, Michael; Xiao, Nan; Hijaz, Adonis

doi:10.1002/nau.21168

Cited by 12 publications

(8 citation statements)

References 16 publications

(21 reference statements)

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“…We also tested the levels of creatine phosphokinase and lactate dehydrogenase, caspase‐3 gene and acetylcholine receptor expression in the pelvic floor muscle after childbirth. All these tests contribute to the pathogenesis of postpartum SUI 8 …”

Section: Introductionmentioning

confidence: 99%

Modified vaginal dilation rat model for postpartum stress urinary incontinence

Huang

Cheng

Ding

et al. 2012

J of Obstet and Gynaecol

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Section: Introductionmentioning

confidence: 99%

Modified vaginal dilation rat model for postpartum stress urinary incontinence

Huang

Cheng

Ding

et al. 2012

J of Obstet and Gynaecol

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“…to 16Fr.) as they were lubricated with Surgilube (Fougera, Melville, NY) 4-6,29 . Then, a modified 6-Fr.…”

Section: Methodsmentioning

confidence: 99%

“…To explore the role of vaginal delivery in the development of SUI, rodent models of simulated maternal childbirth injury have been developed, utilizing vaginal distention (VD), 4-7 pudendal nerve crush, bilateral pudendal nerve transection (PNT) and pubo-urethral ligament transection (PUL). 5,8,9 VD mimics the non-specific trauma associated with the second stage of labor in women whereas pudendal nerve crush and PNT aim to simulate the nerve injury observed with vaginal delivery directly.…”

Section: Introductionmentioning

confidence: 99%

Stem Cell Homing Factor, CCL7, Expression in Mouse Models of Stress Urinary Incontinence

Hijaz

Grimberg

Tao

et al. 2013

Female Pelvic Medicine &Amp; Reconstructive Surgery

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Objectives Animal models of vaginal distention (VD) have demonstrated increased expression of chemokine (C-C motif) ligand 7 (CCL7). In this study, we investigated the expression of CCL7 in mice models of simulated birth trauma-induced urinary incontinence utilizing VD and pudendal nerve transection (PNT). Methods Forty-nine mice were divided into 6 groups: VD, sham VD, PNT, sham PNT anesthesia, and age-matched controls. The urethra, vagina, and rectum were harvested for the expression of CCL7 immediately or 24 hours after assigned procedure. Venous sampling for quantification of serum CCL7 was also performed. An ANOVA model was used to compare the relative expression of CCL7 in each group. Results Urethral CCL7 expression in the VD group was significantly higher than control group after 24 hours (p<0.01). There was no difference in the urethral CCL7 expression in PNT, sham PNT, sham VD or anesthesia groups compared with controls. No statistically significant difference was noted in the vaginal and rectal expression of CCL7 between any of the groups except for sham PNT. Statistically significant differences were noted in the serum CCL7 expression in VD, PNT and sham PNT (p<0.01 in all) groups after 24 hours compared with the control group. Conclusions This study demonstrates over-expression of urethral CCL7 after VD but not PNT. This suggests that nerve injury does not contribute to the CCL7 over-expression. The over-expression of CCL7 in the serum of mice after VD suggests a translational potential where CCL7 measurement could be used as a surrogate for injury after delivery.

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“…Presently, human studies are based on clinical findings of a high incidence of UI 2 years after GDM. The role of GDM in the pathophysiological development of UI remains unclarified [15]. Investigations linking both entities with the synergistic co-activity of the PFM and RAM are insufficient [16].…”

Section: Introductionmentioning

confidence: 99%

Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus

Rudge¹,

Abbade²,

Hallur³

et al. 2020

BMC Pregnancy Childbirth

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Background: Pelvic floor muscles (PFM) and rectus abdominis muscles (RAM) of pregnant diabetic rats exhibit atrophy, co-localization of fast and slow fibers and an increased collagen type I/III ratio. However, the role of similar PFM or RAM hyperglycemic-related myopathy in women with gestational diabetes mellitus (GDM) remains poorly investigated. This study aims to assess the frequency of pelvic floor muscle disorders and pregnancy-specific urinary incontinence (PS-UI) 12 months after the Cesarean (C) section in women with GDM. Specifically, differences in PFM/ RAM hyperglycemic myopathy will be evaluated. Methods: The Diamater is an ongoing cohort study of four groups of 59 pregnant women each from the Perinatal Diabetes Research Centre (PDRC), Botucatu Medical School (FMB)-UNESP (São Paulo State University), Brazil. Diagnosis of GDM and PS-UI will be made at 24-26 weeks, with a follow-up at 34-38 weeks of gestation. Inclusion in the study will occur at the time of C-section, and patients will be followed at 24-48 h, 6 weeks and 6 and 12 months postpartum. Study groups will be classified as (1) GDM plus PS-UI; (2) GDM without PS-UI; (3) Non-GDM plus PS-UI; and (4) Non-GDM without PS-UI. We will analyze relationships between GDM, PS-UI and hyperglycemic myopathy at 12 months after C-section. The mediator variables to be evaluated include digital palpation, vaginal squeeze pressure, 3D pelvic floor ultrasound, and 3D RAM ultrasound. RAM samples obtained during C-section will be analyzed for ex-vivo contractility, morphological, molecular and OMICS profiles to further characterize the hyperglycemic myopathy. Additional variables to be evaluated include maternal age, socioeconomic status, educational level, ethnicity, body mass index, weight gain during pregnancy, quality of glycemic control and insulin therapy. Discussion: To our knowledge, this will be the first study to provide data on the prevalence of PS-UI and RAM and PFM physical and biomolecular muscle profiles after C-section in mothers with GDM. The longitudinal design allows for the assessment of cause-effect relationships between GDM, PS-UI, and PFMs and RAMs myopathy. The findings may reveal previously undetermined consequences of GDM.

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Setting a new standard: Updating the vaginal distention translational model for stress urinary incontinence

Abstract: VD injury based on TP is feasible and reproducible. Understanding catheter pressure dynamics is valuable for investigating alternative rat phenotypes.

Cited by 12 publications

References 16 publications

Modified vaginal dilation rat model for postpartum stress urinary incontinence

Modified vaginal dilation rat model for postpartum stress urinary incontinence

Stem Cell Homing Factor, CCL7, Expression in Mouse Models of Stress Urinary Incontinence

Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus

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