Annotation. The National Academy of Medical Sciences of Ukraine over its thirty-year history has made, without exaggeration, a tremendous contribution to the field of theoretical, clinical and preventive medicine both in Ukraine and abroad, which fundamentally changed the quality of human life in all spheres. The NAMS of Ukraine today has a powerful creative potential, a high reputation in Ukraine and abroad. The Laboratory of Hygiene of Planning and Development of Settlements of the SI “IPH NAMSU” has been developing preventive medicine and standing guard over public health for 90 years and strengthening the prestige of the NAMS of Ukraine in this field. This is the only laboratory of this profile both in the system of the National Academy of Medical Sciences of Ukraine and in the healthcare system of Ukraine. The purpose of the study is to reveal the formation and development of scientific research of the Laboratory of Hygiene Planning and Development of Settlements and to highlight scientific achievements to mark the 90th anniversary of the laboratory of the same name and the 30th anniversary of the National Academy of Medical Sciences of Ukraine. Research methods: the analytical method is used in the work (studying the results of the laboratory of hygiene planning and development of settlements); the method of hygienic substantiation of the reconstruction of settlements. Results: the article highlights the activities of the hygiene laboratory of planning and construction of settlements of the State University “IHZ NAMNU”, which is aimed at solving the most urgent sanitary and hygienic problems of urban and rural construction, regional planning and development of territories and industrial development, environmental protection of settlements, as well as issues ensuring favorable living conditions for the population to preserve and strengthen their health. So, the activities of the Laboratory of Hygiene of planning and construction of settlements of the State University “IGZ NAMNU” on its glorious 90th anniversary, its scientific achievements, which were reflected in sanitary and urban planning legislation and implemented in the public health system of Ukraine, bodies of the State Production and Consumer Service, divisions of the Ministry of Regions, have of particular importance for the further stable development of domestic production of independent Ukraine and the preservation of public health, and strengthens the prestige of the NAMS of Ukraine on its 30th anniversary from the day of its establishment.