Several geranylgeranyl diphosphate synthase isoforms supply metabolic substrates for carotenoid biosynthesis in tomato

Barja, M. Victoria; Ezquerro, Miguel; Diretto, Gianfranco; Florez‐Sarasa, Igor; Feixes, Elisenda; Fiore, Alessia; Karlova, Rumyana; Fernie, Alisdair R.; Beekwilder, Jules; Rodríguez‐Concepción, Manuel

doi:10.1101/2020.07.08.194076

Cited by 3 publications

(1 citation statement)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of PSY1 is boosted during the ripening process, thereby promoting the generation of carotenoids that contribute to fruit pigmentation (Kachanovsky et al, 2012). Previous studies have found that unlike other plants such as Arabidopsis, rice or pepper, which rely on a single GGPPS isoform for the production of the required plastid-like isoprenoids, the production of a majority of the GGPP in the chloroplasts of tomato is dependent on two GGPPS homologues (Zhou et al, 2017;Wang et al, 2018;Barja et al, 2021). Their experiments also confirmed that PSY1 expression was significantly increased in fruit ripening.…”

Section: Slnap2 Influences Chlorophyll and Carotenoid Metabolism In T...mentioning

confidence: 99%

SlNAP2promoted fruit ripening by directly binding theACS2promoter and interacting withEIL3

Wu,

Fang,

Huang

et al. 2024

Preprint

View full text Add to dashboard Cite

The ripening process of tomato fruit is affected by a variety of environmental factors and genetic regulators. NAC transcription factors (TFs) function in a multitude of biological processes, while the current knowledge on the participation of NAC TFs in the regulatory network of fruit ripening is relatively limited. In this study, we isolated a NAC TFs, NAP2, which acts as a positive transcription activator in tomato fruit ripening. We also observed a notable delay in the ripening process ofSlNAP2silenced and knockout mutant fruit. In particular, ethylene production was obviously inhibited inSlNAP2mutant fruit. Y1H and DLR assays showed that SlNAP2 directly binds to the promoter ofSlACS2and activates its transcriptional activity. Furthermore, SlNAP2 and SlEIL3 physically interaction was demonstrated by yeast two-hybrid (Y2H), luciferase complementation (LUC), bimolecular fluorescence complementation (BiFC) and coimmunoprecipitation analysis (CoIP) assays. Meanwhile, the pigment content, firmness and the transcript levels of genes associated with carotenoid and chlorophyll metabolism and cell wall metabolism were also potentially affected by theSlNAP2deletion; however, it has remained unclear whether these genes are also directly regulated bySlNAP2. Therefore, our findings indicate that SlNAP2 directly binds toSlACS2promoter to activate its expression and promote ethylene generation, which in turn interacts with EIL3 to enhance the function of ethylene in tomato fruit ripening. Collectively, our data contribute to understanding the interaction of NAC TFs and ethylene in tomato fruit ripening, thereby enhancing our knowledge of the ripening regulatory network that governs tomato fruit maturation.

show abstract

Section: Slnap2 Influences Chlorophyll and Carotenoid Metabolism In T...mentioning

confidence: 99%

SlNAP2promoted fruit ripening by directly binding theACS2promoter and interacting withEIL3

Wu,

Fang,

Huang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Seeing the unseen: A trifoliate (MYB117) mutant allele fortifies folate and carotenoids in tomato fruits

Tyagi

Sunkum

Rai

et al. 2021

Preprint

View full text Add to dashboard Cite

Micronutrient deficiency also termed hidden hunger affects a large segment of the human population, particularly in developing and underdeveloped nations. Tomato the second most consumed vegetable crop in the world after potato can serve as a sustainable source to alleviate micronutrient deficiency. In tomato, the mutations in the R2R3-MYB117 transcription factor elicit trifoliate leaves and initiate axillary meristems; however, its effect on fruit metabolome remains unexplored. The fruits of a new trifoliate (tf) allele (tf-5) were firmer, had higher Brix, folate, and carotenoids. The transcriptome, proteome, and metabolome profiling of tf-5 reflected a broad-spectrum change in homeostasis. The tf-5 allele enhanced the fruit firmness by suppressing cell wall softening-related proteins. The tf-5 fruit displayed a substantial increase in aminome, particularly γ-aminobutyric acid, with a parallel reduction in aminoacyl t-RNA synthases. The increased lipoxygenases proteins and transcripts seemingly elevated jasmonic acid. In addition, increased abscisic acid hydrolases transcripts coupled with reduced precursor supply lowered abscisic acid. The upregulation of carotenoids was mediated by modulation of methylerythreitol and plastoquinone pathways along with an increase in carotenoids isomerization proteins. The upregulation of folate in tf-5 was connoted by the increase in precursor p-aminobenzoic acid and transcripts of several folate biosynthesis pathway genes. The reduction in pterin-6-carboxylate and γ-glutamyl hydrolase activity indicated that the diminished folate degradation also enriched folate levels. Our study delineates that introgression of the tf-5 can be used for the γ-aminobutyric acid, carotenoids, and folate fortification of tomato.

show abstract

Transcriptome-Based WGCNA Analysis Reveals Regulated Metabolite Fluxes between Floral Color and Scent in Narcissus tazetta Flower

Yang

Ren

Zhang

et al. 2021

IJMS

View full text Add to dashboard Cite

A link between the scent and color of Narcissus tazetta flowers can be anticipated due to their biochemical origin, as well as their similar biological role. Despite the obvious aesthetic and ecological significance of these colorful and fragrant components of the flowers and the molecular profiles of their pigments, fragrant formation has addressed in some cases. However, the regulatory mechanism of the correlation of fragrant components and color patterns is less clear. We simultaneously used one way to address how floral color and fragrant formation in different tissues are generated during the development of an individual plant by transcriptome-based weighted gene co-expression network analysis (WGCNA). A spatiotemporal pattern variation of flavonols/carotenoids/chlorophyll pigmentation and benzenoid/phenylpropanoid/ monoterpene fragrant components between the tepal and corona in the flower tissues of Narcissus tazetta, was exhibited. Several candidate transcription factors: MYB12, MYB1, AP2-ERF, bZIP, NAC, MYB, C2C2, C2H2 and GRAS are shown to be associated with metabolite flux, the phenylpropanoid pathway to the production of flavonols/anthocyanin, as well as related to one branch of the phenylpropanoid pathway to the benzenoid/phenylpropanoid component in the tepal and the metabolite flux between the monoterpene and carotenoids biosynthesis pathway in coronas. It indicates that potential competition exists between floral pigment and floral fragrance during Narcissus tazetta individual plant development and evolutionary development.

show abstract

Several geranylgeranyl diphosphate synthase isoforms supply metabolic substrates for carotenoid biosynthesis in tomato

Cited by 3 publications

References 84 publications

SlNAP2promoted fruit ripening by directly binding theACS2promoter and interacting withEIL3

SlNAP2promoted fruit ripening by directly binding theACS2promoter and interacting withEIL3

Seeing the unseen: A trifoliate (MYB117) mutant allele fortifies folate and carotenoids in tomato fruits

Transcriptome-Based WGCNA Analysis Reveals Regulated Metabolite Fluxes between Floral Color and Scent in Narcissus tazetta Flower

Contact Info

Product

Resources

About