Severe Diabetes Induction as a Generational Model for Growth Restriction of Rat

Cruz, Larissa Lopes da; Barco, Vinícius Soares; Paula, Verônyca Gonçalves; Gallego, Franciane Quintanilha; Souza, Maysa Rocha; Corrente, José Eduardo; Zambrano, Elena; Volpato, Gustavo Tadeu; Damasceno, Débora Cristina

doi:10.1007/s43032-023-01198-9

Cited by 3 publications

(1 citation statement)

References 51 publications

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“…The puppies from the group of diabetic mothers had higher mortality rates. Many studies report how unfavorable the maternal environment becomes when mothers are in hyperglycemia, which can alter placental maturation, making it insufficient in its function of fetal nutrition, causing harm to the development of the offspring (Ejdesjö, Wentzel & Eriksson, 2012;Da Cruz et al, 2023). One of the factors involved in these complications caused by GDM is the oxidative damage caused by excess glucose in maternal-fetal blood (Jakuš et al, 2014).…”

Section: Standardization Of An Efficient Experimental Gestational Dia...mentioning

confidence: 99%

Standardization of an Efficient Experimental Gestational Diabetes Protocol

Mascarenhas,

Ribeiro,

Silva

et al. 2024

Rev. Foco

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Diabetes mellitus (DM) is characterized by a dysfunction in insulin secretion and/or action which causes hyperglycemia. When the disease occurs during pregnancy, it is called gestational diabetes mellitus (GDM), and hyperglycemia affects the fetus's development. The present study aimed to standardize an efficient model of GDM induction in Wistar rats using streptozotocin (STZ). Therefore, the animals are placed for mating overnight, and the next day the presence of sperm is verified in the vaginal wash, indicating the first day of gestation (G1), and after five days (G5), the pregnant rats were induced to diabetes, intraperitoneally, using STZ, at a dose of 50 mg/kg. The animals were weighed, and their blood glucose levels were measured (pre-mating, G7, and G17), being considered diabetics when blood glucose ≥ 200 mg/dL. After birth, the puppies were evaluated for the number of individuals, gender, and stillbirths. The blood of mothers was collected to assess oxidative damage caused by diabetes. The results showed efficacy in the use of the alternative dye gentian violet for sperm and cell identification. Weight gain, hyperglycemia, and the presence of oxidative damage showed an efficient GDM induction for 55.6% of total animals, considered a successful protocol superior to those commonly observed by those researchers in the GDM study area. Also, we observed a higher number of stillbirths in the GDM group, confirming data from the literature that characterize the hyperglycemic environment as hostile to the development of the fetus.

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Section: Standardization Of An Efficient Experimental Gestational Dia...mentioning

confidence: 99%

Standardization of an Efficient Experimental Gestational Diabetes Protocol

Mascarenhas,

Ribeiro,

Silva

et al. 2024

Rev. Foco

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Toxicological effects of the Curatella americana extract in embryo development of female pups from diabetic rats

Cruz,

Barco,

Paula

et al. 2023

Reproductive Biology

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Phytochemical characterization and antidiabetic analysis of Bauhinia holophylla extract on the maternal-fetal outcomes of rats

SAVAZZI,

CRUZ,

MORAES-SOUZA

et al. 2024

An. Acad. Bras. Ciênc.

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This study aims to evaluate the phytochemical properties of Bauhinia holophylla (Bong.) Steud leaf extract, and their impact on maternal reproductive and fetal development in diabetic rats. For this, adult female Wistar rats (100 days of life) received streptozotocin (40 mg/Kg, intraperitoneal) for induction of diabetes, were mated and distributed into four groups: Nondiabetic; Nondiabetic given B. holophylla; Diabetic; and Diabetic given B. holophylla. The plant extract was given by gavage at increasing doses: 200, 400, and 800 mg/Kg. At day 21 of pregnancy, liver and blood samples were obtained for oxidative parameters and biochemical analysis, respectively.The uterus was removed for maternal-fetal outcomes. Phytochemical analysis showed a high content of phenolic components and biogenic amines. B. holophylla extract did not alter the glycemic levels but improved the lipid profile in diabetic animals. Besides that, the number of live fetuses and maternal weight gain were decreased in Diabetic group, and were not observed in animals treated. The group Diabetic treated presented a higher percentage of fetuses classified as adequate for gestational age compared to the Diabetic group. However, the treatment with plant extract caused embryo losses, fetal growth restriction, and teratogenicity in nondiabetic rats. Thus, the indiscriminate consumption requires carefulness.

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Severe Diabetes Induction as a Generational Model for Growth Restriction of Rat

Cited by 3 publications

References 51 publications

Standardization of an Efficient Experimental Gestational Diabetes Protocol

Standardization of an Efficient Experimental Gestational Diabetes Protocol

Toxicological effects of the Curatella americana extract in embryo development of female pups from diabetic rats

Phytochemical characterization and antidiabetic analysis of Bauhinia holophylla extract on the maternal-fetal outcomes of rats

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