Severe Pre-Eclampsia Complicated by Hellp Syndrome in the Hoffbauer and Syncytiotrophoblast Cells (Ultrastructural and Immunohistochemical Study)

Abstract

The aim of this study was to evaluate the immunohistochemistry and ultrastructural of syncytiotrophoblast and Hoffbauer cells in placental villi of HELLP syndrome patients. Placental samples with twenty control (normotensive) and twenty HELLP syndrome were fixed in 10% neutral formalin solution for paraffin-embedding wax protocol. Placental samples were fixed in 2.5% buffered glutaraldehyde and then, in 1% osmium tetra oxide for routine electron microscopic procedure. When the HELLP group fetal placental sections were examined, intracytoplasmic edema in syncytiotrophoblast, degenerative vacuoles and degenerative findings on cell surface membranes were observed. Moreover, villous edema was remarkable. The number of CD68 positive Hoffbauer cells per villus control group sections was 0.23 ± 0.02 and the number of CD68 positive cells per villus in HELLP group placenta sections was 0.83 ± 0.12 . The increase in the number of Hoffbauer cells per villus in the HELLP group was statistically significant (p

Role of SOX9 and Hif-1α expression in placentas of patients with HELLP

“…In the histopathology of placenta, intervillous thrombosis, abruption and infarction were more common in women with HELLP syndrome. Nergiz et al 23 found that Hoffbauer cells were increased in placenta of HELLP syndrome. In their ultrastructural analysis, degenerative structure in cell surface membrane, intracytoplasmic edema, and degenerative vacuoles in syncytiotrophoblast were recorded.…”

Hypertension, preeclampsia, and HELLP syndrome in pregnancy

“…HELLP syndrome and preeclampsia can have a familial tendency. In this regard, Nergiz et al [5] reported a significant increase in placental Hoffbauer cells, syncytiotrophoblast cell counts as well as several ultrastructural changes that were observed in the HELLP group compared with the control group. HELLP occurs in less than 1% of pregnancies and, in most cases, coexists with preeclampsia.…”