Sex differences in the single prolonged stress model

Keller, Samantha M.; Schreiber, William B.; Staib, Jennifer M.; Knox, Dayan

doi:10.1016/j.bbr.2015.02.034

Cited by 69 publications

(65 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, some studies have pointed to different results when males and females are evaluated in studies involving obesity [2] and stress [4,32,42,52]. Especially on stress, various stressor agents and protocols have been used.…”

Section: Introductionmentioning

confidence: 97%

Predictive behaviors for anxiety and depression in female Wistar rats subjected to cafeteria diet and stress

Estrela

Silva

Guimarães

et al. 2015

Physiology & Behavior

View full text Add to dashboard Cite

Obesity and chronic stress have been considered important public health problems that affect millions of people worldwide. Our aim was to analyze the effect of obesity associated with chronic stress on neurobehavioral parameters in female rats, considering that the association of these syndromes can enhance the negative effects on homeostasis. The animals were distributed into standard diet (Std), standard diet+stress (Std+stress), cafeteria diet (Cafe), and cafeteria diet+stress (Cafe+stress) groups. The animals of groups Std and Std+stress were fed with rodent standard feed. Groups Cafe and Cafe+stress, additionally to the standard feed, were offered palatable and calorie-rich processed food and cola-type soft drink ad libitum. From the eighth experimental week, groups Std+stress and Cafe+stress were subjected to restraint chronic stress model (50 days). After the stress protocol, predictive anxiety (open-field and elevated plus-maze tests) and depression (forced swim) were applied. The cafeteria diet was effective in inducing obesity. The ratio locomotion in the central quadrants/total locomotion evaluated during the open field test was not indicative of anxiogenic or anxiolytic effect in the animal's behavior. However, the elevated plus maze test showed that obese and stressed animals were prone to higher anxiety levels. In addition, the obese and stressed animals display less climbing behavior than all the other groups, which can be considered an indicator of depression-like behavior. Nevertheless, it is suggested that the mechanisms involved in effects of obesity associated with chronic stress be better investigated in female rats, considering the organic complexity related to these modern illnesses.

show abstract

Section: Introductionmentioning

confidence: 97%

Predictive behaviors for anxiety and depression in female Wistar rats subjected to cafeteria diet and stress

Estrela

Silva

Guimarães

et al. 2015

Physiology & Behavior

View full text Add to dashboard Cite

show abstract

“…First, only male animals were used in order to simplify the interpretation of this study's results and because previous research on SPS and cocaine sensitization on which this study was based has largely been conducted using male animals. However, studies demonstrating sex differences in response to SPS (Keller, Schreiber, Staib, & Knox, 2015) underscore the importance of including female subjects in future studies. In this study, rats were sacrificed at a single time point (Henry, Hu, & White, 1998), and induced plasticity in glutamatergic synapses onto DA cells in the VTA and its targets (van Huijstee & Mansvelder, 2015).…”

Section: Summary Of Effectsmentioning

confidence: 99%

Divergent effects of repeated cocaine and novel environment exposure on locus coeruleus c‐fos expression and brain catecholamine concentrations in rats

et al. 2019

View full text Add to dashboard Cite

Introduction Chronic administration of cocaine causes a disinhibited, hyperexploratory response to novel environments. As the norepinephrine (NE) system regulates exploration and is dysregulated following cocaine exposure, we hypothesized that this cocaine‐mediated hyperexploratory response is associated with increased locus coeruleus (LC) reactivity. Methods To test this hypothesis, we used dual fluorescent in situ hybridization immunofluorescence to analyze novelty‐induced c‐fos and tyrosine hydroxylase expression in the LC and high‐pressure liquid chromatography to measure dopamine (DA) and NE concentrations in key catecholamine projection regions following exposure to cocaine. Results Repeated cocaine exposure followed by a 14‐day drug‐free period increased exploration of novel environments, replicating previous findings. Novelty exposure increased LC c‐fos expression, increased anterior cingulate NE, and decreased ventral tegmental area DA. Cocaine exposure decreased amygdala (AMY) DA, but had no effect on LC c‐fos expression or NE in any tested brain region. No interactions between cocaine and novelty were found. Open arm exploration was positively correlated with LC c‐fos expression and NE concentrations in both the anterior cingulate and nucleus accumbens, and negatively correlated with AMY DA concentration. Conclusions Our findings confirm that exposure to novel environments increases LC activity and NE in the anterior cingulate cortex, that long‐term exposure to cocaine dysregulates AMY DA, and that disinhibited exploration in novel environments correlates with NE and DA in regions that modulate risk‐taking and avoidance behavior. Further studies investigating the effects of cocaine on brain catecholamine systems are important in understanding the long‐lasting effects of cocaine on brain function.

show abstract

“…To test this hypothesis, we microinfused SR57227, a selective 5-HT 3 receptor agonist (Bachy et al 1993;Edwards et al 1996), into dorsal hippocampus (DH) immediately following a single prolonged stress (SPS) treatment in rats, which is a nonhuman animal model of PTSD (Yamamoto et al 2009;Keller et al 2015;Goswami et al 2013). Three weeks later, we evaluated the rat general anxiety behaviors and the extinction of contextual fear memory.…”

Section: Introductionmentioning

confidence: 99%

Contribution of Hippocampal 5-HT3 Receptors in Hippocampal Autophagy and Extinction of Conditioned Fear Responses after a Single Prolonged Stress Exposure in Rats

Yang

Cui

et al. 2016

Cell Mol Neurobiol

View full text Add to dashboard Cite

One of the hypotheses about the pathogenesis of posttraumatic stress disorder (PTSD) is the dysfunction of serotonin (5-HT) neurotransmission. While certain 5-HT receptor subtypes are likely critical for the symptoms of PTSD, few studies have examined the role of 5-HT receptor in the development of PTSD, even though 5-HT receptor is critical for contextual fear extinction and anxiety-like behavior. Therefore, we hypothesized that stimulation of 5-HT receptor in the dorsal hippocampus (DH) could prevent hippocampal autophagy and the development of PTSD-like behavior in animals. To this end, we infused SR57227, selective 5-HT agonist, into the DH after a single prolonged stress (SPS) treatment in rats. Three weeks later, we evaluated the effects of this pharmacological treatment on anxiety-related behaviors and extinction of contextual fear memory. We also accessed hippocampal autophagy and the expression of 5-HT subunit, Beclin-1, LC3-I, and LC3-II in the DH. We found that SPS treatment did not alter anxiety-related behaviors but prolonged the extinction of contextual fear memory, and such a behavioral phenomenon was correlated with increased hippocampal autophagy, decreased 5-HT expression, and increased expression of Beclin-1 and LC3-II/LC3-I ratio in the DH. Furthermore, intraDH infusions of SR57227 dose-dependently promoted the extinction of contextual fear memory, prevented hippocampal autophagy, and decreased expression of Beclin-1 and LC3-II/LC3-I ratio in the DH. These results indicated that 5-HT receptor in the hippocampus may play a critical role in the pathogenesis of hippocampal autophagy, and is likely involved in the pathophysiology of PTSD.

show abstract

Sex differences in the single prolonged stress model

Cited by 69 publications

References 18 publications

Predictive behaviors for anxiety and depression in female Wistar rats subjected to cafeteria diet and stress

Predictive behaviors for anxiety and depression in female Wistar rats subjected to cafeteria diet and stress

Divergent effects of repeated cocaine and novel environment exposure on locus coeruleus c‐fos expression and brain catecholamine concentrations in rats

Contribution of Hippocampal 5-HT3 Receptors in Hippocampal Autophagy and Extinction of Conditioned Fear Responses after a Single Prolonged Stress Exposure in Rats

Contact Info

Product

Resources

About