Sex-specific DoublesexM expression in subsets of Drosophilasomatic gonad cells

Hempel, Leonie U.; Oliver, Brian

doi:10.1186/1471-213x-7-113

Cited by 69 publications

(72 citation statements)

References 50 publications

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“…In wt testes, Tj is produced in early SCCs and in elongated SCCs wrapping 2-, 4-, 8-cell cysts ( Figure 1B), whereas Eya is present in SCCs enclosing larger cysts ( Figure 2A) [28]. In dlg testes of 3-day-old larvae ( Figure 1C), we found that the number of positively stained Tj cells was comparable to that in wt ( Figure 1B).…”

Section: Reduced Number Of Gscs and Scs In Dlg Larval Testesmentioning

confidence: 56%

discs large regulates somatic cyst cell survival and expansion in Drosophila testis

Papagiannouli

Mechler

2009

Cell Res

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Gonad development requires a coordinated soma-germline interaction that ensures renewal and differentiation of germline and somatic stem cells to ultimately produce mature gametes. The Drosophila tumour suppressor gene discs large (dlg) encodes a septate junction protein functioning during epithelial polarization, asymmetric neuroblast division, and formation of neuromuscular junctions. Here, we report the role of dlg in testis development and its critical function in somatic cyst cells (SCCs). In these cells dlg is primarily required for their survival and expansion, and contributes to spermatocyte cyst differentiation. Cell death primarily occurred in SCCs at the end of spermatogonial amplification at a time when Dlg becomes restricted in wild-type (wt) testes to the distal somatic cells capping the growing spermatocyte cysts. RNAi depletion of dlg transcripts in early SCCs fully prevented testis development, whereas depletion in late SCCs resulted in a breakdown of spermatocyte cyst structure and germ cell individualization. Specific dlg expression in SCCs resulted in developmental rescue of dlg mutant testes, whereas its expression in germ cells exerted no such effect. dlg overexpression in wt testes led to spermatocyte cyst expansion at the expense of spermatogonial cysts. Our data demonstrate that dlg is essentially required in SCCs for their survival, expansion, and differentiation, and for the encapsulation of the germline cells.

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Section: Reduced Number Of Gscs and Scs In Dlg Larval Testesmentioning

confidence: 56%

discs large regulates somatic cyst cell survival and expansion in Drosophila testis

Papagiannouli

Mechler

2009

Cell Res

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“…7 A,A') whereas Eya is present in late SCCs enclosing larger cysts (Fig. 7D) (Hempel and Oliver, 2007). In scrib and lgl testes the number of positively stained TJ-cells was comparable to that of the wt testes (Fig.…”

Section: D' C'mentioning

confidence: 72%

The internal structure of embryonic gonads and testis development in Drosophila melanogaster requires scrib, lgl and dlg activity in the soma

Papagiannouli¹

2013

Int. J. Dev. Biol.

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Interest in the mechanism leading to the formation of the germline and its differentiation during Drosophila development, initiated even as soon as the first ever cloned tumour suppressor gene in Drosophila, the lethal (2) giant larvae (lgl), had been identified. Further work has shown that the lgl, as well as discs large-1 (dlg) and scribble (scrib) tumor suppressor genes code for scaffolding proteins associated with either the cytoskeletal matrix or the septate junctions that act in common pathways in various tissues. This study analysed the role of Dlg, Scrib and Lgl in the embryonic gonads and testis of Drosophila melanogaster. Loss of scrib, dlg and lgl had no effect on gonad formation, but Dlg and Scrib in the gonadal mesoderm acted critically in the somatic wrapping of the pole cells and the internal structure of the Drosophila embryonic gonads. Dlg also affected the incorporation of the male-specific Sox100B positive mesodermal cells into the male embryonic gonads, yet Sox100B expression in dlg testis remained unaffected. Analysis at later stages revealed that scrib and lgl expression in the somatic lineage of the Drosophila testis, similar to what was previously shown for dlg, was indispensable for testis development and homeostasis, as depletion of these genes resulted in extensive testes defects. The data presented here emphasize the somatic requirement of Scrib, Dlg and Lgl in embryonic gonads, as well as in the Drosophila testis that underlines the importance of the somatic lineage in the establishment and maintenance of testis formation throughout successive developmental stages.

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“…5) Studies of sex-determination mechanisms in invertebrates have primarily focused on insects and nematodes. The Doublesex gene was identified in the fruit fly Drosophila melanogaster (D. melanogaster) as a major sex regulatory gene, 13) and the Mab-3 gene was identified in the nematode Caenorhabditis elegans (C. elegans).…”

Section: De Novo Transcriptome Sequencing To Identify the Sex-determimentioning

confidence: 99%

De novotranscriptome sequencing to identify the sex-determination genes inHyriopsis schlegelii

Shi

Hong

Sheng

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Sex-specific DoublesexM expression in subsets of Drosophilasomatic gonad cells

Cited by 69 publications

References 50 publications

discs large regulates somatic cyst cell survival and expansion in Drosophila testis

discs large regulates somatic cyst cell survival and expansion in Drosophila testis

The internal structure of embryonic gonads and testis development in Drosophila melanogaster requires scrib, lgl and dlg activity in the soma

De novotranscriptome sequencing to identify the sex-determination genes inHyriopsis schlegelii

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