Sex-Specific Gonadal and Gene Expression Changes throughout Development in Fathead Minnow

Leet, Jessica K.; Lesteberg, Kelsey; Schoenfuss, Heiko L.; Olmstead, Allen W.; Amberg, Jon J.; Ankley, Gerald T.; Sepúlveda, Marı́a S.

doi:10.1159/000353877

Cited by 15 publications

(7 citation statements)

References 19 publications

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“…), air‐breathing catfish Claris gariepinus (Raghuveer, Senthilkumaran, Sudhakumari, Sridevi, Rajakumar, Singh, Murugananthkumar & Majumdar ) and fathead minnow (Leet et al . ). Considering the age and maturity state of fish, it seems undifferentiated great sturgeon here was under 6‐month old and the observed differences would be due to the sexual developmental stages difference.…”

Section: Discussionmentioning

confidence: 97%

“…), shovelnose sturgeon Scaphirhynchus platorynchus (Amberg et al . ), Atlantic cod Gadus morhua (Johnsen, Seppola, Torgersen, Delghandi & Andersen ) and fathead minnow Pimephales promelas (Leet, Lesteberg, Schoenfuss, Olmstead, Amberg, Ankley & Sepúlveda ), dmrt1 mRNA expressed in both male and female gonads. Potential forms of dmrt1 have been reported in shovelnose sturgeon Scaphirhynchus platorynchus (Amberg et al .…”

Section: Discussionmentioning

confidence: 99%

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Sex steroid level and sexual dimorphism expression of genes in gonads of the great sturgeonHuso husoLinneaus, 1758 during maturity developmental stages

et al. 2016

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Little is known about molecular mechanisms involved in gonad differentiation in sturgeon species. In non-mammalian vertebrates, sox9, dmrt1, cyp17a1 and ar are male specific genes in testicular differentiation and are highly conserved. In order to understand the mechanism underlying testicular development in sturgeon, we investigated sex steroids level of 11-ketotestosterone (KT) and testosterone (T) and relative expression of sox9, dmrt1, cyp17a1 and ar in great sturgeon gonads during different stages of sexual maturity. The results showed all studied genes had dimorphic patterns in males. In immature gonads (stage I), only cyp17a1 expressed in male gonads, while other genes did not express, and KT and T levels were low. Dmrt1 showed dimorphic expression pattern in male gonads at maturity stage II, III and IV. Furthermore, sox9 and ar mRNA presented significant dimorphic expression pattern in male gonads only at maturity stage 4. Plasma androgens levels were significantly higher in males compared to females during maturity stages II, III and IV. The results showed that among these four genes, only cyp17a1 expressed in male gonads at maturity stage I (immature), suggesting that this gene may be applicable as a sex marker in recently differentiated male great sturgeon. Sexually dimorphic patterns in other studied genes (sox9, dmrt1 and ar) suggesting that these genes may be important for testicular development and differentiation in premature great sturgeon. The obtained results provide a foundation for further research on sex differentiation and developing strategies for the sexing of sturgeon for aquaculture.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Sex steroid level and sexual dimorphism expression of genes in gonads of the great sturgeonHuso husoLinneaus, 1758 during maturity developmental stages

et al. 2016

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“…While Zhang et al (2015) confirmed that sediment was a major source of agrichemical exposure for adult fish, this may not hold true for larvae whose interactions with sediment and water may differ from adult fish. A biological explanation for the absence of defeminization is that the expression profile of estrogen receptors in fathead minnow, namely receptor subtypes α, β and γ, has been shown to vary over the course of ontogeny which would determine what type of response larvae and juvenile fish might have towards an anti-estrogenic mixture (Filby and Tyler 2007;Johns et al 2011;Leet et al 2013). Therefore, while subtle differences were observed between the responses of larvae from the present study and those observed in adult fish from previous studies there is evidence for a consistent response of fish towards agrichemical pulses.…”

Section: Tablementioning

confidence: 99%

Compensatory response of fathead minnow larvae following a pulsed in-situ exposure to a seasonal agricultural runoff event

Ali

Sangster

Snow

et al. 2017

Science of The Total Environment

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Bartelt-Hunt, Shannon L.; and Kolok, Alan S., "Compensatory response of fathead minnow larvae following a pulsed in-situ exposure to a seasonal agricultural runoff event" (2017 H I G H L I G H T S• Fathead minnow larvae were maintained at the Elkhorn River Research Station for an in-situ exposure to a seasonally-occurring runoff.• There was a 1.5-to 13-fold change in waterborne agrichemical contaminants including atrazine, acetochlor and metolachlor.• Peaks in sediment contamination by agrichemicals was discordant with those of waterborne contaminants.• Minnow larvae demonstrated compensation following reduction in size and androgenic gene expression by agrichemical exposure. Agriculturally-dominated waterways such as those found throughout the Midwestern United States often experience seasonal pulses of agrichemical contaminants which pose a potential hazard to aquatic organisms at varying life stages. The objective of this study was to characterize the developmental plasticity of fathead minnow larvae in a natural environment subject to a seasonal episodic perturbation in the form of a complex mixture of agricultural stressors. Fathead minnow larvae were maintained at the Elkhorn River Research Station for a 28-d in situ exposure to an agrichemical pulse event. Minnow larvae were sampled after 14 and 28 days to characterize developmental plasticity through growth measures and relative gene expression. Concentrations of agrichemical contaminants measured in water using polar organic chemical integrative samplers and composite sediment samples throughout the 28-d exposure were quantified using gas chromatography-mass spectrometry. Elevated concentrations of acetochlor, atrazine, and metolachlor were indicative of inputs from agricultural sources and were associated with reductions in body mass, condition factor, and androgenic gene expression in river exposed fathead minnow larvae. However, following a 14-d in situ depuration during the post-pulse period, river exposed larvae overcompensated in previously suppressed biological endpoints. These results indicate that fathead minnow larvae are capable of compensatory responses following episodic exposure to agrichemical stressors. G R A P H I C A L A B S T R A C T a b s t r a c t a r t i c l e i n f o

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“…The samples most likely represent males given that arom has been shown to not be expressed by fathead minnow males through 45 dph. 28,29 Morphometric Assessment. No significant differences were detected in female body mass, GSI (Table S5, ANOVA, p values ≥ 0.25) or length (Table S5, Wilcoxon, p value = 0.14).…”

Section: ■ Resultsmentioning

confidence: 99%

Early Life Stage Exposure to BDE-47 Causes Adverse Effects on Reproductive Success and Sexual Differentiation in Fathead Minnows (Pimephales promelas)

Thornton

Path

Nyström

et al. 2016

Environ. Sci. Technol.

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2,2',4,4'-Tetrabromodiphenyl ether (BDE-47), a compound manufactured for use as a flame retardant, is a ubiquitous environmental contaminant and suspected endocrine disruptor. Though several studies have explored the reproductive effects of BDE-47 in adult fish, there is a paucity of data regarding the reproductive effects of early life stage exposure. The goal of this study was to assess the reproductive effects of early life stage BDE-47 exposure in fathead minnows (Pimephales promelas). To achieve this, minnows were exposed to either a low (57.68 μg BDE-47/g Artemia) or high (392.59 μg BDE-47/g Artemia) dose of BDE-47 from fertilization to 34 days postfertilization (dpf) via a combination of maternal transfer and dietary exposure. Larvae were then raised on a clean diet until sexual maturity (∼184 dpf) when reproductive function was evaluated using a 21 day breeding study. Fish exposed to BDE-47 had significantly reduced clutch size and fecundity relative to controls. BDE-47 exposed groups also had female-biased sex ratios and exposed males had fewer tubercles. Overall, this study demonstrates that exposure to BDE-47 during early life stages can alter both sexual differentiation and reproductive function.

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Sex-Specific Gonadal and Gene Expression Changes throughout Development in Fathead Minnow

Cited by 15 publications

References 19 publications

Sex steroid level and sexual dimorphism expression of genes in gonads of the great sturgeonHuso husoLinneaus, 1758 during maturity developmental stages

Sex steroid level and sexual dimorphism expression of genes in gonads of the great sturgeonHuso husoLinneaus, 1758 during maturity developmental stages

Compensatory response of fathead minnow larvae following a pulsed in-situ exposure to a seasonal agricultural runoff event

Early Life Stage Exposure to BDE-47 Causes Adverse Effects on Reproductive Success and Sexual Differentiation in Fathead Minnows (Pimephales promelas)

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