Sex specific response in cholesterol level in zebrafish ( Danio rerio ) after long-term exposure of difenoconazole

Mu, Xiyan; Wang, Kai; Chai, Tuanyao; Zhu, Li; Yang, Yang; Zhang, Jie; Pang, Sen; Wang, Chengju; Li, Xuefeng

doi:10.1016/j.envpol.2014.11.019

Cited by 72 publications

(28 citation statements)

References 40 publications

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“…Tebuconazole is a triazole fungicide that has been increasingly used in agricultural settings, leading to exposure in humans and wildlife (Toni et al, 2011;Mercadante et al, 2014;Bernab o et al, 2016). Limited studies have been performed so far to evaluate the molecular effects of tebuconazole in male and female species (Slotkin et al, 2008;Mu et al, 2015). In the present study, three developmental stages of zebrafish were used to assess the reproductive and endocrine effects of tebuconazole.…”

Section: Discussionmentioning

confidence: 99%

Endocrine disrupting effects of tebuconazole on different life stages of zebrafish (Danio rerio)

Sun

et al. 2019

Environmental Pollution

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Tebuconazole is a widely used fungicide that has been detected in water ecosystems, of which the concentrations may affect the endocrine function of aquatic organisms. At present study, tissue-specific bioaccumulation of tebuconazole was found in ovary of adult zebrafish, indicating a potential risk of endocrine disruption. In order to evaluate the potential endocrine disrupting effects, three life stages (2 hpf (hours post-fertilization) À60 dpf (days post-fertilization), Stage I; 60e120 dpf, Stage II; 180e208 dpf, Stage III) of zebrafish (Danio rerio) were chronically exposed to tebuconazole at the concentrations ranging from 0.05 mg/L to 1.84 mg/L. Result showed that exposed to tebuconazole could lead to a malebiased sex differentiation in juvenile zebrafish and significant decrease of the percentage of germ cells in sexually-mature zebrafish. Egg production was significantly inhibited by 57.8% and 19.2% after Stage IIand Stage III-exposures, respectively. The contents of 17b-estradiol in gonad decreased by 63.5% when exposed to 0.20 mg/L tebuconazole at Stage II and by 49.5% after exposed to 0.18 mg/L tebuconazole at Stage III, respectively. For all stages exposure, reductions in 17b-estradiol/testosterone ratio were observed, indicating an imbalance in steroids synthesis. Additionally, tebuconazole reduced the expression of cyp19a, which was consistent with the decrease of E2 level. In overall, the present findings indicated that, playing as an anti-estrogen-like chemical, tebuconazole inhibited the expression of Cyp19, thereby impairing steroid hormones biosynthesis, leading to a diminished fecundity of zebrafish.

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Section: Discussionmentioning

confidence: 99%

Endocrine disrupting effects of tebuconazole on different life stages of zebrafish (Danio rerio)

Sun

et al. 2019

Environmental Pollution

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“…The qPCR reactions were carried out using the SsoAdvanced Universal SYBR® Green Supermix (Bio‐Rad) on the Bio‐Rad CFX Connect instrument. Thermocycling conditions used for the qPCR analysis of all genes were as follows: 95 °C for 15 min, followed by 40 cycles of 94 °C for 10 s, 60 °C for 20 s, and 72 °C for 32 s (conditions developed by Mu et al 2015). Data were normalized using ef‐α because its expression is stable throughout zebrafish development (McCurley and Callard 2008), and comparisons of mRNA levels were determined according to the 2 −ΔΔCT method (Livak and Schmittgen 2001).…”

Section: Methodsmentioning

confidence: 99%

“…Primer sequences for hmgcra , hmgcrb , and fdft1 were obtained from Mu et al (2015) and target‐verified by NCBI Primer‐BLAST. All primers were tested for specificity using melt curve analysis and 1.2% agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

Effects of Phenanthrene Exposure on Cholesterol Homeostasis and Cardiotoxicity in Zebrafish Embryos

McGruer

Tanabe

Vliet

et al. 2021

Enviro Toxic and Chemistry

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Polycyclic aromatic hydrocarbons (PAHs) are pervasive pollutants in aquatic ecosystems, and developing fish embryos are especially sensitive to PAH exposure. Exposure to crude oil or phenanthrene (a reference PAH found in oil) produces an array of gross morphological abnormalities in developing fish embryos, including cardiotoxicity. Recently, studies utilizing transcriptomic analyses in several oil-exposed fish embryos found significant changes in the abundance of transcripts involved in cholesterol biosynthesis. Given the vital role of cholesterol availability in embryonic heart development, we hypothesized that cholesterol dysregulation in early development contributes to phenanthrene-induced cardiotoxicity. We exposed zebrafish embryos to 12 or 15 µM phenanthrene from 6 to 72 h post fertilization (hpf) and demonstrated that, in conjunction with pericardial edema and bradycardia, several genes (fdft1 and hmgcra) in the cholesterol biosynthetic pathway were significantly altered. When embryos were pretreated with a cholesterol solution from 6 to 24 hpf followed by exposure to phenanthrene from 24 to 48 hpf, the effects of phenanthrene on heart rate were partially mitigated. Despite changes in gene expression, whole-mount in situ staining of cholesterol was not significantly affected in embryos exposed to phenanthrene ranging in stage from 24 to 72 hpf. However, the 2-dimensional yolk area was significantly increased with phenanthrene exposure at 72 hpf, suggesting that lipid transport from the yolk to the developing embryo was impaired.

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“…Long-term exposure of dietary CrPic led to the up regulation of cyp51, indicating active cholesterol synthesis in P. leopardus. Conversely, other studies have reported that long-term exposure of difenoconazole downregulates the expression of sterol-genesis genes (e.g., cyp51) in zebrafish (Mu et al, 2015). While another study reported that the lss and dhcr24 (24-dehydrocholesterol reductase) gene expressions were up-regulated in the steroid biosynthesis pathway in Nile tilapia (O. niloticus) under salinity stress (Xu et al, 2015).…”

Section: Steroid Biosynthesis Was Up-regulated By Dietary Crpic Exposurementioning

confidence: 91%

Dietary Trivalent Chromium Exposure Up-Regulates Lipid Metabolism in Coral Trout: The Evidence From Transcriptome Analysis

Liu

et al. 2021

Front. Physiol.

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Diet quality greatly affects an animal’s performance and metabolism. Despite the fact that trivalent chromium [Cr(III)] is considered an essential element and is widely used in nutritional supplements for animals and humans, the potential toxicity of Cr(III) is unclear. Here, liver transcriptome sequencing was performed on coral trout (Plectropomus leopardus) exposed to 200 mg kg–1 of dietary organic Cr(III) [as chromium picolinate (CrPic)] for 8 weeks. One-hundred-and thirteen differentially expressed genes (DEGs) were identified in response to Cr(III) stress, in comparison to the control, including 31 up-regulated and 82 down-regulated DEGs. Clusters of Orthologous Groups of proteins (COG) classifies DEGs into 15 functional categories, with the predominant category being related to lipid transport and metabolism (9.73%). The Kyoto Encyclopedia of Genes and Genomes (KEGG) assigned DEGs to six major categories with robust DEGs as part of the lipid metabolism pathway (18.58%). Moreover, KEGG functional enrichment analysis showed that these DEGs are primarily related to steroid biosynthesis, terpenoid backbone biosynthesis, and steroid hormone biosynthesis pathways, of which steroid biosynthesis was the most significant pathway, and 12 key up-regulated DEGs (dhcr7, dhcr24, ebp, lss, msmo1, sqle, cyp51, tm7sf2, sc5dl, fdft1, nsdhl, and hsd17b7) were found for steroid biosynthesis pathways. To validate the RNA sequencing data using quantitative real-time PCR (qRT-PCR), qRT-PCR results indicate that the expression of genes encoding HMGCR, TM7SF2, TRYP2, CTRL, EBP, LSS, and CYP51 were induced, while those encoding THRSP, LCE, and MCM5 were reduced, consistent with RNA-seq results. This findings provides the first evidence that a long-term high dose of Cr(III) intake causes lipid metabolism disorder and potential toxicity in fish. Cautious health risk assessment of dietary Cr(III) intake is therefore highly recommended for the commercial and/or natural diets of aquatic animals, which has previously largely been ignored.

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Sex specific response in cholesterol level in zebrafish ( Danio rerio ) after long-term exposure of difenoconazole

Cited by 72 publications

References 40 publications

Endocrine disrupting effects of tebuconazole on different life stages of zebrafish (Danio rerio)

Endocrine disrupting effects of tebuconazole on different life stages of zebrafish (Danio rerio)

Effects of Phenanthrene Exposure on Cholesterol Homeostasis and Cardiotoxicity in Zebrafish Embryos

Dietary Trivalent Chromium Exposure Up-Regulates Lipid Metabolism in Coral Trout: The Evidence From Transcriptome Analysis

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