Sex-specific spatial and temporal gene expressions of Pheromone biosynthesis activating neuropeptide (PBAN) and binding proteins (PBP/OBP) in Spoladea recurvalis

Abstract

Spoladea recurvalis is one of the most destructive insect pests of amaranth, a leafy vegetable in both Asia and Africa. The present study characterized the pheromone biosynthesis-activating neuropeptide (DH-PBAN) and pheromone/odorant binding proteins in S. recurvalis . The open reading frame of 600 base pairs encodes a 200-amino acid protein possessing five neuropeptide motifs (DH, PBAN, α-, β-, and γ- subesophageal ganglion neuropeptides) and shares a characteristic conserved C-terminal pentapeptide fragment FXPRL. The full-length genome of Spre-DH-PBAN was 4,295 bp in length and comprised of six exons interspersed by five introns. Sequence homology and phylogenetic analysis of Spre-DH-PBAN have high similarity to its homologs in Crambidae of Lepidopteran order. We quantitatively measured the relative expression level (qRT_PCR) of Spre-DH-PBAN gene, the binding proteins such as odorant binding proteins (OBPs) and pheromone binding protein (PBPs) at different developmental stages. The results confirmed their role in recognition and chemoreception of sex pheromone components, and they were distinct, tissue- and sex-specific. This is the first report on the molecular analysis of PBAN gene and binding proteins, which can improve the understanding of molecular mechanisms of growth, development, and reproductive behavior of S. recurvalis , and may become effective targets for controlling this insect.

Identification of neuropeptides and neuropeptide receptor genes in Phauda flammans (Walker)

“…For now, a plethora of neuropeptides and receptors were investigated in insects, such as myoinhibiting peptides (MIPs) 8 – 14 , and so forth. Among these, PBAN, galanin and melanocortin are involved in the control of reproduction 10 , 15 . NPY is regulating feeding and sleep–wake behavior 16 .…”

A comparative protein profile of accessory glands of virgin and matedLeucinodes orbonalismales

“…PBPs are essential components of the insect olfactory system and are vital for recognizing hosts, mating, and oviposition in insects (Zhang et al ., 2012). They belong to a sub‐class of OBPs and are primarily meant to bind and transport sex pheromones in insects (Pelosi et al ., 2006; Liu et al ., 2013; Senthilkumar & Srinivasan, 2019). Evidence has indicated the presence of PBPs/OBPs in MAGs of several insects, including D. melanogaster (Takemori & Yamamoto, 2009) (Findlay et al ., 2008), T. castaneum , H. armigera (South et al ., 2011), and H. assulta (Sun et al ., 2012).…”

Identification and characterisation of PRXamide peptides in the western flower thrips, Frankliniella occidentalis

“…The same peptide was also predicted in the genome of F. occidentalis (Rotenberg et al, 2020). Another 36‐aa PBAN was recently predicted in the moth Spoladea recurvalis , but has not been characterized yet (Senthilkumar & Srinivasan, 2019). Interestingly, the pyrokinin gene of F. occidentalis does not produce another short FSPRL motif that is always found in insect pyrokinin genes (Choi, 2022).…”