Sex steroid application reverses changes in rat castration cells: Unbiased stereological analysis

Nestorović, Nataša; Trifunović, Svetlana; Jarić, Ivana; Manojlović‐Stojanoski, Milica; Ristić, Nataša; Filipović, Branko; Šošić‐Jurjević, Branka; Milošević, Verica

doi:10.2298/abs151201070n

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…In our study estradiol treatment led to inhibition of castration cells, by decreasing intracellular hormone content, volume and volume density of FSH‐ and LH‐containing cells to pre‐Orx levels. However, the volume of pars distalis and number of gonadotropic cells increased as we previously reported (Nestorović et al, ).…”

Section: Discussionsupporting

confidence: 85%

Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study

Nestorović

Trifunović

Manojlović‐Stojanoski

et al. 2018

The Anatomical Record

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The aim of the study was to examine the potential of the principal soy isoflavones, genistein and daidzein, or isoflavone rich soy extract to recover pituitary castration cells in orchidectomized adult male rats in comparison with the effects of estradiol. Two weeks post orchidectomy (Orx), animals received estradiol-dipropionate, genistein, daidzein or soy extract subcutaneously for 3 weeks. Control sham-operated (So) and Orx rats received just the vehicle. Changes in the volumes of pars distalis, of individual follicle-stimulating hormone (FSH) and luteinizing hormone (LH) containing cells, their volume, numerical density and number were determined by unbiased design-based stereology. The intracellular content of βFSH and βLH was estimated by relative intensity of fluorescence (RIF). Orchidectomy increased all examined stereological parameters and RIF. Compared to Orx, estradiol increased the volume of pars distalis, but reversed RIF and all morphometric parameters of gonadotropes to the level of So rats, except their number. Treatments with purified isoflavones and soy extract decreased RIF to the control So level, expressing an estradiol-like effect. However, the histological appearance and morphometrical features of gonadotropes did not follow this pattern. Genistein increased the volume of pars distalis, decreased the volume density of LH-labeled cells and raised the number of gonadotropes. Daidzein decreased the cell volume of gonadotropic cells but increased their number and numerical density. Soy extract induced an increase in number and numerical density of FSH-containing cells. Therefore, it can be concluded that soy phytoestrogens do not fully reverse the Orx-induced changes in pituitary castration cells. Anat Rec, 2018. © 2018 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 85%

Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study

Nestorović

Trifunović

Manojlović‐Stojanoski

et al. 2018

The Anatomical Record

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“…This study suggests the good biocompatibility of novel nanostructured scaffold, which could serve as a suitable candidate for future investigations generally in regenerative medicine. eight, 12,15,19, and 22 days. The obtained values are presented in the diagram depending on the calcium ions concentration.…”

Section: Discussionmentioning

confidence: 98%

“…Stained tissue sections of liver, kidney, and spleen were used to measure the number and volume density (Vv) of epithelial cells. Volume density was calculated through the following formula: Vv = Pf/Pt (mm 0 ), where Pf represents the number of the desired phase on the test system (epithelial cells) and Pt represents the total number of points of the test system [19].…”

Section: Histological and Stereological Analysismentioning

confidence: 99%

Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats

et al. 2020

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Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.

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“…The liver tissue sections stained with H&E were used to measure the numerical density (N v ) and number of hepatocytes per unit volume of hepatocytes and sinusoids. Volume density (V v ) of hepatocytes and sinusoids was calculated through the following formula: V v = P f /P t (mm 0 ), where Pf represents the number of matches on the test system of the desired phase (hepatocyte or lumen of sinusoids) and P t represents the total number of points of the test system (Nestorovi c et al 2016). Micrographs of the liver tissue were acquired in RGB format and converted to binary format for analysing number and numerical density of hepatocytes.…”

Section: Histological and Stereological Analysis Of Liver Tissuesmentioning

confidence: 99%

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

et al. 2019

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Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty‐four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t‐test) and nonparametric tests (Kruskal–Wallis and Mann–Whitney U‐test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.

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Sex steroid application reverses changes in rat castration cells: Unbiased stereological analysis

Cited by 7 publications

References 32 publications

Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study

Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study

Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

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