Sexual Maturation May Affect the Levels of n-6 PUFA in Muscle Tissues of Male Mice

Abstract

Lipid metabolism in mature male mice may be different from immature male mice, but the relationship of lipid metabolism, especially n-6 fatty acid metabolism, and sexual maturation is not clearly established. This study was carried out to elucidate whether sexual maturation may affect the metabolism of functional n-6 fatty acids of lipid components by investigating the composition of fatty acids in the longissimus muscle tissues of mature and immature male mice with GC and analyzing the expression of genes and proteins for synthesis of n-6 fatty acids with real-time PCR and western blotting, respectively. Mature male mice showed significantly higher testosterone level in the sera. Similarly, n-6 fatty acids, levels of linoleic acid (LA 18:2n-6) and total n-6 PUFA (Polyunsaturated fatty acids) were increased, but the levels of γ-linolenic acid (GLA; 18:3n-6), dihomo-γ -linolenic acid (DGLA; 20:3n-6) and arachidonic acid (AA; 20:4 n-6) were decreased in the mature male mice. mRNA levels of Δ 5-desaturase (FASD1) and elongase (ELOVL5) genes related to n-6 fatty acid metabolism increased. However, the level of FADS1 protein only increased in mature male mice. In conclusion, this study suggested that sexual maturation of male mice affected n-6 fatty acid metabolism by stimulating the expression of enzyme FADS1 of n-6 PUFA metabolism.

On the performance of tests for the detection of signatures of selection: a case study with the Spanish autochthonous beef cattle populations

“…There are several other interesting signatures of selection such as those located on BTA27 (between 36,466,580 and 40,862,444 bp) and BTA28 (between 41,643,416 and 45,215,488 bp) for the ANI and Mo populations, respectively. The first genomic region includes the IKBKB ( inhibitor of kappa light polypeptide gene enhancer in B - cells, kinase beta ), DKK4 ( dickkopf WNT signaling pathway inhibitor 4 ) and VDAC3 ( voltage - dependent anion - selective channel protein 3 ) genes that are associated with immune response to trypanosoma infection in African populations [56], and the second region contains the ALOX5 ( arachidonate 5 - lipoxygenase ) and RASSF4 ( ras association ( RalGDS/AF - 6 ) domain family member 4 ) genes, that are related with growth [57] and feed conversion [58], respectively.…”

Effects of Supplementary Kelp Feeding on the Growth, Gonad Yield, and Nutritional and Organoleptic Quality of Subadult Sea Urchin (Strongylocentrotus intermedius) with Soya Lecithin Intake History

“…The highest levels of estradiol-17β were found in the blood of sexually mature females striped catfish (Pangasianodon hypophthalmus) [68]. Sexual maturation of male mice stimulated the expression of enzyme FADS1, a key rate-limiting enzyme of ARA synthesis from its substrate LA [69]. Thus, it is essential to compare the fatty acids and investigate related gene expression at different maturity stages of gonads in the following studies to better understanding the LC-PUFA synthesis-related mechanisms.…”