Sexual specific functions of Tub1 beta‐tubulins require stage‐specific RNA processing and expression in <i>Fusarium graminearum</i>

Chen, Daipeng; Wu, Chunlan; Hao, Chaofeng; Huang, Panpan; Liu, Huiquan; Bian, Zhuyun; Xu, Jin‐Rong

doi:10.1111/1462-2920.14441

Cited by 9 publications

(6 citation statements)

References 60 publications

(111 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, with the addition of the 3′-UTR, FgCSN12 successfully complemented the defects in ascosporogenesis, indicating that the function of FgCSN12 3′UTR sequences is only required during sexual reproduction. A similar phenomenon was also observed in complementation assays for Fgama1 and Fgtub1 mutants [ 17 , 20 ]. These genes may have stage-specific transcription termination or alternative polyadenylation sites (APAs) in ascogenous tissues.…”

Section: Discussionsupporting

confidence: 76%

“…In addition, although the highly similar cyclin-dependent kinase (CDK) and beta-tubulin genes functionally overlap during the asexual stage, only CDC2A and TUB1 play an important role during ascosporogenesis [ 18 , 19 , 20 ]; therefore, there are differences in the regulation of the cell cycle and microtubule cytoskeleton between asexual development and sexual reproduction.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus Fusarium graminearum

Jiang

Zhang

Wang

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Fusarium head blight (FHB), caused by the fungal pathogen Fusarium graminearum, is a destructive disease worldwide. Ascospores are the primary inoculum of F. graminearum, and sexual reproduction is a critical step in its infection cycle. In this study, we characterized the functions of FgCsn12. Although the ortholog of FgCsn12 in budding yeast was reported to have a direct interaction with Csn5, which served as the core subunit of the COP9 signalosome, the interaction between FgCsn12 and FgCsn5 was not detected through the yeast two-hybrid assay. The deletion of FgCSN12 resulted in slight defects in the growth rate, conidial morphology, and pathogenicity. Instead of forming four-celled, uninucleate ascospores, the Fgcsn12 deletion mutant produced oval ascospores with only one or two cells and was significantly defective in ascospore discharge. The 3′UTR of FgCsn12 was dispensable for vegetative growth but essential for sexual reproductive functions. Compared with those of the wild type, 1204 genes and 2240 genes were up- and downregulated over twofold, respectively, in the Fgcsn12 mutant. Taken together, FgCsn12 demonstrated an important function in the regulation of ascosporogenesis in F. graminearum.

show abstract

Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus Fusarium graminearum

Jiang

Zhang

Wang

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…To determine whether deletion of FgPAL1 affects cytokinesis and nuclear distribution, we transformed an H1‐GFP fusion construct (Chen et al ., 2018) into PH‐1 and the Fgpal1 mutant M8. When the resulting transformants were examined by epifluorescence microscopy, most of the wild‐type conidia (76.3 ± 25.4%) had one nucleus in each compartment (Fig.…”

Section: Resultsmentioning

confidence: 99%

FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum

Yin

Hao

Niu

et al. 2020

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Ascospores are the primary inoculum in Fusarium graminearum, a causal agent of wheat head blight. In a previous study, FgPAL1 was found to be upregulated in the Fgama1 mutant and important for ascosporogenesis. However, the biological function of this well-conserved gene in filamentous ascomycetes is not clear. In this study, we characterized its functions in growth, differentiation and pathogenesis. The Fgpal1 mutant had severe growth defects and often displayed abnormal hyphal tips. It was defective in infectious growth in rachis tissues and spreading in wheat heads. The Fgpal1 mutant produced conidia with fewer septa and more nuclei per compartment than the wild type. In actively growing hyphal tips, FgPal1-GFP mainly localized to the subapical collar and septa. The FgPal1 and LifeAct partially co-localized at the subapical region in an interdependent manner. The Fgpal1 mutant was normal in meiosis with eight nuclei in developing asci but most asci were aborted. Taken together, our results showed that FgPal1 plays a role in maintaining polarized tip growth and coordination between nuclear division and cytokinesis, and it is also important for infectious growth and developments of ascospores by the free cell formation process.

show abstract

“…Interestingly, the Δ TRR mutants were defective in ascus and ascospore development but produced protoperithecia on CA plates. Like TUB1 in F. graminearum (Chen et al, 2018), FgTRR is dispensable for initial stages of perithecium formation. Similarly, FgNoxR, a regulatory subunit of NADPH oxidases, is essential for perithecium formation in F. graminearum (Zhang C.K.…”

Section: Discussionmentioning

confidence: 99%

Thioredoxin Reductase Is Involved in Development and Pathogenicity in Fusarium graminearum

Fan

Ding

et al. 2019

Front. Microbiol.

View full text Add to dashboard Cite

Fusarium graminearum is one of the causal agents of Fusarium head blight and produces the trichothecene mycotoxin, deoxynivalenol (DON). Thioredoxin reductases (TRRs) play critical roles in the recycling of oxidized thioredoxin. However, their functions are not well known in plant pathogenic fungi. In this study, we characterized a TRR orthologue FgTRR in F. graminearum. The FgTRR-GFP fusion protein localized to the cytoplasm. FgTRR gene deletion demonstrated that FgTRR is involved in hyphal growth, conidiation, sexual reproduction, DON production, and virulence. The ΔTRR mutants also exhibited a defect in pigmentation, the expression level of aurofusarin biosynthesis-related genes was significantly decreased in the FgTRR mutant. Furthermore, the ΔTRR mutants were more sensitive to oxidative stress and aggravated apoptosis-like cell death compared with the wild type strain. Taken together, these results indicate that FgTRR is important in development and pathogenicity in F. graminearum.

show abstract

Sexual specific functions of Tub1 beta‐tubulins require stage‐specific RNA processing and expression in Fusarium graminearum

Cited by 9 publications

References 60 publications

FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus Fusarium graminearum

FgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus Fusarium graminearum

FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum

Thioredoxin Reductase Is Involved in Development and Pathogenicity in Fusarium graminearum

Contact Info

Product

Resources

About