Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus

Abstract

Dynein axonemal light intermediate chain 1 (dnali1) is an important part of axonemal dyneins and plays an important role in the growth and development of animals. However, there is little information about dnali1 in sh. Herein, we cloned dnali1 gene from the genome of olive ounder (Paralichthys olivaceus), a commercially important maricultured sh in China, Japan, and Korea, and analyzed its expression patterns in different gender sh. The DNA sequence of the entire gene contained a 771 bp open reading frame (ORF), two different sizes of 5' untranslated region (5'UTR), and a 1499 bp 3' untranslated region (3'UTR). Two duplicated 922 nt fragments were found in dnali1 mRNA. The rst fragment contained the downstream coding region and the front portion of 3'UTR, and the second fragment was entirely located in 3'UTR. Multiple alignments indicated that the ounder Dnali1 protein contained the putative conserved domain. Its expression showed sexually dimorphic with predominant expression in the ounder testis, and lower expression in other tissues. The gene with the larger 5'UTR was speci cally expressed in the testis. The highest expression level in the testis was detected at stages and . Transient expression analysis showed that the 922 bp repeated sequence 3'UTR of dnali1 down-regulated the expression of GFP at the early stage in zebra sh. The results implied that dnali1 might play an important role in ounder testis, especially in the period of spermatogenesis, and the 5'UTR and the repetitive sequences in 3'UTR might contain some special regulatory elements for the cilia.

DNALI1 interacts with the MEIG1/PACRG complex within the manchette and is required for proper sperm flagellum assembly in mice

“…Recently, Dnali1 was identified as a sex-related gene involved in spermatogenesis of several fishes, including the Odontobutis potamophila , the Ussuri catfish Pseudobagrus ussuriensis , and the olive flounder Paralichthys olivaceus ( Wang et al, 2019 ; Pan et al, 2021 ; Wang et al, 2021 ). The expression of Dnali1 showed sexual dimorphism with predominant expression in the testis.…”

“…The expression of Dnali1 showed sexual dimorphism with predominant expression in the testis. This suggested that the fish Dnali1 might play an important role in the testis, especially in the period of spermatogenesis ( Wang et al, 2021 ). Sajid et al cloned the murine Dnali1 with significant similarity to the p28 gene of Chlamydomonas reinharditii and to DNALI1 ( hp28 ) gene ( Rashid et al, 2006 ).…”

MEIG1/PACRG associated and non-associated functions of axonemal dynein light intermediate polypeptide 1 (DNALI1) in mammalian spermatogenesis

“…Recently, Dnali1 was identified as a sex-related gene involved in spermatogenesis of several fishes, including the Odontobutis potamophila, the Ussuri catfish Pseudobagrus ussuriensis, and the olive flounder Paralichthys olivaceus (19)(20)(21). The expression of Dnali1 showed sexually dimorphism with predominant expression in the testis.…”

“…The expression of Dnali1 showed sexually dimorphism with predominant expression in the testis. This suggested that the fish Dnali1 might play an important role in the testis, especially in the period of spermatogenesis (21). Sajid et al cloned the murine Dnali1 with significant similarity to the p28 gene of Chlamydomonas reinharditii and to DNALI1 ( hp28 ) gene (22).…”