Shape-dependent internalization kinetics of nanoparticles by membranes

Chen, Liping; Xiao, Shiyan; Zhu, Hong; Wang, Lei; Liang, Haojun

doi:10.1039/c5sm01869b

Cited by 76 publications

(75 citation statements)

References 58 publications

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“…On the other hand, if a high cellular uptake is desired, spherical polymersomes, which require the least energy for endocytosis as compared to non-spherical ones of the same volume, may be more desirable. [80] …”

Section: Controlling Polymersome Shapementioning

confidence: 99%

Engineering Polymersomes for Diagnostics and Therapy

Leong

Teo

Aakalu

et al. 2018

Adv Healthcare Materials

113

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Engineered polymer vesicles, termed as polymersomes, confer a flexibility to control their structure, properties, and functionality. Self-assembly of amphiphilic copolymers leads to vesicles consisting of a hydrophobic bilayer membrane and hydrophilic core, each of which is loaded with a wide array of small and large molecules of interests. As such, polymersomes are increasingly being studied as carriers of imaging probes and therapeutic drugs. Effective delivery of polymersomes necessitates careful design of polymersomes. Therefore, this review article discusses the design strategies of polymersomes developed for enhanced transport and efficacy of imaging probes and therapeutic drugs. In particular, the article focuses on overviewing technologies to regulate the size, structure, shape, surface activity, and stimuli- responsiveness of polymersomes and discussing the extent to which these properties and structure of polymersomes influence the efficacy of cargo molecules. Taken together with future considerations, this article will serve to improve the controllability of polymersome functions and accelerate the use of polymersomes in biomedical applications.

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Section: Controlling Polymersome Shapementioning

confidence: 99%

Engineering Polymersomes for Diagnostics and Therapy

Leong

Teo

Aakalu

et al. 2018

Adv Healthcare Materials

113

View full text Add to dashboard Cite

show abstract

“…39,51,58 Through this method, we obtained a lipid membrane with a constant membrane tension, which is about 1.9 Â 10 À2 pN nm À1 (ESI †). We utilized the velocity-Verlet algorithm to perform time integration, and a Langevin thermostat to control the system temperature at k B T = 1.1e with a friction constant G = t À1 , where k B is the Boltzmann constant and T is the temperature.…”

Section: Model and Methodsmentioning

confidence: 99%

“…The kinetics of endocytosis is similar to that of recent molecular simulation studies. 10,14,51 When the diameter of the spherical NPs is reduced to 8s, individual NPs cannot be completely wrapped by the lipid membrane, and the wrapping fraction of the NPs is about 0.85 ( Fig. 2b and 3a).…”

Section: View Article Onlinementioning

confidence: 99%

“…The internalization process is accompanied by orientation changes of the NPs, which is consistent with the previous theoretical prediction and numerical simulations. 22,51 For a small single oblate NP (D v = 8s), it lies flat on the membrane and the wrapping fraction of the NP is 0.55 ( Fig. 7b and 8a).…”

Section: Wrapping Of Multiple Oblate Npsmentioning

confidence: 99%

“…In fact, experimental and theoretical studies have shown that local geometrical properties such as the local mean curvature of NPs would significantly affect the wrapping behavior of lipid membranes on NP surfaces. 11,25,51 For example, the local mean curvature of NPs at the point of initial contact with macrophages determines whether the cells internalize or simply spread on NPs. 11 For oblate NPs, Weikl et al predicted that the membrane forms a tubular structure, in which the more strongly curved edges of the neighboring NPs face each other, to wrap multiple NPs.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cooperative wrapping of nanoparticles of various sizes and shapes by lipid membranes

et al. 2017

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Understanding the interaction between nanoparticles (NPs) and cell membranes is crucial for the design of NP-based drug delivery systems and for the assessment of the risks exerted by the NPs. Recent experimental and theoretical studies have shown that cell membranes can mediate attraction between NPs and form tubular structures to wrap multiple NPs. However, the cooperative wrapping process is still not well understood, and the shape effect of NPs is not considered. In this article, we use largescale coarse-grained molecular dynamics (CGMD) simulations to study the cooperative wrapping of NPs when a varying number of NPs adhered to the membrane. Spherical, prolate and oblate NPs of different sizes are considered in this study. We find that, in addition to tubular structures, the membrane can form a pocket-like and a handle-like structure to wrap multiple NPs depending on the size and shape of the NPs. Furthermore, we find that NPs can mediate membrane hemifusion or fusion during this process.Our findings provide new insights into the interaction of NPs with the cell membrane.

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Endocytosis‐Enabled Construction of Silica Nanochannels Crossing Living Cell Membrane for Transmembrane Drug Transport

Pan

Tang

et al. 2020

Adv Funct Materials

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Artificial transmembrane channel (ATC) analogs are developed for overcoming biological membrane barriers and realizing transmembrane drug delivery, which are mostly studied within artificial lipid bilayers and thus lacked enough stability in practical applications on living cells. Here, natural endocytosis of silica‐based 1D nanomaterials (nanowires) with an ultrahigh aspect ratio is investigated. Enlightened by partially endocytosed ultralong silica nanowires, ATC that can penetrate living cell membranes for transmembrane transportation of small drug molecules is creatively constructed, resulting in enhanced drug delivery efficacy and decreased the half maximal inhibitory concentration. For the first time, an in‐depth study of the cellular uptake of 1D nanomaterials with ultrahigh aspect ratios (from 10 to 120) into living cells is carried out. Through confocal laser scanning microscopy observation, the endocytosis process of ultralong nanowires, including full uptake of short nanowires and partial uptake of longer nanowires, is clarified. Theoretical simulation is performed to give a fundamental understanding on the endocytosis mechanism of ultralong 1D silica nanowires. The simulation results demonstrate the time‐dependent internalization dynamics of the nanowires, which agrees well with our experimental results. This work not only clarifies the cellular interaction between 1D nanomaterials and living cells, but also pioneers the use of natural endocytosis of 1D nanomaterials for constructing ATC.

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Shape-dependent internalization kinetics of nanoparticles by membranes

Cited by 76 publications

References 58 publications

Engineering Polymersomes for Diagnostics and Therapy

Engineering Polymersomes for Diagnostics and Therapy

Cooperative wrapping of nanoparticles of various sizes and shapes by lipid membranes

Endocytosis‐Enabled Construction of Silica Nanochannels Crossing Living Cell Membrane for Transmembrane Drug Transport

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