Shea Nut Oil Extracts Enhance the Intra-Articular Sodium Hyaluronate Effectiveness on Surgically Induced OA Progression in Rats

Abstract

Osteoarthritis (OA) progression is associated with joint pain and stiffness. Intra-articular hyaluronic acid (IAHA) injection in knee OA restores the viscoelasticity of the joint and prevents cartilage damage. Shea nut oil extract (SNO) was shown to provide chondroprotection on surgically-induced OA progression in rats. Here we aim to examine IAHA injection supplemented with SNO diet for a synergetic evaluation on the disease progression in OA rats. We employed an anterior cruciate ligament transection plus medial meniscectomy-induced knee OA rat model with up to 12 weeks of sign/behavior observation (knee width, weight-bearing) and histological assessments of joint damage. We found both IAHA and SNO alone significantly attenuated histological changes of cartilage degeneration and synovial reactions in these knee OA rats. Nonetheless, oral SNO alone mitigated OA pain and inflammation while IAHA alone had no significant impact on the weight-bearing test and knee joint swelling. Moreover, with IAHA-treated rats fed with oral SNO diet, additional anti-inflammatory and anti-nociceptive effects were found, which further enhanced and maintained IAHA protection. Given the differential phenotype of oral SNO vs. IAHA, a regimen of IAHA coupled with SNO supplement provides a long-term effect of IAHA treatment. Taken together, the SNO supplement can be safely used as an adjuvant diet for chronic symptomatic relief of OA coupled with IAHA management.

Vitamin D reduces pain and cartilage destruction in knee osteoarthritis animals through inhibiting the matrix metalloprotease (MMPs) expression

“…OA was induced in rats, as previously reported [ [29] , [30] , [31] ]. To induce OA in rats, the ACLT + MMx method was used on the right knee.…”

Detection and Evaluation of Serological Biomarkers to Predict Osteoarthritis in Anterior Cruciate Ligament Transection Combined Medial Meniscectomy Rat Model

“…OA induction was carried out by following the previous reports [ 16 , 17 , 18 , 19 ]. The animal experiments were conducted according to institutional and international standards of ethics (Principles of Laboratory Animal Care, National Institutes of Health) and approved by the Institutional Animal Care and Use Committee (IACUC) of Cathay General Hospital (Taipei, Taiwan).…”

“…The stained sections were scanned using a Slide Scanner ZEISS Axio Scan Z1 image system (Jena, Germany). The mean cartilage degeneration score (determined using the modified Osteoarthritis Research Society International scoring system) [ 16 , 17 , 18 , 19 ] and the mean cartilage thickness on the medial tibial plateau were evaluated using ZEN lite 3.2 software (Carl Zeiss Microscopy GmbH, Jena, Germany).…”

“…OA is induced by various methods including anterior cruciate ligament transection (ACLT), medial meniscectomy (MMx), medial meniscus tear, ovariectomy, and combinational models. Among all combinational models, the anterior cruciate ligament transection combined with the medial meniscectomy (ACLT+MMx) model is preferred due to its reproducibility and rapid effect on OA progression [ 16 , 17 , 18 , 19 ]. The fast induction of OA helps in studying the effectiveness of drugs earlier than other reported OA models [ 20 ].…”

Combination of fenchone and sodium hyaluronate ameliorated constipation‐predominant irritable bowel syndrome and underlying mechanisms

“…It is primarily present in the vitreous body of the eye, skin, synovial fluid, and skeletal tissues (L. Hu et al., 2022). Sodium hyaluronate is an intrinsic component within the knee joint and provides viscoelastic properties to synovial fluid (Chen & Wong, 2020). Sodium hyaluronate is an iconic cosmetic active ingredient, owing to its multiple physiological roles in promoting hydration, and improving viscoelasticity and tonicity (Meunier et al., 2022).…”