Shedding of vesicular material from the cell surface of eukaryotic cells: different cellular phenomena

Beaudoin, Adrien R.; Grondin, Gilles

doi:10.1016/0304-4157(91)90014-n

Cited by 124 publications

(89 citation statements)

References 199 publications

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“…One possible origin of soluble NEP is by the shedding process, which occurs in many eukaryotic cells with membrane-bound proteins being released with portions of plasma membrane or as proteolipid aggregates. 32 The soluble form of NEP found in plasma could, therefore, originate from the release of the entire membrane NEP by leakage in alveolar epithelial cells or activated neutrophils. A post-translational proteolytic cleavage, as observed in the case of ACE, 33 cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

Neutral endopeptidase and angiotensin I converting enzyme insertion/deletion gene polymorphism in humans

Oliveri

et al. 2004

J Hum Hypertens

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Neutral endopeptidase (NEP) hydrolyses angiotensins (Ang) I and II and generates angiotensin-(1-7) ]. In humans, the insertion/deletion (I/D) angiotensin-I converting enzyme (ACE) gene polymorphism determined plasma ACE levels by 40%. In rats, a similar polymorphism determines ACE levels which are inversely associated to NEP activity. The objective of this study is to evaluate the relationship between ACE expression and plasma NEP activity in normotensive subjects and in hypertensive patients. In total, 58 consecutive patients with hypertension, evaluated in our Hypertension Clinic, were compared according to their ACE I/D genotypes with 54 control subjects in terms of both plasma ACE activity and NEP activities. Plasma ACE activity was elevated 51 and 70% in both DD ACE groups (normotensives and hypertensives) compared with their respective ID and II ACE groups (Po0.001). A significant effect of the ACE polymorphism and of the hypertensive status on ACE activity was observed (Po0.001). In normotensive DD ACE subjects, NEP activity was 0.30 7 0.02 U/ml, whereas in the normotensive II ACE and in the normotensive ID ACE subjects NEP activity was increased 65 and 48%, respectively (Po0.001). In the hypertensive DD ACE patients, NEP activity was 0.47 7 0.03 U/mg. An effect of the I/D ACE genotypes on NEP activity (Po0.04) and an interaction effect between the I/D ACE genotype and the hypertensive status were also observed (Po0.001). These results are consistent with a normal and inverse relationship between the ACE polymorphism and NEP activity in normotensive humans (as is also observed in rats). This normal relationship is not observed in hypertensive patients.

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Section: Discussionmentioning

confidence: 99%

Neutral endopeptidase and angiotensin I converting enzyme insertion/deletion gene polymorphism in humans

Oliveri

et al. 2004

J Hum Hypertens

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“…[27][28][29] The other form of B10 in bile has a smaller molecular mass than the form present in serum. Shedding 30 can cause the release of membrane proteins from plasma membranes; however, removing membrane fragments from cells would generate circulating forms with the same apparent molecular mass as did the membranous form on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 5-kd diminution in apparent molecular mass of the serum enzyme relative to the cellular enzyme suggests that a small portion of the protein was removed upon formation of the serum enzyme; this is consistent with a cleavage occurring immediately after the transmembrane domain.…”

Section: Discussionmentioning

confidence: 99%

Identification of B10, an alkaline phosphodiesterase of the apical plasma membrane of hepatocytes and biliary cells, in rat serum: Increased levels following bile duct ligation and during the development of cholangiocarcinoma

et al. 1998

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Alkaline phosphodiesterase (APDE) is associated with the cellular plasma membrane of many organs. Several isoforms are also detected in normal human serum and their respective amounts vary in liver diseases but their significance is unknown. The aims of this study were: 1) to identify a serum form of B10, an APDE exclusively localized at the apical pole of the plasma membrane of rat hepatocytes and biliary cells; 2) to gain insight into its origin; and 3) to investigate its behavior, in two liver diseases in which an abnormal membrane expression of B10 has been reported, namely cholestasis and cholangiocarcinoma. A soluble form of B10 was immunoprecipitated from normal rat serum, which amounted to 13% of total serum APDE activity. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the size of the serum enzyme was 125 kd, which is slightly lower than that found in the plasma membrane (130 kd). In bile, a 120-kd and a 130-kd form was found. A sixfold and fivefold increase of B10 APDE activity was observed in the serum of bile duct-ligated rats and in the Long-Evans Cinnamon (LEC) rats which spontaneously develop cholangiocarcinoma. The molecular size of the form present in serum was unchanged. A threefold increase was also observed in LEC rats which had not yet developed a cholangiocarcinoma. In conclusion, we identified a soluble form of B10 in normal rat serum. The increase in serum B10 in the experimental and pathological conditions investigated does not seem to result from passage of the biliary form to the serum but seems to be caused by increased cleavage of the membrane form. Its rise early during the onset of cholangiocarcinoma suggests that B10 in the serum might be a marker of carcinogenesis and/or be involved in the development of cholangiocarcinoma.

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“…Furthermore, it has been recently shown that cells may transiently modify the phenotype of neighboring cells by transferring surface receptors, intracellular proteins, and mRNA in mechanisms that involve exchange of cell-membrane-derived microvesicles (Ratajczak et al, 2006a,b). Shedding of membrane-derived microvesicles is a physiological phenom-enon that accompanies cell growth and cell activation, for example, hypoxia or oxidative injury (Beaudoin and Grondin, 1991;VanWijk et al, 2003;Morel et al, 2004;Hugel et al, 2005 -galactosidase).…”

Section: Bm-derived Stem Cells and Tissue/organ Regeneration: Evidencmentioning

confidence: 99%

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

Kucia

Wan

Ratajczak

2007

Developmental Dynamics

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Data from our and other laboratories provide evidence that bone marrow (BM) contains a population of stem cells that expresses early developmental markers such as (1) stage-specific embryonic antigen (SSEA) and (2) transcription factors Oct-4 and Nanog. These are the markers characteristic for embryonic stem cells, epiblast stem cells, and primordial germ cells (PGC). The presence of these stem cells in adult BM supports the concept that this organ contains some population of pluripotent stem cells that is deposited in embryogenesis during early gastrulation. We hypothesize that these cells could be direct descendants of the germ lineage that, to pass genes on to the next generations, has to create soma and, thus, becomes a "mother lineage" for all somatic cell lineages present in the adult body. Germ potential is established after conception in totipotent zygotes and retained in blastomeres of morula, cells from the inner cell mass of blastocyst, epiblast, and population of PGC. We will present a concept that SSEA ؉ Oct-4 ؉ Nanog ؉ cells identified in BM could be descendants of epiblast cells as well as some rare migrating astray PGC. Developmental Dynamics 236:3309 -3320, 2007.

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Shedding of vesicular material from the cell surface of eukaryotic cells: different cellular phenomena

Cited by 124 publications

References 199 publications

Neutral endopeptidase and angiotensin I converting enzyme insertion/deletion gene polymorphism in humans

Neutral endopeptidase and angiotensin I converting enzyme insertion/deletion gene polymorphism in humans

Identification of B10, an alkaline phosphodiesterase of the apical plasma membrane of hepatocytes and biliary cells, in rat serum: Increased levels following bile duct ligation and during the development of cholangiocarcinoma

Bone marrow‐derived very small embryonic‐like stem cells: Their developmental origin and biological significance

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