Shewanella maritima sp. nov., a facultative anaerobic marine bacterium isolated from seawater, and emended description of Shewanella intestini

Abstract

A Gram-stain-negative, motile, facultative anaerobic rod-shaped marine bacterium, designated strain D4-2T, was isolated from a sample of seawater collected at Dong-do marina, Dokdo Island, in the East Sea of the Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain D4-2T was affiliated with members of genus Shewanella and closely related to Shewanella intestini XMDDZSB0408T (97.4%), followed by Shewanella gelidii RZB5-4T (96.7 %) and Shewanella inventionis KX27T (96.1 %). D4-2T has a single circular chromosome of 4.72 Mbp with a DNA G+C content of 44.5 mol%. Average nucleotide identity (ANI) and in silico DNA–DNA hybridization (DDH) values between strain D4-2T and the previously mentioned type strains of species of the genus Shewanella were in range of 69–83.8 % and 20.5–21.7 %, respectively. Growth was observed at 10–36 °C (optimum 29–32 °C), at pH 6–9 (optimum pH 7), and with 1–6% NaCl (optimum 2%). The predominant fatty acids (>10 %) of D4-2T were iso-C15:0 and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The respiratory quinones were Q-7, Q-8, MK-7 and MMK-7. Phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified aminolipid and four unidentified lipids were detected in D4-2T. On the basis of phenotypic, chemotaxonomic and molecular properties, D4-2T represents a novel species of the genus Shewanella , for which the name Shewanella maritima sp. nov. is proposed with D4-2T as the type strain (=KCTC 72040T=JCM 33294T).

Shewanella nanhaiensis sp. nov., a marine bacterium isolated from sediment of South China Sea, and emended descriptions of Shewanella woodyi, Shewanella hanedai and Shewanella canadensis

“…S3). Phosphatidylethanolamine and phosphatidylglycerol were the major and common polar lipids of strain NR704-98 T , S. woodyi MS32 T and S. hanedai 281 T , which was consistent with profiles previously reported for Shewanella species [45,46]. Strains NR704-98 T and S. woodyi MS32 T contained one unidentified aminophospholipid that was absent in S. hanedai 281 T .…”

Three novel marine species of the genus Reichenbachiella exhibiting degradation of complex polysaccharides

“…The optimal temperature and salt tolerance for the growth was determined by using MA ( Muhammad et al, 2022 ) while the optimal pH for the growth was tested in MB ( Muhammad et al, 2023 ). To determine the growth under an anaerobic condition, all three strains were tested on a solid medium composed of 0.2% (w/v) glucose, 2% (w/v) NaCl, 0.1% (w/v) polypeptone, 0.1% (w/v) KH 2 PO 4, 0.1% (w/v) K 2 HPO 4 , 0.2% (w/v) NH 4 Cl, 0.14% (w/v) MgSO4, NaNO 3 (w/v) 0.17, 1.5% (w/v) agar, 0.1% (w/v) resazurin, 0.05% (w/v) cysteine HCl, 1 mL trace elements, and 1 mL multivitamins ( Wolin et al, 1963 ; Kim et al, 2001 ; Bae et al, 2020 ).…”