Shewanella pacifica sp. nov., a polyunsaturated fatty acid-producing bacterium isolated from sea water

Ivanova, E. P.; Gorshkova, N. M.; Bowman, J. P.; Lysenko, A. M.; Zhukova, N. V.; Sergeev, A. F.; Mikhailov, V. V.; Nicolau, D. V.

doi:10.1099/00207713-54-6-2441-a

Cited by 11 publications

(16 citation statements)

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“…Ivanova et al (2003) reported and characterized new bacteria of the genus Shewanella (Shewanella waksmanii) isolated from the marine sipuncula. In other investigation, Ivanova et al (2004) characterized Shewanella pacifica, a polyunsaturated fatty acid-producing bacterium isolated from seawater.…”

Section: Discussionmentioning

confidence: 98%

Jania rubens-associated bacteria: molecular identification and antimicrobial activity

et al. 2011

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Marine macroalgae surfaces constitute suitable substrata for bacterial colonization which are known to produce bioactive compounds. Thus, hereby we focused on heterotrophic aerobic bacteria species associated with coralline red alga Jania rubens (northern coast of Tunisia, southern Mediterranean Sea) and their inhibition against several microbial marine and terrestrial species. The whole collection (19 isolates, J1 to J19) was identified, based on their 16S ribosomal RNA gene sequences as Proteobacteria (14 strains), Bacteroidetes (4 strains) and Firmicutes (1 strain). Thirty-six percent of the isolates (J2, J9, J11, J13, J16, J17 and J18) were antibiotic-like producers with in vitro inhibition against Gram + and Gram − bacteria and the yeast Candida albicans. Highest level of inhibition was revealed for the isolates J2, J9 and J13 identified respectively as Bacillus, Aquimarina and Pseudomonas, with strong activity against Staphylococcus aureus, Micrococcus and C. albicans, with inhibition diameters of 25 to 35 mm shown by drop test assay on T soy agar plates. Furthermore, we tested inhibition of J. rubens crude organic extracts against human and marine bacteria as well as against all J. rubens isolates, in order to determine the degree of affinity of the epibionts to their proper host. The recovery of strains with antimicrobial activity suggests that J. rubens represent an ecological niche which harbors a specific microbial diversity worthy of further secondary metabolites investigation.

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Section: Discussionmentioning

confidence: 98%

Jania rubens-associated bacteria: molecular identification and antimicrobial activity

et al. 2011

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“…The carnitine levels in soil and water may vary depending on the bacterial flora at the site and whether the bacteria inhabiting those environments are capable of carnitine metabolism. The presence and utility of carnitine in the environment is supported, in part, by the number of bacteria capable of carnitine metabolism, including a few newly identified species such as Burkholderia caribensis (Achouak et al, 1999), Bacillus decisifrondis (Zhang et al, 2007), Pseudomonas kilonensis (Sikorski et al, 2001) from soil, and Shewanella pacifica from sea water (Ivanova et al, 2004).…”

Section: Carnitine In the Environmentmentioning

confidence: 99%

Carnitine in bacterial physiology and metabolism

2015

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Carnitine is a quaternary amine compound found at high concentration in animal tissues, particularly muscle, and is most well studied for its contribution to fatty acid transport into mitochondria. In bacteria, carnitine is an important osmoprotectant, and can also enhance thermotolerance, cryotolerance and barotolerance. Carnitine can be transported into the cell or acquired from metabolic precursors, where it can serve directly as a compatible solute for stress protection or be metabolized through one of a few distinct pathways as a nutrient source. In this review, we summarize what is known about carnitine physiology and metabolism in bacteria. In particular, recent advances in the aerobic and anaerobic metabolic pathways as well as the use of carnitine as an electron acceptor have addressed some long-standing questions in the field. Received 5 January 2015 Accepted 17 March 2015Introduction Carnitine (c-trimethylamino-b-hydroxybutyric acid) ( Fig. 1) is a quaternary amine compound that can be produced by all domains of life, and was discovered in muscle extract in 1905 by Gulewitsch & Krimberg (1905) and Kutscher (1905). It was shown to be essential for larval development of the mealworm Tenebrio molitor and was originally designated vitamin B T based on this requirement. Later, it was discovered that carnitine can be synthesized in mammals and is now considered to be a quasi-nutrient or conditionally essential nutrient (Flanagan et al., 2010), as neonates have reduced biosynthesis and rely on placental transfer of carnitine in utero and exogenous sources after birth (Combs, 2012). Fifty years after the discovery of carnitine, it was demonstrated that assorted Gram-positive and Gram-negative bacteria could use carnitine in either aerobic or anaerobic environments for a variety of cellular functions, including as an electron acceptor, as a compatible solute to survive environmental insults or as a sole carbon, nitrogen and energy source. Bacterial carnitine metabolism was most recently reviewed in 1998 (Bieber, 1988;Bremer, 1983;Kleber, 1997;Rebouche & Seim, 1998) and the field has seen important advances. This review summarizes what we knew at the time of the previous reviews and emphasizes what we have learned since, including: (i) how anaerobic bacteria synthesize and utilize crotonobetaine and carnitine as final electron acceptors, (ii) the impact of carnitine degradation by the intestinal microbiota and the genes responsible for this anaerobic conversion, (iii) the genes involved in aerobic degradation of carnitine, and (iv) how carnitine as a compatible solute impacts survival within and outside of the host. Carnitine in the environmentRecent work makes it clear that while animals represent the most readily accessible source of carnitine, carnitine is often present and sometimes abundant in soil and natural waters. Quaternary ammonium compounds are abundant in a number of soil ecosystems, including comprising a quarter of the most abundant organic nitrogen compounds in the soil water of a subalpine gras...

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“…Table 1. Differential characteristics of strains A3F-7 T and ssthio04PA2-7c (Paramoritella gen. nov.) and related genera Data for reference genera were taken from the following studies: Urakawa et al (1998), Nogi et al (1998b), Nogi & Kato (1999), Benediktsdó ttir et al (2000), Lunder et al (2000), Xu et al (2003) and Kim et al (2008) (Moritella); Bowman et al (1997), Bowman (2005), Nogi et al (1998a), Venkateswaran et al (1999), Leonardo et al (1999), Ivanova et al (2001Ivanova et al ( , 2003Ivanova et al ( , 2004a, Bozal et al (2002), Skerratt et al (2002), Brettar et al (2002, Satomi et al (2003Satomi et al ( , 2006Satomi et al ( , 2007, Yoon et al (2004a, b), Toffin et al (2004), Xu et al (2005), Zhao et al (2005Zhao et al ( , 2006Zhao et al ( , 2007 …”

Section: Description Of Paramoritella Alkaliphila Sp Novmentioning

confidence: 99%

Paramoritella alkaliphila gen. nov., sp. nov., a member of the family Moritellaceae isolated in the Republic of Palau

Hosoya

Suzuki

Adachi

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Two strains of marine, heterotrophic, alkaliphilic bacteria, designated A3F-7 T and ssthio04PA2-7c, were isolated from hard coral and marine sand, respectively, collected in the Republic of Palau. A phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were related to members of the genus Moritella. However, the sequence similarities between the isolates and the type strains of the Moritella species were less than 93 %. The G+C contents of the isolates were around 57 mol%, the major respiratory quinone was Q-8 and the predominant cellular fatty acids were 16 : 1v7c, 16 : 0, 18 : 1v7c and 14 : 0. On the basis of the phylogenetic data, phenotypic characteristics and DNA-DNA hybridization results, strains A3F-7 T and ssthio04PA2-7c represent a novel species of a novel genus, for which the name Paramoritella alkaliphila gen. nov., sp. nov. is proposed. The type strain of Paramoritella alkaliphila is A3F-7 T (5MBIC06429 T 5DSM 19956 T ).The genus Moritella, currently the only known member of the family Moritellaceae (Ivanova et al., 2004b), belongs to the order Alteromonadales (Bowman & McMeekin, 2005) within the class Gammaproteobacteria. Members of this genus are characterized as psychrophilic, halophilic, facultatively anaerobic bacteria (Nogi & Kato, 1999; Lunder et al., 2000; Xu et al., 2003). In this study, we used a polyphasic taxonomic approach to investigate two mesophilic strains that were found to represent a novel genus and species belonging to the family Moritellaceae.Strain A3F-7 T was isolated from hard coral (Favites complanata) collected in August 2003 from Iwayama Bay (GPS location 7 u 19.429 N 134 u 29.689 E) in the Republic of Palau. Strain A3F-7 T was isolated by using marine agar 2216 (Difco) adjusted to pH 11 with Na 2 CO 3 and NaHCO 3 . Strain ssthio04PA2-7c was isolated from marine sand collected in the Republic of Palau (GPS location 7 u 20.089 N 134 u 29.799 E) in September 2004. Strain ssthio04PA2-7c was isolated by using a thio medium consisting of a basal medium (l 21 : 23.5 g NaCl, 4.9 g MgCl 2 , 0.2 g CaCl 2 . 2H 2 O, 0.66 g KCl, 0.096 g KBr, 0.026 g H 3 BO 3 , 0.024 g SrCl 2 , 0.003 g NaF, 1.5 % agar, w/v) to which the following components were added (l 21 ): 2 mg sodium pyruvate, 2 mg sodium succinate, 2 mg sodium ascorbate, 2 mg cysteine hydrochloride, 0.002 % (w/v) yeast extract, 25 % (w/v) sodium thiosulfate, 15 ml NaHCO 3 solution (84 g NaHCO 3 l 21 ); 1 ml vitamin solution (20 nM each of biotin, 4-aminobenzoic acid, pantothenate, pyridoxine, nicotinamide, thiamine, lipoic acid, folic acid, vitamin B 12 and riboflavin), 1 ml trace element solution of DSMZ medium 69 (DSMZ, 1993), 1 ml selenite-tungstate solution (0.01 mM Na 2 SeO 3 and 0.01 mM Na 2 WO 4 . 2H 2 O) and 1 ml vitamin B 12 solution (150 mg cyanocobalamin l 21 ). Each isolate was then cultured and maintained on 1/5-diluted marine agar (800 ml filtered seawater, 200 ml distilled water, 7.48 g marine broth and 15 g agar).Nucleotide sequences of the 16S rRNA gene amplified by using primers 27F an...

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Shewanella pacifica sp. nov., a polyunsaturated fatty acid-producing bacterium isolated from sea water

Abstract: ErrataShewanella pacifica sp. nov., a polyunsaturated fatty acid-producing bacterium isolated from sea water

Cited by 11 publications

References 0 publications

Jania rubens-associated bacteria: molecular identification and antimicrobial activity

Jania rubens-associated bacteria: molecular identification and antimicrobial activity

Carnitine in bacterial physiology and metabolism

Paramoritella alkaliphila gen. nov., sp. nov., a member of the family Moritellaceae isolated in the Republic of Palau

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