Shift from morphological to recent advanced molecular approaches for the identification of nematodes

Nisa, Rawhat Un; Tantray, Aadil Yousuf; Shah, Ali Asghar

doi:10.1016/j.ygeno.2022.110295

Cited by 18 publications

(7 citation statements)

References 177 publications

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“…As mentioned above, parasitology requires constant updating since taxonomic classification of organisms may change with new molecular, ecological and morphological findings [ 25 ]. In addition, diagnostics such as DNA-based techniques may be developed with improved sensitivity and specificity as well as simplified accessibility [ 26 , 27 ]. However, interestingly, the popularity of pets may change, like for reptiles and amphibians; thus, updates on their associated parasites and potential role as reservoirs should be studied.…”

Section: Lectures and Practical Activities: From Theory To Practicementioning

confidence: 99%

ParSCo: celebrating 10 years of a unique parasitology summer course

Dantas-Torres,

Bezerra-Santos,

Mendoza-Roldan

et al. 2024

Parasites Vectors

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ParSCo (Parasitology Summer Course) is an intense, 1-week-long summer course organized by the Parasitology Unit of the Department of Veterinary Medicine, University of Bari, Italy, with the support of the World Association for the Advancement of Veterinary Parasitology (WAAVP), the European Veterinary Parasitology College (EVPC) and Parasites and Vectors. The course, which is conducted in southern Italy, is planned for parasitologists and post-graduate students working in the field of parasitology. The course consists of theoretical and practical lessons, which include the collection, identification and diagnosis of parasites of pets, livestock and wildlife. The participants in ParSCo are afforded the opportunity to be involved in clinical examination and sample collection for the diagnosis of parasitic diseases (e.g. leishmaniosis, thelaziosis and many tick-borne diseases) present in the Mediterranean Basin. The course is conducted at Casa di Caccia, a hunting lodge situated in the Gallipoli Cognato Forest near the Basento River in the Basilicata region in southern Italy. In addition to the training purpose, ParSCo is a great opportunity for sharing knowledge and expertise while becoming part of the parasitology community in a pleasant environment. In this editorial, we share some information and celebrate 10 years of ParSCo, looking forward to forthcoming sessions of this unique parasitology summer course. Graphical Abstract

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Section: Lectures and Practical Activities: From Theory To Practicementioning

confidence: 99%

ParSCo: celebrating 10 years of a unique parasitology summer course

Dantas-Torres,

Bezerra-Santos,

Mendoza-Roldan

et al. 2024

Parasites Vectors

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“…Additionally, this is extremely sensitive and carried out using only one adult female’s isolated protein ( Birithia et al., 2012 ). Even though isozyme investigation was commonly used for identification of Meloidogyne ( Nisa et al., 2022 ), more than single polymorphic enzyme was required to authenticate the identification of specific isolates because the presence or absence of an enzyme signal could varywithin and between samples ( Cunha et al., 2018 ).…”

Section: Identification Of Meloidogyne Enterolobiimentioning

confidence: 99%

Meloidogyne enterolobii risk to agriculture, its present status and future prospective for management

Sikandar

Jia

et al. 2023

Front. Plant Sci.

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Meloidogyne enterolobii, commonly known as guava root-knot nematode, poses risk due to its widespread distribution and extensive host range. This species is recognized as the most virulent root-knot nematode (RKN) species because it can emerge and breed in plants that have resistance to other tropical RKNs. They cause chlorosis, stunting, and yield reductions in host plants by producing many root galls. It is extremely challenging for farmers to diagnose due to the symptoms’ resemblance to nutritional inadequacies. This pathogen has recently been considered a significant worldwide threat to agricultural production. It is particularly challenging to diagnose a M. enterolobii due to the similarities between this species and other RKN species. Identified using traditional morphological and molecular techniques, which is a crucial first in integrated management. Chemical control, biological control, the adoption of resistant cultivars, and cultural control have all been developed and effectively utilized to combat root-knot nematodes in the past. The object of this study was to get about the geographical distribution, host plants, symptoms, identification, and control techniques of M. enterolobii and recommend future initiatives to progress its management.

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“…Mitochondrial markers have proven to be a useful tool for the investigation of the phylogeny of different helminths, including toxocarids (Li et al, 2008;Mikaeili et al, 2015;Öge et al, 2019;Fava et al, 2020). They have been used in recent studies to solve nematode systemic problems (Deng et al, 2022;Nisa et al, 2022). In addition to the mitochondrial genes used here, the use of markers for mitochondrially encoded ATP synthase membrane subunit 6 (ATP 6) (Wickramasinghe et al, 2009), NADH dehydrogenase subunit 4 (nad4) (Li et al, 2008) and ITS (Jacobs et al, 1997) genes have also been reportedly successful in the discrimination of species and phylogenetic analysis of the genus Toxocara.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Diversity of helminths with zoonotic potential and molecular characterization of Toxocara canis infecting domestic dogs from locations of Amazon and Atlantic Forest Brazilian biomes

Dias-Correia,

Neves,

Bittencourt-Oliveira

et al. 2023

Rev. Bras. Parasitol. Vet.

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The coproparasitological examination of dogs (n=278) from two Brazilian biomes (Amazon [AZ] and Atlantic Forest [AF]) by centrifugal flotation demonstrated positivity values of 54.2% (AF) and 48.5% (AZ). The most prevalent parasites in AF were hookworms (81.0% - 47/58), Toxocara sp. (17.3% - 10/58) and Trichuris vulpis (12.1% - 7/58); while in AZ they were hookworms (86.7% - 72/83), Toxocara sp. (18.1% - 15/83), Dipylidium caninum (13.3% - 11/83) and T. vulpis (10.8% - 9/83). PCR was performed using the partial mitochondrial genes cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase 1 (pnad1) in 25 fecal samples positive for Toxocara sp. eggs and found one sample positive for pcox1 and six positives for pnad1. The sequencing of these samples was unsuccessful due to the difficulties inherent in copro-PCR+sequencing. The sequencing of 14 samples of T. canis adult helminths retrieved 11 sequences of 414 bp for pcox1 and nine sequences of 358 bp for pnad1. The phylogenetic trees of these sequences confirmed the species T. canis. Intraspecific genetic variation was only observed for pnad1. This is the second study involving molecular analysis of T. canis in dogs from Brazil and adds new information through the use of pnad1.

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Shift from morphological to recent advanced molecular approaches for the identification of nematodes

Cited by 18 publications

References 177 publications

ParSCo: celebrating 10 years of a unique parasitology summer course

ParSCo: celebrating 10 years of a unique parasitology summer course

Meloidogyne enterolobii risk to agriculture, its present status and future prospective for management

Diversity of helminths with zoonotic potential and molecular characterization of Toxocara canis infecting domestic dogs from locations of Amazon and Atlantic Forest Brazilian biomes

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