Shifting the optimal stiffness for cell migration

Bangasser, Benjamin L.; Shamsan, Ghaidan A.; Chan, Clarence E.; Opoku, Kwaku; Tüzel, Erkan; Schlichtmann, Benjamin W.; Kasim, Jesse; Fuller, Benjamin; McCullough, Brannon R.; Rosenfeld, Steven S.; Odde, David J.

doi:10.1038/ncomms15313

Cited by 270 publications

(400 citation statements)

References 31 publications

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“…First, we observed a biphasic trend in cell speed with respect to stiffness. This phenomenon has previously been described in many cell types, including smooth muscle cells [7] and glioma cells in both 2D [48] and 3D [49]. In this migration study, only collagen 1 was present, suggesting that collagen-binding integrins contributed to this mechanosensitive motility after 24 hours [50].…”

Section: Substratessupporting

confidence: 76%

Integrin α₆and EGFR Signaling Converge at Mechanosensitive Calpain 2

Schwartz

Hall

Barney

et al. 2017

Preprint

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Section: Substratessupporting

confidence: 76%

Integrin α₆and EGFR Signaling Converge at Mechanosensitive Calpain 2

Schwartz

Hall

Barney

et al. 2017

Preprint

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“…This was also observed by Pelham et al for epithelial cells and fibroblasts on polyacrylamide gels of varying stiffness . However, the exact mechanisms of how cell behavior depends on the mechanochemical environment remain unknown …”

Section: Resultssupporting

confidence: 69%

Development of Gelatin‐Alginate Hydrogels for Burn Wound Treatment

Stubbe

Mignon

Declercq

et al. 2019

Macromolecular Bioscience

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Hydrogels are interesting as wound dressing for burn wounds to maintain a moist environment. Especially gelatin and alginate based wound dressings show strong potential. Both polymers are modified by introducing photocrosslinkable functionalities and combined to hydrogel films (gel‐MA/alg‐MA). In one protocol gel‐MA films are incubated in alg‐MA solutions and crosslinked afterward into double networks. Another protocol involves blending both and subsequent photocrosslinking. The introduction of alginate into the gelatin matrix results in phase separation with polysaccharide microdomains in a protein matrix. Addition of alg(‐MA) to gel‐MA leads to an increased swelling compared to 100% gelatin and similar to the commercial Aquacel Ag dressing. In vitro tests show better cell adhesion for films which have a lower alginate content and also have superior mechanical properties. The hydrogel dressings exhibit good biocompatibility with adaptable cell attachment properties. An adequate gelatin‐alginate ratio should allow application of the materials as wound dressings for several days without tissue ingrowth.

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“…These issues leave open the possibility that mechanosensing might play a role in the growth and dissemination of gliomas, and identifying optiaml stiffness ranges that alter the phenotype of specific types of glioma cells has potential for new diagnostic and therapeutic efforts. 48 …”

Section: Discussionmentioning

confidence: 99%

Soft Substrates Containing Hyaluronan Mimic the Effects of Increased Stiffness on Morphology, Motility, and Proliferation of Glioma Cells

et al. 2017

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Unlike many other cancer cells that grow in tumors characterized by an abnormally stiff collagen-enriched stroma, glioma cells proliferate and migrate in the much softer environment of the brain, which generally lacks the filamentous protein matrix characteristic of breast, liver, colorectal, and other types of cancer. Glial cell-derived tumors and the cells derived from them are highly heterogeneous and variable in their mechanical properties, their response to treatments, and their properties in vitro. Some glioma samples are stiffer than normal brain when measured ex vivo, but even those that are soft in vitro stiffen after deformation by pressure gradients that arise in the tumor environment in vivo. Such mechanical differences can strongly alter the phenotype of cultured glioma cells. Alternatively, chemical signaling might elicit the same phenotype as increased stiffness by activating intracellular messengers common to both initial stimuli. In this study the responses of three different human glioma cell lines to changes in substrate stiffness are compared with their responses on very soft substrates composed of a combination of hyaluronic acid and a specific integrin ligand, either laminin or collagen I. By quantifying cell morphology, stiffness, motility, proliferation, and secretion of the cytokine IL-8, glioma cell responses to increased stiffness are shown to be nearly identically elicited by substrates containing hyaluronic acid, even in the absence of increased stiffness. PI3-kinase activity was required for the response to hyaluronan but not to stiffness. This outcome suggests that hyaluronic acid can trigger the same cellular response, as can be obtained by mechanical force transduced from a stiff environment, and demonstrates that chemical and mechanical features of the tumor microenvironment can achieve equivalent reactions in cancer cells.

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Shifting the optimal stiffness for cell migration

Cited by 270 publications

References 31 publications

Integrin α₆and EGFR Signaling Converge at Mechanosensitive Calpain 2

Integrin α₆and EGFR Signaling Converge at Mechanosensitive Calpain 2

Development of Gelatin‐Alginate Hydrogels for Burn Wound Treatment

Soft Substrates Containing Hyaluronan Mimic the Effects of Increased Stiffness on Morphology, Motility, and Proliferation of Glioma Cells

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Shifting the optimal stiffness for cell migration

Cited by 270 publications

References 31 publications

Integrin α6and EGFR Signaling Converge at Mechanosensitive Calpain 2

Integrin α6and EGFR Signaling Converge at Mechanosensitive Calpain 2

Development of Gelatin‐Alginate Hydrogels for Burn Wound Treatment

Soft Substrates Containing Hyaluronan Mimic the Effects of Increased Stiffness on Morphology, Motility, and Proliferation of Glioma Cells

Contact Info

Product

Resources

About

Integrin α₆and EGFR Signaling Converge at Mechanosensitive Calpain 2

Integrin α₆and EGFR Signaling Converge at Mechanosensitive Calpain 2