Shikimate pathway activity in shake and fermenter cultures of Cinchona succirubra

Schmauder, H.‐P.; Gröger, D.; Koblitz, H.; Koblitz, D.

doi:10.1007/bf00269365

Cited by 19 publications

(7 citation statements)

References 22 publications

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“…Further work has been undertaken to evaluate the effect of the time of feeding L-tryptophan on alkaloid yield. Other workers [6,[8][9][10][11] have reported L-tryptophan to have a much more pronounced effect on the growth of cell suspension cultures of Cinchona species, which in some instances has even resulted in cell death [11]. When compared with the control flasks a slight decrease in the final fresh weight was noted in the supplemented flasks, comparable to earlier findings [2].…”

Section: Feeding Experiments With L-tryptophansupporting

confidence: 84%

In vitro cultures of Cinchona species. Part II

Hay¹,

Anderson²,

Phillipson³

et al. 1987

Plant Cell Tiss Organ Cult

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The results of preliminary time-course studies on the uptake of radio-labelled alkaloid precursors, L-Imethylene J4C]-tryptophan, [C-5 3H]-secologanin, [C-3 3H]-strictosidine, [Ar 3H]-strictosidine/vincoside and alkaloids, [C-8 3H]-quinine and -quinidine, byCinchona ledgeriana root organs at various stages of the growth cycle are presented. Incorporation of the radio-label from [C-5 3H]-secologanin and [side chain 2 14C]-tryptamine into quinine and quinidine is also reported. Improved alkaloid yields obtained on feeding L-tryptophan have been shown to be dependent on the stage of the growth cycle at which feeding takes place.

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Section: Feeding Experiments With L-tryptophansupporting

confidence: 84%

In vitro cultures of Cinchona species. Part II

Hay¹,

Anderson²,

Phillipson³

et al. 1987

Plant Cell Tiss Organ Cult

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“…Tryptophan feeding, nevertheless, slightly enhances TDC activity; in three separate experiments, the mean TDC activity (day 0-5) in tryptophan-fed seedlings was found to be increased by 39 12% (± SD) compared with untreated control seedlings. A stimulatory influence of tryptophan feeding on TDC activity has been reported for Cinchona cell cultures too (12). However, despite the enhancement in TDC activity in tryptophan-fed seedlings, the level of tryptamine is not very much influenced (Fig.…”

Section: Alkaloid Biosynthesis In Etiolated Seedlingsmentioning

confidence: 93%

“…Interestingly, in one study, tryptophan feeding was reported to have a stimulatory influence on TDC activity (12). On the other hand, the hypothesis has been put forward that increased cellular tryptamine levels have an inhibitory effect on the expression of TDC activity in Catharanthus cell cultures (7).…”

Section: Introductionmentioning

confidence: 98%

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The Influence of Tryptophan and Tryptamine Feeding and Light on Alkaloid Biosynthesis inCinchonaSeedlings

Aerts

Verpoorte²

1992

Planta Med

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It has previously been shown that, at the onset of germination of CINCHONA seeds, transient increases in both the tryptophan level and tryptophan decarboxylase (TDC) activity occur. Subsequently, in the seedlings the levels of the TDC product tryptamine and its derived alkaloids increase. We investigated whether this process can be influenced by external tryptophan or tryptamine feeding. Besides, the possible role of light in alkaloid biosynthesis was studied by monitoring this process in etiolated seedlings. Tryptophan feeding slightly raised the TDC activity in the seedlings, but alkaloid production remained unaltered. During tryptamine feeding, the transient increases in tryptophan level and TDC activity were still observed, and also here, alkaloid production remained unaltered. Finally, in etiolated seedlings the alkaloid biosynthetic pathway proceeded normally. Thus, in germinating CINCHONA seedlings alkaloid production is not susceptible to tryptophan or tryptamine feeding and independent of light.

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“…B. bakterielle Systeme, Zellkulturen von Cinchona succirubra, Ruta graveolens oder Nigella damascena rnit KiWerten um 1-5 pmol/l; SCHMAUDER et al 1985SCHMAUDER et al , 1984HERTEL et al 1988 Maximalgeschwindigkeiten der Enzyme der Alkaloidbiosynthese wesentlich hoher sirid als die Alkaloidsyntheserate in vivo (z. B. LUCKNER und Roos 1982).…”

Section: Regulatorische Eigenschaftenunclassified

Regulation von Schlüsselenzymen der Biosynthese aromatischer Aminosäuren in Alkaloid‐bildenden Kulturen von Penicillium cyclopium w.

Schmauder

Roos

1987

J. Basic Microbiol.

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In Penicdlium cyclopium w., phenylalanin and anthranilate are precursors of the benzodiazepine alkaloids cyclopenin and cyclopenol. The onset and increase of alkaloid biosynthesis in emerged cultures was paralleled by an increase of the in vitro activities of the precursor synthesizing enzymes DAHP synthase'), chorismate mutase, and anthranilate synthase as well as tryptophan synthase. This increase was most pronounced with the DAHP synthase and, therefore, enabled the cells to maintain a constant level of the "central" precursor, chorismate, in the course of alkaloid production. In contrast, the contents of phe, anthranilate as well as tyr and trp decreased with increasing rate of alkaloid synthesis, indicating that the supply of the immediate precursors did not fully compensate the drain towards alkaloid formation. The strongest decrease was seen with the anthranilate content, which was due to several control mechanisms: the increase of anthranilate consuming reactions prior to the anthranilate synthase, the higher capacity of the alternate, phe producing branch of the aromatic pathway, and the feedback inhibition by trp of anthranilate synthase. The formation of anthranilate most probably was the rate limiting step of alkaloid production.

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Shikimate pathway activity in shake and fermenter cultures of Cinchona succirubra

Cited by 19 publications

References 22 publications

In vitro cultures of Cinchona species. Part II

In vitro cultures of Cinchona species. Part II

The Influence of Tryptophan and Tryptamine Feeding and Light on Alkaloid Biosynthesis inCinchonaSeedlings

Regulation von Schlüsselenzymen der Biosynthese aromatischer Aminosäuren in Alkaloid‐bildenden Kulturen von Penicillium cyclopium w.

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