Shoot Induction from Axillary Shoot Tip Explants of Fig (Ficus carica) cv. Japanese BTM 6

Abstract

Fig, or Ficus carica, is a fruit tree from the Moraceae family and is widely grown in tropical and subtropical regions of the world. Fig plants are mainly propagated through grafting, air layering, and hardwood cutting whereby these methods were found to be less efficient. Plant tissue culture is efficient method to propagate plants, particularly to produce true-to-type platelets for mass multiplication. The aim of this study is to induce multiple shoot formation on Ficus carica cv. Japanese BTM 6 through identifying and optimising the concentrations of 6-Benzylaminopurine (BAP) and Zeatin suited for shoot formation. The axillary shoot tip explants were cultured in MS media supplemented with different concentrations of BAP and Zeatin (0, 0.5, 1.0, 1.5 and 2.0 mg/L) to determine the optimal concentration for the formation of multiple shoots. Number of shoots per explants and the differences in shoot height of explants were calculated after 8 and 12 weeks of culture respectively. Of all the treatments of BAP, MS media containing with 2 mg/L BAP marked the highest number of shoots per explant with the average value of 1.67 ± 0.33 while 1.5 and 2 mg/L of BAP produced the highest differences in shoot height with 0.51 ± 0.08 cm and 0.51 ± 0.07 cm after 12 weeks respectively. Murashige and Skoog (MS) media supplemented with 2 mg/L Zeatin showed the highest production of multiple shoots and differences in shoot height with the average of 0.83 ± 0.219 and 0.32 ± 0.04 cm respectively among all the different treatments of Zeatin. In this study, BAP performed better in shoot induction and elongation as compared to Zeatin for the cultivar Japanese BTM 6.

High-frequency regeneration of plants in vitro from seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim

“…In Chlorophytum borivilianum, BAP (2.0~6.0 mg/L) leads to a significant increase in shoot multiplication, and kinetin (2.0~6.0 mg/L) significantly increases shoot elongation (Ashraf et al 2014). In Ficus carica, BAP is more effective for multiple shoot induction from shoot tip explants as compared to zeatin and its optimum concentration was 2 mg/L (Ling et al 2018). In Scutellaria alpine, a comparison of BAP, kinetin, zeatin, and TDZ, found that BAP (0.5~1.0 mg/L) was the most effective for multiple shoot induction from shoot tip explants (Grzegorczyk-Karolak et al 2015).…”

MORFOGENESIS DAN INDUKSI KALUS TIN (Ficus carica L.) PADA MEDIA MURASHIGE DAN SKOOG (MS) DENGAN PENAMBAHAN BENZYLAMINOPURINE

“…Penelitian terdahulu tentang induksi kalus tanaman Tin, diantaranya dilakukan dengan menggunakan kombinasi IBA dan Kinetin (Fadilah et al, 2014), BAP dan Zeatin (Ling et al, 2018). Penelitian ini menggunakan kombinasi BAP dengan air kelapa untuk morfogenesis tunas, dan BAP dengan 2,4-D untuk induksi kalus.…”

“…Hasil pengamatan jumlah daun dan panjang tunas menunjukkan nilai yang baik dan selaras pada perlakuan BAP 2 mg/L + air kelapa 100 mL/L (Tabel 2). Perlakuan tunggal BAP dengan konsentrasi 2 mg/L pada penelitian Ling et al (2018), juga menunjukkan jumlah tunas dan panjang tunas tanaman Tin yang paling baik.…”

Regeneration of Red Palestine FIG (Ficus carica L.) from Formation of Adventitious Shoots in Murashige & Skoog Media with Additional of BAP/TDZ and NAA

“…It is also contain high energy and nutrients, especially P, K, Ca, Mg, and Fe [1]. In addition, figs contain much of fiber which is higher than bananas, grapes, strawberries, apples, and oranges [2]. Figs can be consumed freshly or dried, but fresh figs are perishable and must be eaten or processed immediately.…”

“…The problem on conventional propagation such as grafting is require a lot of planting materials, which can cause problems if it is necessary to produce high quality seeds in large quantities. In addition, conventional vegetative propagation does not produce consistent plant growth, which will result in inconsistent fruit production, especially for largescale markets [2]. Micropropagation/micro propagation by tissue culture techniques, also known as in vitro regeneration, is an alternative method that can be used to multiply plants efficiently.…”