Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Trejgell, Alina; Tretyn, Andrzej

doi:10.2478/v10182-011-0026-z

Cited by 8 publications

(15 citation statements)

References 23 publications

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“…The root explants were not able to produce shoots by organogenesis. Similar results were recorded for Carlina acaulis and C. onopordifolia (Trejgell et al, 2009;Trejgell and Tretyn, 2011). The difference in shoot formation between different types of explants in response to BAP could be related to the levels of endogenous cytokinins (Yucesan et al, 2007) or to differences in tissue sensitivity to plant growth regulators (Lisowska and Wysokinska, 2000).…”

Section: Shoot Multiplicationsupporting

confidence: 65%

“…Moreover, efficient and rapid clonal regeneration method has been shown to be a great tool in the protection of rare and threatened species of the Asteraceae family e.g. Centaurea ultreiae (Mallón et al, 2011), Carlina onopordipholia (Trejgell et al, 2011), Arnica montana (Surmacz-Magdziak and Sugier, 2012), or Taraxacum pieninicum (Trejgell et al, 2013) and others (Amin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Inula germanica L. from the Seedlings Explants

Trejgell

Kamińska

Lisowska

et al. 2018

Not Bot Horti Agrobo

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This is the first communication of micropropagation system for Inula germanica using seedling explants germinated in vitro. The development of this system gives the possibility of future reintroduction of I. germanica providing a way to stabilize or re-establish its population. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from ten-day-old seedlings. Explants were put on MS medium containing 1.0 mg l ) at 26 ± 1 °C. The highest percentage of shoot organogenesis (83.3%) was recorded for hypocotyl, while the highest average number of shoots per explant (12.0) was recorded for shoot tips. In subsequent subcultures, multiplication rate decreased to 3.0-4.9 shoots per explant. Less than 19% shoots were able to root on the solid medium without auxins. The highest rooting efficiency (69.3%) was recorded for solid medium supplemented with indolebutyric acid, but growth of roots was inhibited. The percentage of rooted shoots (62.2%) and number of roots per shoot (2.4 per shoot) into the liquid medium were comparable to medium with 0.1 mg·l -1 indolebutyric acid. showing a positive impact on the process of acclimatization. The regenerated plants were able to flowering in the first year after acclimatization. Developed micropropagation system for I. germanica is efficient and can be a useful tool for the active protection of this species.

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Section: Shoot Multiplicationsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Micropropagation of Inula germanica L. from the Seedlings Explants

Trejgell

Kamińska

Lisowska

et al. 2018

Not Bot Horti Agrobo

Self Cite

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“…In vitro vegetative propagation of declining (critically endangered, E) and vulnerable (V) species of the Polish flora has been attempted in some dicotyledonous species: for example, Arnica montana (SurmaczMagdziak and Sugier, 2012), Carlina onopordifolia (Trejgell and Tretyn, 2011), Drosera anglica (Kawiak and Łojkowska, 2004), D. intermedia (Kromer et al, 2000), D. rotundifolia (Kawiak et al, 2003), Gentiana pneumonanathe (Bach and Pawłowska, 2003), Leontopodium alpinum (Trejgell et al, 2010a), and Senecio macrophyllus (Trejgell et al, 2010b). The monocotyledons selected for such studies include Fritillaria meleagris (Liliaceae), Gladiolus palustris (Iridaceae) and a few Orchidaceae species (Rybczyński and Mikuła, 2006).…”

Section: Introductionmentioning

confidence: 99%

An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

Trejgell¹,

Chernetskyy²,

Podlasiak³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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An efficient micropropagation system for Taraxacum pieninicum using seedling explants germinated in vitro is described. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from several-day-old seedlings. The highest response, 100% frequency with 12.3 axillary shoots/explant, was from shoot tips on medium supplemented with 0.5 mg L -1 BA and 0.05 mg L -1 NAA. In subsequent subcultures the number of shoots was significantly higher on all explants cultured on medium containing 0.25 and 0.5 mg L -1 BA, and the multiplication rate was highest (20 shoots/explant) in the 4th passage. Shoots rooted on MS and 1/2 MS medium; the highest rooting frequency was 90% and the highest number of roots 2.7/shoot. Rooted plants showed 96.2% survival in sterile soil:sand, and 100% survival in hydroponic culture. Regenerated plants flowered in the second year after acclimatization and yielded viable seeds. This protocol for obtaining complete plants through micropropagation may prove useful for conservation of the genetic resources of this and other endangered species.K Ke ey y w wo or rd ds s: : Taraxacum pieninicum, endangered species, in vitro culture, multiple shoots, benzylaminopurine, hydroponic culture, fertile plants, conservation.

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“…This trend was also observed during shoot proliferation of C. acanthifolia subsp. utzka [17]. Moreover, inhibition of shoot elongation by BA has been also reported in C. acaulis [15,16] and other Asteraceae species [46,47].…”

Section: Discussionmentioning

confidence: 83%

“…The results are similar to those regarding C. acaulis where the tested achenes with viable seeds germinate very well (94%) on MS medium supplemented with 2.9 µM GA 3 after 10 days of culture in continuous light (26 ± 1 • C) [15] as well as to those concerning C. acanthifolia subsp. utzka [11,17]. Some phenotypic effects produced by light in plants are induced by PGRs and especially gibberellins (GAs).…”

Section: Discussionmentioning

confidence: 99%

GIS-Facilitated Effective Propagation Protocols of the Endangered Local Endemic of Crete Carlina diae (Rech. f.) Meusel and A. Kástner (Asteraceae): Serving Ex Situ Conservation Needs and Its Future Sustainable Utilization as an Ornamental

Grigoriadou

Sarropoulou

Krigas

et al. 2020

Plants

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Conservation and sustainable exploitation of threatened endemic plants with medicinal and/or horticultural/ornamental value can be achieved through the development of effective propagation protocols. After unveiling the bioclimatic preferences of Carlina diae (Asteraceae) with geographic information systems (GIS), four propagation trials were conducted using seeds of this endangered local Cretan endemic for in vivo and in vitro germination, as well as seasonal vegetative propagation trials (softwood cuttings) and micropropagation (nodal explants). Seed germination was accomplished at a level of 77–90% in vivo (30 days) and 96% in vitro (10 days) using an MS medium with 2.9 μM gibberellic acid (GA3). The optimum treatments for cuttings’ rooting were 1000 and 2000 ppm indole-3-butyric acid (IBA) (11–16 roots, 2–3 cm long, 100% rooting) within 40 days in mist. In vitro shoot propagation exhibited a 2.8 proliferation rate after six successive subcultures on an MS medium with 2.9 μM GA3. Both ex vitro rooting and acclimatization were successful in 40 days, with 96% microshoot rooting and an equal survival rate. The GIS-facilitated effective species-specific propagation protocols developed in this study can consolidate the perspective of successful re-introduction of ex situ-raised material of C. diae into wild habitats and may serve its sustainable exploitation for high-added value ornamental products.

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Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Cited by 8 publications

References 23 publications

Micropropagation of Inula germanica L. from the Seedlings Explants

Micropropagation of Inula germanica L. from the Seedlings Explants

An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

GIS-Facilitated Effective Propagation Protocols of the Endangered Local Endemic of Crete Carlina diae (Rech. f.) Meusel and A. Kástner (Asteraceae): Serving Ex Situ Conservation Needs and Its Future Sustainable Utilization as an Ornamental

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