Shoot Regeneration in Vitro from Diverse Genotypes of Sweetpotato and Multiple Shoot Production per Explant

Abstract

<jats:p>Studies were conducted to improve adventitious shoot regeneration in sweetpotato [<jats:italic>Ipomoea batatas</jats:italic> (L.) Lam.], specifically to extend the protocol to many genotypes and to elicit production of multiple shoots per explant. The use of a two-stage procedure where excised petioles were incubated on Murashige and Skoog (MS) (1962) medium with 2,4-D (0.2 mg·liter<jats:sup>–1</jats:sup>) for 3 days and transferred to a second medium containing MS salts with thidiazuron and 2iP (0.05 mg·liter<jats:sup>–1</jats:sup> each) resulted in shoot regeneration from eight of 13 genotypes tested, including elite sweetpotato cultivars such as `Jewel' and `Rojoblanco'. PI 318846-3 was the most regenerable genotype, with up to 77% of explants producing one to three shoots per explant. The orientation of the petiole on the nutrient medium was critical; those placed vertically inverted developed multiple shoots. Wounding explants through epidermal peeling with normal horizontal orientation of the explants during incubation also resulted in multiple shoot production (about three shoots per explant). Interference with auxin transport due to explant inversion or wounding may have stimulated increased shoot induction. Chemical names used: 2,4 dichlorophenoxyacetic acid (2,4-D); <jats:italic>N</jats:italic>-phenyl-<jats:italic>N</jats:italic>′-1,2,3-thiadiazol-5-ylurea (thidiazuron); <jats:italic>N</jats:italic><jats:sup>6</jats:sup>-(2-isopentenyl) adenine (2iP).</jats:p>

Exogenous and endogenous plant growth regulators’ effect on regeneration of selected African sweetpotato (Ipomoea batatas L. Lam) cultivars

“…resulted in the highest number of cultivars producing multiple shoots. Contrary to our results, other studies reported multiple shoots on media supplemented with TDZ (Gosukonda et al, 1995b;Sefasi et al, 2013). Differences in results could be attributed to the type of cytokinin used and genotypic variations.…”

“…Differences in results could be attributed to the type of cytokinin used and genotypic variations. This notwithstanding, a number of multiple shoots were produced on media containing TDZ in our study, a results reported by Gosukonda et al (1995b) and Sefasi et al (2013).…”

Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

“…Shoot tips are generally preferred for embryogenic procedures (Cavalcante et al 1994;Padmanabhan et al 1998) but leaves, petioles, roots and stems have also been used (Cheng and Yeh 2003;Gong et al 2005). Alternatively, rapid shoot-organogenesis has been obtained from leaf explants Gosukonda et al 1995). Besides tissue regeneration procedures, methods to produce transgenic sweetpotato plants have also been reported.…”

Farklı Besi Ortamlarının Tatlı Patateste in Vitro Çoğaltım Üzerine Etkil

“…Bir çözüm yöntemi olarak in vitro şartlarda çoğaltım, çok sayıda sağlıklı ve uniform bitkiler elde etmeye olanak sağlamaktadır. Tatlı patates somatik embriyogenesis ve harici tomurcuklar yardımıyla apikal ve aksiller tomurcukların gelişimi uyarılarak çoğaltılabilmektedir (Gosukonda et al, 1995, González et al, 1999, Mukherjee, 2002. Ticari alanlar için tohumluk materyali üretmede tomurcuklu eksplantların kullanıldığı teknikler en etkili yöntemlerdendir.…”