Short- and medium-term biological variation estimates of red blood cell and reticulocyte parameters in healthy subjects

Buoro, Sabrina; Carobene, Anna; Seghezzi, Michela; Manenti, Barbara; Dominoni, Paola; Pacioni, Aurelio; Ceriotti, Ferruccio; Ottomano, Cosimo; Lippi, Giuseppe

doi:10.1515/cclm-2017-0902

Cited by 16 publications

(12 citation statements)

References 38 publications

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“…A systematic review identified more than 30 papers delivering BV data for CBC components, but only four of these studies were assigned a BIVAC grade A [19][20][21][22] and the majority a C or D grade. In our study, meta-analysis of BIVAC compliant studies (grade A, B and C) has enabled publication of updated, global estimates of CV I and CV G for CBC parameters.…”

Section: Discussionmentioning

confidence: 99%

“…Due to stability issues, blood samples cannot be stored and, consequently, the measurement of all samples from the same person cannot be performed in a single centre in a single analytical run on the same day, which is the usual recommended approach. Generally, to obtain reliable BV data of CBC parameters, the protocol and the checklist developed by EFLM BV-WG [17,18] should be followed, as in two recently published national studies [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Systematic review and meta-analysis of within-subject and between-subject biological variation estimates of 20 haematological parameters

Coşkun

Braga

Carobene

et al. 2019

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background Interpretation of the complete blood count (CBC) parameters requires reliable biological variation (BV) data. The aims of this study were to appraise the quality of publications reporting BV data for CBC parameters by applying the BV Data Critical Appraisal Checklist (BIVAC) and to deliver global BV estimates based on BIVAC compliant studies. Methods Relevant publications were identified by a systematic literature search and evaluated for their compliance with the 14 BIVAC criteria, scored as A, B, C or D, indicating decreasing compliance. Global CVI and CVG estimates with 95% CI were delivered by a meta-analysis approach using data from BIVAC compliant papers (grades A–C). Results In total, 32 studies were identified; four received a BIVAC grade A, 2 B, 20 C and 6 D. Meta-analysis derived CVI and CVG estimates were generally lower or in line with those published in a historical BV database available online. Except for reticulocytes, CVI estimates of erythrocyte related parameters were below 3%, whereas platelet (except MPV and PDW) and leukocyte related parameters ranged from 5% to 15%. Conclusions A systematic review of CBC parameters has provided updated, global estimates of CVI and CVG that will be included in the newly published European Federation of Clinical Chemistry and Laboratory Medicine BV Database.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systematic review and meta-analysis of within-subject and between-subject biological variation estimates of 20 haematological parameters

Coşkun

Braga

Carobene

et al. 2019

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…5,31,32 Also, intraindividual biological variation for reticulocytes is higher in soccer players compared to inactive subjects. 33,34 Immature reticulocytes are cells with high RNA content which results from the increased rate of bone marrow red cell production. 14 In athletes, high IRF values can be seen in hemolysis, altitude training, and blood manipulation with erythropoietic stimulants.…”

Section: Discussionmentioning

confidence: 99%

“…35 The biological variation for IRF in our soccer players was slightly higher than observed in the non-athletes study population, repeating the same pattern for reticulocytes and RBC parameters. 34…”

Section: Discussionmentioning

confidence: 99%

Reference Change Values for Immature Red Blood Cell Parameters in Under-20 Soccer Players

Nunes¹,

Molina²,

Silva³

2020

Preprint

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Introduction: Immature red blood cell parameters compose of the anti-doping programs of several sports, including soccer. The biological variability of reticulocytes is not well defined in young athletes. Our aim was to calculate biological variation and reference change values (RCV) for immature red blood cell parameters in young soccer players.Methods: Samples from 19 male soccer players (mean age, 18 ± 1 years) were collected before the start of training (C0) and after two (C1) and four weeks of training. Blood samples were collected in tubes with K3-EDTA Vaccuete® (Greiner Bio-One). Red blood cell parameters were analyzed on Sysmex XE-5000®. The e-Check Sysmex® 2 levels were used to obtain the coefficient of analytical variation (CVA). Homogeneity of variance was verified using the Cochran test. The within-subject (CVI) and between-subject biological variation (CVG) was calculated according to the mean and standard deviation from the athletes’ results. ANOVA with repeated measures was used to compare the means at the significance level of p<0.05. RCV95% was calculated using the Fraser’s formula: RCV=21/2 x1.96x(CVA2 + CVI2)1/2. Graph Pad Prism 6.0 and Matlab 7.0 were used to perform statistical analyzes.Results: Mean corpuscular volume, RDW, and reticulocyte hemoglobin content (Ret-He) had significant increases during the training period. The Ret-He were higher in C1 (32.4±1.1 pg) and C2 (32.3±1.3 pg) compared to C0 (31.9±1.2 pg) (p<0.05). It was not possible to calculate RCV for Ret-He because of heterogeneous variances. The RCV of reticulocytes (29.1%) and IRF (48.8%) were higher than Hgb (5.5%) and Hct (6.7%).Conclusion: Reticulocytes are highly variable in different athletes’ and the RCV obtained from young soccer players would contribute to monitor training adaptation and to diagnose sports anemia. The Ret-He behavior in our study suggests that this parameter can detect an early increase of iron for hemoglobin synthesis in response to training, this makes it an interesting biomarker to assess anemia and suspicion of doping by rhEPO.

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“…The evaluation of imprecision was performed by comparison with the analytical performance specifications for imprecision (CV APS ) of CBC parameters, as described by Buoro et al 28‐30 …”

Section: Methodsmentioning

confidence: 99%

Standardization and harmonization in hematology: Instrument alignment, quality control materials, and commutability issue

Vidali¹,

Carobene

Esposito

et al. 2020

Int J Lab Hematology

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Introduction In the hub and spoke laboratory network, the number of hematology analyzers (HAs) within each core center has increased, and the control of HAs alignment is becoming necessary requirement to ensure analytical quality. In this scenario, HA alignment can be assessed by analyzing the same control material used for internal quality control on multiple HAs, assuming its commutability. The aim of the study was to verify the applicability of a protocol for the alignment of HAs based on control material rather than on fresh whole‐blood samples. Methods The alignment of five HAs was evaluated for red (RBC, Hb, MCV, RET), white (WBC, NE, LY, MO, EO, BA, IG), and platelet (PLT) series parameters, following a protocol by SIBioC, using human sample (HS) and quality control material (QC), after the verification of commutability, according to the IFCC protocol. Maximum bias was derived from biological variation data. Results A complete alignment between instruments was confirmed for the majority of the parameters investigated both for HS and QC material. Partial misalignments or inconcludent results were instead evident for MCV, MO, EO, BA, and IG. Interestingly, QC material was found to be not commutable for LY, MO, and BA. Conclusion The alignment of hematologic analyzers for main cell population parameters may be verified with both QC and HS, displaying consistent results and interpretation. The evaluation for some white series parameters (EO, BA, and IG) is critical, and particular attention must be paid to the values of the material used for the alignment.

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Short- and medium-term biological variation estimates of red blood cell and reticulocyte parameters in healthy subjects

Cited by 16 publications

References 38 publications

Systematic review and meta-analysis of within-subject and between-subject biological variation estimates of 20 haematological parameters

Systematic review and meta-analysis of within-subject and between-subject biological variation estimates of 20 haematological parameters

Reference Change Values for Immature Red Blood Cell Parameters in Under-20 Soccer Players

Standardization and harmonization in hematology: Instrument alignment, quality control materials, and commutability issue

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