Short-chain fatty acids alter tight junction permeability in intestinal monolayer cells via lipoxygenase activation

Ohata, Atsushi; Usami, Makoto; Miyoshi, Makoto

doi:10.1016/j.nut.2004.12.004

Cited by 131 publications

(102 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was recently shown that exposure of differentiated Caco-2 cells to prostaglandin E2 resulted in a disruption of epithelial barrier function (42). In addition, cyclooxygenase inhibitors modulated the effects of certain short chain fatty acids on tight junction permeability (43) and restored E-cadherin expression in rat uroepithelium (44). Furthermore, it has been reported that arachidonic acid reduced the transendothelial resistance of endothelial cells (45).…”

Section: Discussionmentioning

confidence: 99%

Melittin as an Epithelial Permeability Enhancer I: Investigation of Its Mechanism of Action in Caco-2 Monolayers

et al. 2007

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Melittin as an Epithelial Permeability Enhancer I: Investigation of Its Mechanism of Action in Caco-2 Monolayers

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Butyrate, in a Caco-2 cell culture model, was also shown to activate AMP-activated protein kinase, resulting in tight junction protein assembly and improved barrier function indicated by increased transepithelial electrical resistance (TEER) (74). Another study that used Caco-2 cells found that butyrate increased lipoxygenase activity by inhibiting histone deacetylation, resulting in increased TEER (75). The importance of fiber has also been recognized in critical care settings, because the use of total enteral or parenteral diets that lack fiber were found to induce gut atrophy and to increase gut permeability; this could be recovered with the addition of fiber or SCFAs (76,77).…”

Section: Impact Of Df On the Gut The Gatekeeper Of The Bodymentioning

confidence: 99%

Impact of Dietary Fibers on Nutrient Management and Detoxification Organs: Gut, Liver, and Kidneys

Kieffer

Martin

Adams

2016

Advances in Nutrition

View full text Add to dashboard Cite

Increased dietary fiber (DF) intake elicits a wide range of physiologic effects, not just locally in the gut, but systemically. DFs can greatly alter the gut milieu by affecting the gut microbiome, which in turn influences the gut barrier, gastrointestinal immune and endocrine responses, and nitrogen cycling and microbial metabolism. These gut-associated changes can then alter the physiology and biochemistry of the body's other main nutrient management and detoxification organs, the liver and kidneys. The molecular mechanisms by which DF alters the physiology of the gut, liver, and kidneys is likely through gut-localized events (i.e., bacterial nitrogen metabolism, microbe-microbe, and microbe-host cell interactions) coupled with specific factors that emanate from the gut in response to DF, which signal to or affect the physiology of the liver and kidneys. The latter may include microbe-derived xenometabolites, peptides, or bioactive food components made available by gut microbes, inflammation signals, and gut hormones. The intent of this review is to summarize how DF alters the gut milieu to specifically affect intestinal, liver, and kidney functions and to discuss the potential local and systemic signaling networks that are involved. Adv Nutr 2016;7:1111-21.

show abstract

“…However, FAs and MGs derived from long-chain triglycerides (LCTs, >C12) are re-esterified, incorporated into chylomicrons, and enter the lymphatic system, bypassing the hepatic first-pass metabolism [32]. SCTs are known to induce tight junction permeability changes [33], while the micellar solubilisation capacity of MCTs and LCTs has been proven to be higher than that of SCTs [26,34]. Although the assessment of the performance of TGs with different chain lengths has been carried out before, these studies have only focused on the end result, i.e.…”

Section: Introductionmentioning

confidence: 99%

Chain length affects pancreatic lipase activity and the extent and pH–time profile of triglyceride lipolysis

Benito-Gallo

Franceschetto

Wong

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

View full text Add to dashboard Cite

Triglycerides (TG) are one of the most common excipients used in oral lipid-based formulations. The chain length of the TG plays an important role in the oral bioavailability of the co-administered drug. Fatty acid (FA) chain-length specificity of porcine pancreatic lipase was studied by means of an in vitro lipolysis model under bio-relevant conditions at pH 6.80. In order to determine the total extent of lipolysis, back-titration experiments at pH 11.50 were performed. Results suggest that there is a specific chain length range (C2-C8) for which pancreatic lipase shows higher activity. This specificity could result from a combination of physicochemical properties of TGs, 2-monoglycerides (2-MGs) and FAs, namely the droplet size of the TGs, the solubility of 2-MGs within mixed micelles, and the relative stability of the FAs as leaving groups in the hydrolysis reaction. During experimentation, it was evident that an optimisation of lipolysis conditions was needed for tighter control over pH levels so as to better mimic in vivo conditions. 1M NaOH, 3.5 mL/min maximum dosing rate, and 3 μL/min minimum dosing rate were the optimised set of conditions that allowed better pH control, as well as the differentiation of the lipolysis of different lipid loads.

show abstract

Short-chain fatty acids alter tight junction permeability in intestinal monolayer cells via lipoxygenase activation

Abstract: These results suggest that short-chain fatty acids, especially butyrate, induce TJ permeability changes through LOX activation through histone acetylation.

Cited by 131 publications

References 26 publications

Melittin as an Epithelial Permeability Enhancer I: Investigation of Its Mechanism of Action in Caco-2 Monolayers

Melittin as an Epithelial Permeability Enhancer I: Investigation of Its Mechanism of Action in Caco-2 Monolayers

Impact of Dietary Fibers on Nutrient Management and Detoxification Organs: Gut, Liver, and Kidneys

Chain length affects pancreatic lipase activity and the extent and pH–time profile of triglyceride lipolysis

Contact Info

Product

Resources

About