Short Communication Development and characterization of microsatellite loci for genetic studies of the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

Pavinato, Vitor Antonio Corrêa; Silva-Brandão, Karina Lucas; Monteiro, Marisa C; Zucchi, Maria Imaculada; Pinheiro, José Baldin; Dias, Fábio Luís Ferreira; Omoto, Celso

doi:10.4238/2013.may.14.3

Cited by 5 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Before extraction, all larvae were dissected to remove parasitoid pupae. Microsatellite genotyping was performed following (Pavinato et al 2013) using the loci Dsc1, Dsc2, Dsc3, Dsc5, Dsc7, Dsc9, Dsc10, Dsc11, Dsc19 and Dsc20 (the annealing temperature of Dsc5 was changed to 56°C). Each microsatellite loci was genotyped separately and the alleles were scored on a 6% polyacrylamide gel with an Analyzer 4300 s Li-Cor (Biosciences, Lincoln, NE, USA).…”

Section: Sampling Dna Extraction and Microsatellite Genotypingmentioning

confidence: 99%

Influence of historical land use and modern agricultural expansion on the spatial and ecological divergence of sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae) in Brazil

et al. 2017

View full text Add to dashboard Cite

Human-mediated changes in landscapes can facilitate niche expansion and accelerate the adaptation of insect species. The interaction between the evolutionary history of the sugarcane borer, Diatraea saccharalis Fabricius, and historical and modern agricultural activity in Brazil shaped its spatial genetic structure, facilitating ecological divergence and incipient host shifting. Based on microsatellite data, STRUCTURE analyses identified two (K = 2) and three (K = 3) significant genetic clusters that corresponded to: (a) a strong signal of spatial genetic structure and, (b) a cryptic signal of host differentiation. We inferred that K = 2 reflects the footprint of agricultural activity, such as expansion of crop production (sugarcane and maize), unintentional dispersion of pests, and management practices. In contrast, K = 3 indicated incipient host differentiation between larvae collected from sugarcane or maize. Our estimates of population size changes indicated that a historical bottleneck was associated with a reduction of sugarcane production ≈200 years ago. However, a more recent population expansion was detected (>1950s), associated with agricultural expansion of large crop production into previously unfarmed land. Partial Mantel tests supported our hypothesis of incipient host adaptation, and identified isolation-by-environment (e.g., host plant) in São Paulo and Minas Gerais states, where sugarcane has been traditionally produced in Brazil. The impact of agricultural production on D. saccharalis may continue, as the current population structure may hinder the efficacy of refuge plants in delaying insect resistance evolution to Bt toxin.

show abstract

Section: Sampling Dna Extraction and Microsatellite Genotypingmentioning

confidence: 99%

Influence of historical land use and modern agricultural expansion on the spatial and ecological divergence of sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae) in Brazil

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The microsatellite loci used in the study were provided by the Laboratory of Conservation Genetics and Genomics, Agribusiness Technological Development of São Paulo, Brazil and were developed by Pavinato [ 57 ]. Details and characteristics of the microsatellites are shown in S1 Table .…”

Section: Methodsmentioning

confidence: 99%

“…Details and characteristics of the microsatellites are shown in S1 Table . Microsatellite amplification conditions and gel separation were performed according to Pavinato [ 57 ].…”

Section: Methodsmentioning

confidence: 99%

Morphological and molecular characterization of Brazilian populations of Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) and the evolutionary relationship among species of Diatraea Guilding

et al. 2017

View full text Add to dashboard Cite

The sugarcane borer or corn stalk borer, Diatraea Guilding is polyphagous insect pest of many important crops such as corn, sorghum and sugarcane. Losses arising from the attack of Diatraea species have been a serious problem, which may cause loss in sugarcane production around 0.25% in sugar, 0.20% in alcohol and 0.77% of body weight for every 1% infestation and up to 21% in corn production fields. In Brazil, the most commonly reported species are Diatraea saccharalis (Fabricius, 1794) and Diatraea impersonatella (Walker, 1863) (= D. flavipennella). However, multiple other species of Diatraea have been identified in Brazil according to the literature. Currently, little information exists on the presence of the other species causing injury to sugarcane and corn. The objectives of this study were to improve the accuracy of species assignment, evaluate the population genetic structure, and address many of the outstanding questions of systematics and evolution of Brazilian populations of D. saccharalis. To address these main questions, classical taxonomic methods were used, focused on morphological characterization of the reproductive organs, especially the male genitalia. In addition, genetic studies were performed using simple sequence repeats (SSR) and a fragment of cytochrome C oxidase subunit I (COI) gene. The data and findings from this research will contribute to the understanding of evolutionary aspects of insect pests in order to develop more effective and sustainable population management practices.

show abstract

“…Molecular markers available for this species are scarce, and they are limited to a set of 12 microsatellite loci (Pavinato et al . ), 96 AFLP markers (Joyce et al . ), 216 RAPD and 39 sex‐linked RAPD markers (Heideman et al .…”

Section: Introductionmentioning

confidence: 99%

“…Molecular markers available for this species are scarce, and they are limited to a set of 12 microsatellite loci (Pavinato et al 2013), 96 AFLP markers (Joyce et al 2014), 216 RAPD and 39 sex-linked RAPD markers (Heideman et al 2010;Lopes et al 2014) and, more recently, one barcode primer set (V. A. C. Pavinato, J. B. Campos & M. I. Zucchi, unpublished, Joyce et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Restriction site associated DNA (RAD) for de novo sequencing and marker discovery in sugarcane borer, Diatraea saccharalis Fab. (Lepidoptera: Crambidae)

Pavinato

Margarido

Wijeratne

et al. 2016

Molecular Ecology Resources

View full text Add to dashboard Cite

We present the development of a genomic library using RADseq (restriction site associated DNA sequencing) protocol for marker discovery that can be applied on evolutionary studies of the sugarcane borer Diatraea saccharalis, an important South American insect pest. A RADtag protocol combined with Illumina paired-end sequencing allowed de novo discovery of 12 811 SNPs and a high-quality assembly of 122.8M paired-end reads from six individuals, representing 40 Gb of sequencing data. Approximately 1.7 Mb of the sugarcane borer genome distributed over 5289 minicontigs were obtained upon assembly of second reads from first reads RADtag loci where at least one SNP was discovered and genotyped. Minicontig lengths ranged from 200 to 611 bp and were used for functional annotation and microsatellite discovery. These markers will be used in future studies to understand gene flow and adaptation to host plants and control tactics.

show abstract

Short Communication Development and characterization of microsatellite loci for genetic studies of the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

Cited by 5 publications

References 16 publications

Influence of historical land use and modern agricultural expansion on the spatial and ecological divergence of sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae) in Brazil

Influence of historical land use and modern agricultural expansion on the spatial and ecological divergence of sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae) in Brazil

Morphological and molecular characterization of Brazilian populations of Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) and the evolutionary relationship among species of Diatraea Guilding

Restriction site associated DNA (RAD) for de novo sequencing and marker discovery in sugarcane borer, Diatraea saccharalis Fab. (Lepidoptera: Crambidae)

Contact Info

Product

Resources

About