Short Communication:  Molecular barcode and morphology analysis of Malva pseudolavatera Webb &amp; Berthel and Malva sylvestris L. from Ecuador

Sarmiento-Tomalá, Glenda; Santos-Ordóñez, Efrén; Miranda-Martínez, Migdalia; Pacheco-Coello, Ricardo; Scull-Lizama, Ramón; Gutiérrez-Gaitén, Yamilet; Hernández, René Delgado

doi:10.13057/biodiv/d210818

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…The clade for D. adscendens CS had a 99% support while the D. molliculum CS clade was supported by only 61%, one of the lowest percentages obtained in this research. Despite the fact that the ITS2 marker has been reported to be successful in identifying specimens to the species level by up to 92.7% (24) and other studies on medicinal plants highly recommend the use of the ITS markers for species identi cation and differentiation (23,(25)(26)(27)(28), we found that is not the best marker for D. molliculum identi cation.…”

Section: Discussioncontrasting

confidence: 59%

DNA barcode analysis and antioxidant activity of Desmodium molliculum and Desmodium adscendens

Sarmiento-Pacurucu,

Santos-Ordóñez,

León-Tamariz

et al. 2024

Preprint

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In Ecuador, two species of Desmodium are used as traditional medicine. These are popularly known as “hierba del infante” and refer to D. adscendens and D. molliculum. The first species has a large base of information on its genetics and biological activity on which its traditional use is supported, while the second, D. molliculum, lacks this scientific information. This research aims to establish a base knowledge for the species D. molliculum: characterize the species genetically by obtaining the molecular DNA barcodes trnh-psbA, rbcL, matK, ITS1 and ITS2, evaluate its antioxidant effect and compare it with that of D. adscendens using the in vitro techniques of Folin-Ciocalteu, ABTS, DPPH and FRAP to assess its potential medicinal effect. De novo genetic DNA barcodes were obtained for the species D. molliculum and the phylogenetic analysis separated them from those obtained from D. adscendens, indicating that the analyzed species can be discriminated by DNA barcodes. In addition, the methanolic extracts of D. molliculum contain more than double the content of total polyphenols (30.1 ± 1.1 mg GAE/g dry plant) than those of D. adscendens (13.82 ± 0.74 mg AG/g dry plant) as well as better performance in all antioxidation assays. Additionally, this research established that the defatted extract of D. molliculum has the highest antioxidant activity in the ABTS (1.16 ± 0.001 mg TE/mg) and FRAP (0.39 ± 0.01 mg TE/mg) assays.

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Section: Discussioncontrasting

confidence: 59%

DNA barcode analysis and antioxidant activity of Desmodium molliculum and Desmodium adscendens

Sarmiento-Pacurucu,

Santos-Ordóñez,

León-Tamariz

et al. 2024

Preprint

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“…Qualitative and quantitative differences were observed between both samples. These differences were associated to intrinsic factors, mainly to the genetical variability inter-species, previously reported (Sarmiento et al, 2020a).…”

Section: Phytochemical Compositionmentioning

confidence: 66%

Phytochemical composition and antioxidant capacity of the aqueous extracts of Malva sylvestris L. and Malva pseudolavatera Webb & Berthel.

Tomalá¹,

Gaitén²,

Hernández³

et al. 2022

J Pharm Pharmacogn Res

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Context: Malva spp. have been widely used in the world as traditional remedies. In Ecuadorian markers Malva sylvestris and Malva pseudolavatera are the species most commercialized. However, the scientific information about M. pseudolavatera is little. Aims: To determine the chemical composition and antioxidant capacity of the aqueous extracts of the leaves of both species. Methods: Aqueous extracts were partitioned with butanol and the fractions obtained were analyzed by GC/MS. Total extracts were evaluated as antioxidants using FRAP, DPPH and ABTS assays. Results: Differences in the chemical composition were found between the extracts. Both extracts showed the presence of polysaccharides, phenolic acids and fatty acids, but in M. sylvestris were identified polyols, which there was not observed in M. pseudolavatera. The aqueous extracts showed free radical scavenging and ferric reducing power capacities in vitro assays, being M. sylvestris the most promissory antioxidant, possibly due to the presence of polyols in the extract. In the present research were reported 39 phytochemical compounds by first time for the specie M. pseudolavatera. Conclusions: Aqueous extracts of the leaves of M. sylvestris and M. pseudolavatera showed antioxidant capacity associated with the presence of phenolic acids, polysaccharides and flavonoids in the extracts.

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“…The ITS2 revealed a low percentage of identification (80.49%) with BLASTn, suggesting that species differentiation could be detected using the ITS2. The results from other studies have indicated a better resolution for species identification using the ITS2 in medicinal plants (Techen et al, 2014;Zhang et al, 2016;Bustamante et al, 2019;Sarmiento-Tomalá et al, 2020).…”

Section: Molecular Barcode Sequencesmentioning

confidence: 96%

Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

Soledispa

Santos-Ordóñez

Miranda

et al. 2021

PeerJ

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Smilax plants are distributed in tropical, subtropical, and temperate regions in both hemispheres of the world. They are used extensively in traditional medicines in a number of countries. However, morphological and molecular barcodes analysis, which may assist in the taxonomic identification of species, are lacking in Ecuador. In order to evaluate the micromorphological characteristics of these plants, cross sections of Smilax purhampuy leaves were obtained manually. The rhizome powder, which is typically used in traditional medicines, was analyzed for micromorphological characteristics. All samples were clarified with 1% sodium hypochlorite. Tissues were colored with 1% safranin in water and were fixed with glycerinated gelatin. DNA was extracted from the leaves using a modified CTAB method for molecular barcode characterization and PCR was performed using primers to amplify the different loci including the plastid genome regions atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer; and the nuclear DNA sequence ITS2. A DNA sequence similarity search was performed using BLAST in the GenBank nr database and phylogenetic analysis was performed using the maximum likelihood method according to the best model identified by MEGAX using a bootstrap test with 1,000 replicates. Results showed that the micromorphological evaluation of a leaf cross section depicted a concave arrangement of the central vein, which was more pronounced in the lower section and had a slight protuberance. The micromorphological analysis of the rhizome powder allowed the visualization of a group of cells with variable sizes in the parenchyma and revealed thickened xylematic vessels associated with other elements of the vascular system. Specific amplicons were detected in DNA barcoding for all the barcodes tested except for the trnH–psbA spacer. BLAST analysis revealed that the Smilax species was predominant in all the samples for each barcode; therefore, the genus Smilax was confirmed through DNA barcode analysis. The barcode sequences psbK-psbI, atpF-atpH, and ITS2 had a better resolution at the species level in phylogenetic analysis than the other barcodes we tested.

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Short Communication: Molecular barcode and morphology analysis of Malva pseudolavatera Webb & Berthel and Malva sylvestris L. from Ecuador

Cited by 4 publications

References 18 publications

DNA barcode analysis and antioxidant activity of Desmodium molliculum and Desmodium adscendens

DNA barcode analysis and antioxidant activity of Desmodium molliculum and Desmodium adscendens

Phytochemical composition and antioxidant capacity of the aqueous extracts of Malva sylvestris L. and Malva pseudolavatera Webb & Berthel.

Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

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